Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes
BackgroundBoth lactylation and m6A modification have important implications for the development of clear cell renal cell carcinoma (ccRCC), and we aimed to use crosstalk genes of both to reveal the prognostic and immunological features of ccRCC.MethodsOur first step was to look for lactylation-relat...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1225023/full |
| _version_ | 1797744773115674624 |
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| author | Lin Yang Xiaoyu Wang Jiahao Liu Xiaoqiang Liu Sheng Li Fuchun Zheng Qianxi Dong Songhui Xu Jing Xiong Bin Fu |
| author_facet | Lin Yang Xiaoyu Wang Jiahao Liu Xiaoqiang Liu Sheng Li Fuchun Zheng Qianxi Dong Songhui Xu Jing Xiong Bin Fu |
| author_sort | Lin Yang |
| collection | DOAJ |
| description | BackgroundBoth lactylation and m6A modification have important implications for the development of clear cell renal cell carcinoma (ccRCC), and we aimed to use crosstalk genes of both to reveal the prognostic and immunological features of ccRCC.MethodsOur first step was to look for lactylation-related genes that differed between normal and tumor tissues, and then by correlation analysis, we found the genes associated with M6A. Following that, ccRCC subtypes will be identified and risk models will be constructed to compare the prognosis and tumor microenvironment among different subgroups. A nomogram was constructed to predict the prognosis of ccRCC, and in vitro, experiments were conducted to validate the expression and function of key genes.ResultsWe screened 100 crosstalk genes and identified 2 ccRCC subtypes. A total of 11 prognostic genes were screened for building a risk model. we observed higher immune scores, elevated tumor mutational burden, and microsatellite instability scores in the high-risk group. Therefore, individuals classified as high-risk would derive greater benefits from immunotherapy. The nomogram’s ability to predict overall survival with a 1-year AUC of 0.863 demonstrates its significant practical utility. In addition, HIBCH was identified as a potential therapeutic target and its expression and function were verified by in vitro experiments.ConclusionIn addition to developing a precise prognostic nomogram for patients with ccRCC, our study also discovered the potential of HIBCH as a biomarker for the disease. |
| first_indexed | 2024-03-12T15:14:02Z |
| format | Article |
| id | doaj.art-69e5c300872f4577a87421d8f202b776 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T15:14:02Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-69e5c300872f4577a87421d8f202b7762023-08-11T16:53:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12250231225023Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genesLin YangXiaoyu WangJiahao LiuXiaoqiang LiuSheng LiFuchun ZhengQianxi DongSonghui XuJing XiongBin FuBackgroundBoth lactylation and m6A modification have important implications for the development of clear cell renal cell carcinoma (ccRCC), and we aimed to use crosstalk genes of both to reveal the prognostic and immunological features of ccRCC.MethodsOur first step was to look for lactylation-related genes that differed between normal and tumor tissues, and then by correlation analysis, we found the genes associated with M6A. Following that, ccRCC subtypes will be identified and risk models will be constructed to compare the prognosis and tumor microenvironment among different subgroups. A nomogram was constructed to predict the prognosis of ccRCC, and in vitro, experiments were conducted to validate the expression and function of key genes.ResultsWe screened 100 crosstalk genes and identified 2 ccRCC subtypes. A total of 11 prognostic genes were screened for building a risk model. we observed higher immune scores, elevated tumor mutational burden, and microsatellite instability scores in the high-risk group. Therefore, individuals classified as high-risk would derive greater benefits from immunotherapy. The nomogram’s ability to predict overall survival with a 1-year AUC of 0.863 demonstrates its significant practical utility. In addition, HIBCH was identified as a potential therapeutic target and its expression and function were verified by in vitro experiments.ConclusionIn addition to developing a precise prognostic nomogram for patients with ccRCC, our study also discovered the potential of HIBCH as a biomarker for the disease.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1225023/fulllactylationM6Aclear cell renal cell carcinomaimmunotherapytumor microenvironment |
| spellingShingle | Lin Yang Xiaoyu Wang Jiahao Liu Xiaoqiang Liu Sheng Li Fuchun Zheng Qianxi Dong Songhui Xu Jing Xiong Bin Fu Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes Frontiers in Immunology lactylation M6A clear cell renal cell carcinoma immunotherapy tumor microenvironment |
| title | Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes |
| title_full | Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes |
| title_fullStr | Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes |
| title_full_unstemmed | Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes |
| title_short | Prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6A and lactylation modification-related genes |
| title_sort | prognostic and tumor microenvironmental feature of clear cell renal cell carcinoma revealed by m6a and lactylation modification related genes |
| topic | lactylation M6A clear cell renal cell carcinoma immunotherapy tumor microenvironment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1225023/full |
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