| Summary: | Introduction: Today regarding drug resistance of fungi and bacteria, many researches are focused
on herbal-based medication. As these herbal-based medications can show better adaptivity, the
minimum advantage of them compared to synthetic drugs is that they are harmless. This article
aimed to study the antifungal effect of alcoholic extract and essence of Arnebia euchroma L
(Abukhalsa) roots on saprophytic and dermatophytic fungi.
Methods: In this research, the roots were collected from Zagros heights in spring. Then they
were dried and 300 mL ethanol was added to each 100 g dried powder. The alcoholic extraction
was performed by maceration and the extract was concentrated by distillation in vacuum. The
clevenger apparatus was used to extract the essence; then it was extracted by boiling water at
vacuum for 4–6 hours. Shikonin was provided in commercial form. The antifungal activities of
alcoholic extract, essence and Shikonin were studied and recorded using cylinder test based on
the diameter of inhibition zone in Sabouraud-Dextrose agar. Minimum inhibitory concentration
(MIC) and Minimum fungicidal concentration (MFC) were measured by broths macrodilution
tests.
Results: The results from cylinder, MIC and MFC tests showed that 30% of shikonin was more
effective than root on fungi. Our data demonstrated that alcoholic extract was better than oily
extract.
Conclusion: The alcoholic extract had better characteristics than the essence. To confirm the
final findings, further researches are required.
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