Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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Bibliographic Details
Main Authors: George Chennell, Robin J. W. Willows, Sean C. Warren, David Carling, Paul M. W. French, Chris Dunsby, Alessandro Sardini
Format: Article
Language:English
Published: MDPI AG 2016-08-01
Series:Sensors
Subjects:
FRET
FLIM
AMPK
spheroid
2-photon
biosensor
TCSPC
3D culture
Online Access:http://www.mdpi.com/1424-8220/16/8/1312

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