Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose
Abstract Background Petal blotch is a unique ornamental trait in angiosperm families, and blotch in rose petal is rare and has great esthetic value. However, the cause of the formation of petal blotch in rose is still unclear. The influence of key enzyme genes and regulatory genes in the pigment syn...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-01-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04057-6 |
| _version_ | 1797946019724394496 |
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| author | Naizhe Ji Qianyu Wang Shanshan Li Jiaxin Wen Liangsheng Wang Xiaohao Ding Shiwei Zhao Hui Feng |
| author_facet | Naizhe Ji Qianyu Wang Shanshan Li Jiaxin Wen Liangsheng Wang Xiaohao Ding Shiwei Zhao Hui Feng |
| author_sort | Naizhe Ji |
| collection | DOAJ |
| description | Abstract Background Petal blotch is a unique ornamental trait in angiosperm families, and blotch in rose petal is rare and has great esthetic value. However, the cause of the formation of petal blotch in rose is still unclear. The influence of key enzyme genes and regulatory genes in the pigment synthesis pathways needs to be explored and clarified. Results In this study, the rose cultivar ‘Sunset Babylon Eyes’ with rose-red to dark red blotch at the base of petal was selected as the experimental material. The HPLC-DAD and UPLC-TQ-MS analyses indicated that only cyanidin 3,5-O-diglucoside (Cy3G5G) contributed to the blotch pigmentation of ‘Sunset Babylon Eyes’, and the amounts of Cy3G5G varied at different developmental stages. Only flavonols but no flavone were found in blotch and non-blotch parts. As a consequence, kaempferol and its derivatives as well as quercetin and its derivatives may act as background colors during flower developmental stages. Despite of the differences in composition, the total content of carotenoids in blotch and non-blotch parts were similar, and carotenoids may just make the petals show a brighter color. Transcriptomic data, quantitative real-time PCR and promoter sequence analyses indicated that RC7G0058400 (F3’H), RC6G0470600 (DFR) and RC7G0212200 (ANS) may be the key enzyme genes for the early formation and color deepening of blotch at later stages. As for two transcription factor, RC7G0019000 (MYB) and RC1G0363600 (WRKY) may bind to the promoters of critical enzyme genes, or RC1G0363600 (WRKY) may bind to the promoter of RC7G0019000 (MYB) to activate the anthocyanin accumulation in blotch parts of ‘Sunset Babylon Eyes’. Conclusions Our findings provide a theoretical basis for the understanding of the chemical and molecular mechanism for the formation of petal blotch in rose. |
| first_indexed | 2024-04-10T21:04:16Z |
| format | Article |
| id | doaj.art-69f559d5c65e4cd8894d061ac1b908ae |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-10T21:04:16Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-69f559d5c65e4cd8894d061ac1b908ae2023-01-22T12:08:40ZengBMCBMC Plant Biology1471-22292023-01-0123111610.1186/s12870-023-04057-6Metabolic profile and transcriptome reveal the mystery of petal blotch formation in roseNaizhe Ji0Qianyu Wang1Shanshan Li2Jiaxin Wen3Liangsheng Wang4Xiaohao Ding5Shiwei Zhao6Hui Feng7Beijing Key Lab of Greening Plants Breeding, Beijing Academy of Forestry and Landscape ArchitectureKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesCollege of Forestry, Henan Agricultural UniversityKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesCollege of Food Science, Fuyang Normal UniversityBeijing Key Lab of Greening Plants Breeding, Beijing Academy of Forestry and Landscape ArchitectureBeijing Key Lab of Greening Plants Breeding, Beijing Academy of Forestry and Landscape ArchitectureAbstract Background Petal blotch is a unique ornamental trait in angiosperm families, and blotch in rose petal is rare and has great esthetic value. However, the cause of the formation of petal blotch in rose is still unclear. The influence of key enzyme genes and regulatory genes in the pigment synthesis pathways needs to be explored and clarified. Results In this study, the rose cultivar ‘Sunset Babylon Eyes’ with rose-red to dark red blotch at the base of petal was selected as the experimental material. The HPLC-DAD and UPLC-TQ-MS analyses indicated that only cyanidin 3,5-O-diglucoside (Cy3G5G) contributed to the blotch pigmentation of ‘Sunset Babylon Eyes’, and the amounts of Cy3G5G varied at different developmental stages. Only flavonols but no flavone were found in blotch and non-blotch parts. As a consequence, kaempferol and its derivatives as well as quercetin and its derivatives may act as background colors during flower developmental stages. Despite of the differences in composition, the total content of carotenoids in blotch and non-blotch parts were similar, and carotenoids may just make the petals show a brighter color. Transcriptomic data, quantitative real-time PCR and promoter sequence analyses indicated that RC7G0058400 (F3’H), RC6G0470600 (DFR) and RC7G0212200 (ANS) may be the key enzyme genes for the early formation and color deepening of blotch at later stages. As for two transcription factor, RC7G0019000 (MYB) and RC1G0363600 (WRKY) may bind to the promoters of critical enzyme genes, or RC1G0363600 (WRKY) may bind to the promoter of RC7G0019000 (MYB) to activate the anthocyanin accumulation in blotch parts of ‘Sunset Babylon Eyes’. Conclusions Our findings provide a theoretical basis for the understanding of the chemical and molecular mechanism for the formation of petal blotch in rose.https://doi.org/10.1186/s12870-023-04057-6RoseBlotchAnthocyaninFlavonolTranscriptome |
| spellingShingle | Naizhe Ji Qianyu Wang Shanshan Li Jiaxin Wen Liangsheng Wang Xiaohao Ding Shiwei Zhao Hui Feng Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose BMC Plant Biology Rose Blotch Anthocyanin Flavonol Transcriptome |
| title | Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| title_full | Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| title_fullStr | Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| title_full_unstemmed | Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| title_short | Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| title_sort | metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose |
| topic | Rose Blotch Anthocyanin Flavonol Transcriptome |
| url | https://doi.org/10.1186/s12870-023-04057-6 |
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