GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis
GLUT4 protein expression in white adipose tissue (WAT) and skeletal muscle (SM) was investigated in 2-month-old, 12-month-old spontaneously obese or 12-month-old calorie-restricted lean Wistar rats, by considering different parameters of analysis, such as tissue and body weight, and total protein yi...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2001-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000018 |
| _version_ | 1828901025243725824 |
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| author | P.M. Seraphim M.T. Nunes U.F. Machado |
| author_facet | P.M. Seraphim M.T. Nunes U.F. Machado |
| author_sort | P.M. Seraphim |
| collection | DOAJ |
| description | GLUT4 protein expression in white adipose tissue (WAT) and skeletal muscle (SM) was investigated in 2-month-old, 12-month-old spontaneously obese or 12-month-old calorie-restricted lean Wistar rats, by considering different parameters of analysis, such as tissue and body weight, and total protein yield of the tissue. In WAT, a ~70% decrease was observed in plasma membrane and microsomal GLUT4 protein, expressed as µg protein or g tissue, in both 12-month-old obese and 12-month-old lean rats compared to 2-month-old rats. However, when plasma membrane and microsomal GLUT4 tissue contents were expressed as g body weight, they were the same. In SM, GLUT4 protein content, expressed as µg protein, was similar in 2-month-old and 12-month-old obese rats, whereas it was reduced in 12-month-old obese rats, when expressed as g tissue or g body weight, which may play an important role in insulin resistance. Weight loss did not change the SM GLUT4 content. These results show that altered insulin sensitivity is accompanied by modulation of GLUT4 protein expression. However, the true role of WAT and SM GLUT4 contents in whole-body or tissue insulin sensitivity should be determined considering not only GLUT4 protein expression, but also the strong morphostructural changes in these tissues, which require different types of data analysis. |
| first_indexed | 2024-12-13T15:55:42Z |
| format | Article |
| id | doaj.art-69f7104b77584dadbc5328c0ecc237a6 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-13T15:55:42Z |
| publishDate | 2001-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-69f7104b77584dadbc5328c0ecc237a62022-12-21T23:39:20ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2001-10-0134101353136210.1590/S0100-879X2001001000018GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysisP.M. SeraphimM.T. NunesU.F. MachadoGLUT4 protein expression in white adipose tissue (WAT) and skeletal muscle (SM) was investigated in 2-month-old, 12-month-old spontaneously obese or 12-month-old calorie-restricted lean Wistar rats, by considering different parameters of analysis, such as tissue and body weight, and total protein yield of the tissue. In WAT, a ~70% decrease was observed in plasma membrane and microsomal GLUT4 protein, expressed as µg protein or g tissue, in both 12-month-old obese and 12-month-old lean rats compared to 2-month-old rats. However, when plasma membrane and microsomal GLUT4 tissue contents were expressed as g body weight, they were the same. In SM, GLUT4 protein content, expressed as µg protein, was similar in 2-month-old and 12-month-old obese rats, whereas it was reduced in 12-month-old obese rats, when expressed as g tissue or g body weight, which may play an important role in insulin resistance. Weight loss did not change the SM GLUT4 content. These results show that altered insulin sensitivity is accompanied by modulation of GLUT4 protein expression. However, the true role of WAT and SM GLUT4 contents in whole-body or tissue insulin sensitivity should be determined considering not only GLUT4 protein expression, but also the strong morphostructural changes in these tissues, which require different types of data analysis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000018insulin resistanceobesityweight lossGLUT4 protein |
| spellingShingle | P.M. Seraphim M.T. Nunes U.F. Machado GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis Brazilian Journal of Medical and Biological Research insulin resistance obesity weight loss GLUT4 protein |
| title | GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis |
| title_full | GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis |
| title_fullStr | GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis |
| title_full_unstemmed | GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis |
| title_short | GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis |
| title_sort | glut4 protein expression in obese and lean 12 month old rats insights from different types of data analysis |
| topic | insulin resistance obesity weight loss GLUT4 protein |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000018 |
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