Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama
<i>Klebsiella pneumoniae</i> has been among the main pathogens contributing to the burden of antimicrobial resistance (AMR) in the last decade, and <i>K. pneumoniae</i> AMR strains predominantly cluster in the ST258 clonal complex. However, ST307 is emerging as an important h...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/12/1817 |
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| author | Virginia Núñez-Samudio Gumercindo Pimentel-Peralta Mellissa Herrera Maydelin Pecchio Johana Quintero Iván Landires |
| author_facet | Virginia Núñez-Samudio Gumercindo Pimentel-Peralta Mellissa Herrera Maydelin Pecchio Johana Quintero Iván Landires |
| author_sort | Virginia Núñez-Samudio |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> has been among the main pathogens contributing to the burden of antimicrobial resistance (AMR) in the last decade, and <i>K. pneumoniae</i> AMR strains predominantly cluster in the ST258 clonal complex. However, ST307 is emerging as an important high-risk clone. In Central America, there have been few studies on the molecular epidemiology of the <i>K. pneumoniae</i> strains involved in infections. Materials and Methods: We conducted an epidemiological study in three reference hospitals in the central region of Panama, using isolates of <i>K. pneumoniae</i> involved in infections, and identifying their AMR profile, associated clinical risk factors, and molecular typing using a multilocus sequence typing (ST) scheme. Results: Six STs were detected: 307 (55%), 152, 18, 29, 405, and 207. CTX-M-15- and TEM-type beta-lactamases were identified in 100% of ESBL-producing strains; substitutions in <i>gyrA</i> Ser83Ile and parC Ser80Ile were identified in all ST307s; and in ST152 gyrA Ser83Phe, Asp87Ala, and parC Ser80Ile, the <i>qnrB</i> gene was detected in all strains resistant to ciprofloxacin. Conclusions: We present the first report on ST307 in three reference hospitals in the central region of Panama, which is a high-risk emerging clone and represents a public health alert for potential difficulties in managing <i>K. pneumoniae</i> infections in Panama, and which may extend to other Central American countries. |
| first_indexed | 2024-03-09T17:24:00Z |
| format | Article |
| id | doaj.art-69e699941a324e959894d91d6b1738e0 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T17:24:00Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-69e699941a324e959894d91d6b1738e02023-11-24T12:54:55ZengMDPI AGAntibiotics2079-63822022-12-011112181710.3390/antibiotics11121817Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central PanamaVirginia Núñez-Samudio0Gumercindo Pimentel-Peralta1Mellissa Herrera2Maydelin Pecchio3Johana Quintero4Iván Landires5Instituto de Ciencias Médicas, Las Tablas, Los Santos 0710, PanamaInstituto de Ciencias Médicas, Las Tablas, Los Santos 0710, PanamaLaboratorio Clínico, Hospital Luis Chicho Fábrega, Región de Salud Veraguas, Ministry of Health, Santiago, Veraguas 0923, PanamaInstituto de Ciencias Médicas, Las Tablas, Los Santos 0710, PanamaInstituto de Ciencias Médicas, Las Tablas, Los Santos 0710, PanamaInstituto de Ciencias Médicas, Las Tablas, Los Santos 0710, Panama<i>Klebsiella pneumoniae</i> has been among the main pathogens contributing to the burden of antimicrobial resistance (AMR) in the last decade, and <i>K. pneumoniae</i> AMR strains predominantly cluster in the ST258 clonal complex. However, ST307 is emerging as an important high-risk clone. In Central America, there have been few studies on the molecular epidemiology of the <i>K. pneumoniae</i> strains involved in infections. Materials and Methods: We conducted an epidemiological study in three reference hospitals in the central region of Panama, using isolates of <i>K. pneumoniae</i> involved in infections, and identifying their AMR profile, associated clinical risk factors, and molecular typing using a multilocus sequence typing (ST) scheme. Results: Six STs were detected: 307 (55%), 152, 18, 29, 405, and 207. CTX-M-15- and TEM-type beta-lactamases were identified in 100% of ESBL-producing strains; substitutions in <i>gyrA</i> Ser83Ile and parC Ser80Ile were identified in all ST307s; and in ST152 gyrA Ser83Phe, Asp87Ala, and parC Ser80Ile, the <i>qnrB</i> gene was detected in all strains resistant to ciprofloxacin. Conclusions: We present the first report on ST307 in three reference hospitals in the central region of Panama, which is a high-risk emerging clone and represents a public health alert for potential difficulties in managing <i>K. pneumoniae</i> infections in Panama, and which may extend to other Central American countries.https://www.mdpi.com/2079-6382/11/12/1817epidemiologymolecular geneticsantimicrobial resistance<i>Klebsiella pneumoniae</i>emerging clone ST307Panama |
| spellingShingle | Virginia Núñez-Samudio Gumercindo Pimentel-Peralta Mellissa Herrera Maydelin Pecchio Johana Quintero Iván Landires Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama Antibiotics epidemiology molecular genetics antimicrobial resistance <i>Klebsiella pneumoniae</i> emerging clone ST307 Panama |
| title | Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama |
| title_full | Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama |
| title_fullStr | Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama |
| title_full_unstemmed | Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama |
| title_short | Molecular Genetic Epidemiology of an Emerging Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> Clone (ST307) Obtained from Clinical Isolates in Central Panama |
| title_sort | molecular genetic epidemiology of an emerging antimicrobial resistant i klebsiella pneumoniae i clone st307 obtained from clinical isolates in central panama |
| topic | epidemiology molecular genetics antimicrobial resistance <i>Klebsiella pneumoniae</i> emerging clone ST307 Panama |
| url | https://www.mdpi.com/2079-6382/11/12/1817 |
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