Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants
Aluminum (Al) toxicity and phosphorus (P) deficiency are widely recognized as major constraints to agricultural productivity in acidic soils. Under this scenario, the development of ryegrass plants with enhanced P use efficiency and Al resistance is a promising approach by which to maintain pasture...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/929 |
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| author | Leyla Parra-Almuna Sofía Pontigo Antonieta Ruiz Felipe González Nuria Ferrol María de la Luz Mora Paula Cartes |
| author_facet | Leyla Parra-Almuna Sofía Pontigo Antonieta Ruiz Felipe González Nuria Ferrol María de la Luz Mora Paula Cartes |
| author_sort | Leyla Parra-Almuna |
| collection | DOAJ |
| description | Aluminum (Al) toxicity and phosphorus (P) deficiency are widely recognized as major constraints to agricultural productivity in acidic soils. Under this scenario, the development of ryegrass plants with enhanced P use efficiency and Al resistance is a promising approach by which to maintain pasture production. In this study, we assessed the contribution of growth traits, P efficiency, organic acid anion (OA) exudation, and the expression of Al-responsive genes in improving tolerance to concurrent low-P and Al stress in ryegrass (<i>Lolium perenne</i> L.). Ryegrass plants were hydroponically grown under optimal (0.1 mM) or low-P (0.01 mM) conditions for 21 days, and further supplied with Al (0 and 0.2 mM) for 3 h, 24 h and 7 days. Accordingly, higher Al accumulation in the roots and lower Al translocation to the shoots were found in ryegrass exposed to both stresses. Aluminum toxicity and P limitation did not change the OA exudation pattern exhibited by roots. However, an improvement in the root growth traits and P accumulation was found, suggesting an enhancement in Al tolerance and P efficiency under combined Al and low-P stress. Al-responsive genes were highly upregulated by Al stress and P limitation, and also closely related to P utilization efficiency. Overall, our results provide evidence of the specific strategies used by ryegrass to co-adapt to multiple stresses in acid soils. |
| first_indexed | 2024-04-24T10:36:50Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T10:36:50Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-69e398f61597482f9cf511a822e7f3a22024-04-12T13:24:39ZengMDPI AGPlants2223-77472024-03-0113792910.3390/plants13070929Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass PlantsLeyla Parra-Almuna0Sofía Pontigo1Antonieta Ruiz2Felipe González3Nuria Ferrol4María de la Luz Mora5Paula Cartes6Center of Plant Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChileCenter of Plant Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChileDepartamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChilePrograma de Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChileDepartamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, SpainCenter of Plant Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChileCenter of Plant Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, P.O. Box 54-D, Temuco 4811230, ChileAluminum (Al) toxicity and phosphorus (P) deficiency are widely recognized as major constraints to agricultural productivity in acidic soils. Under this scenario, the development of ryegrass plants with enhanced P use efficiency and Al resistance is a promising approach by which to maintain pasture production. In this study, we assessed the contribution of growth traits, P efficiency, organic acid anion (OA) exudation, and the expression of Al-responsive genes in improving tolerance to concurrent low-P and Al stress in ryegrass (<i>Lolium perenne</i> L.). Ryegrass plants were hydroponically grown under optimal (0.1 mM) or low-P (0.01 mM) conditions for 21 days, and further supplied with Al (0 and 0.2 mM) for 3 h, 24 h and 7 days. Accordingly, higher Al accumulation in the roots and lower Al translocation to the shoots were found in ryegrass exposed to both stresses. Aluminum toxicity and P limitation did not change the OA exudation pattern exhibited by roots. However, an improvement in the root growth traits and P accumulation was found, suggesting an enhancement in Al tolerance and P efficiency under combined Al and low-P stress. Al-responsive genes were highly upregulated by Al stress and P limitation, and also closely related to P utilization efficiency. Overall, our results provide evidence of the specific strategies used by ryegrass to co-adapt to multiple stresses in acid soils.https://www.mdpi.com/2223-7747/13/7/929aluminum toxicityphosphorus deficiencyphosphorus use efficiencyaluminum resistancealuminum-responsive genesryegrass |
| spellingShingle | Leyla Parra-Almuna Sofía Pontigo Antonieta Ruiz Felipe González Nuria Ferrol María de la Luz Mora Paula Cartes Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants Plants aluminum toxicity phosphorus deficiency phosphorus use efficiency aluminum resistance aluminum-responsive genes ryegrass |
| title | Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants |
| title_full | Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants |
| title_fullStr | Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants |
| title_full_unstemmed | Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants |
| title_short | Dissecting the Roles of Phosphorus Use Efficiency, Organic Acid Anions, and Aluminum-Responsive Genes under Aluminum Toxicity and Phosphorus Deficiency in Ryegrass Plants |
| title_sort | dissecting the roles of phosphorus use efficiency organic acid anions and aluminum responsive genes under aluminum toxicity and phosphorus deficiency in ryegrass plants |
| topic | aluminum toxicity phosphorus deficiency phosphorus use efficiency aluminum resistance aluminum-responsive genes ryegrass |
| url | https://www.mdpi.com/2223-7747/13/7/929 |
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