Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling
Objective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais. Methods: Dried mulberry leaves, dried mulberry leaves with 50% raw silkworm pupa and raw silkworm pupa were fermented with Cord...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=12;spage=557;epage=565;aulast=Guo |
| _version_ | 1818665829769674752 |
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| author | Lu Guo Jum Soon Kang Young Hoon Park Beong Il Je Dae Youn Hwang Woo Hong Joo Young Whan Choi |
| author_facet | Lu Guo Jum Soon Kang Young Hoon Park Beong Il Je Dae Youn Hwang Woo Hong Joo Young Whan Choi |
| author_sort | Lu Guo |
| collection | DOAJ |
| description | Objective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais.
Methods: Dried mulberry leaves, dried mulberry leaves with 50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25 °C, after which the dried mulberry leaves and fermented product were extracted with 70% ethanol and subjected to high performance liquid chromatography (HPLC). The contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid were determined. We then used the 3T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro.
Results: HPLC showed that fermentation changed the contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid. Furthermore, fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves, fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption. Additionally, fermented dried mulberry leaves with 50% raw silkworm pupa inhibited PPAR-γ signaling.
Conclusions: Fermentation with Cordyceps militaris enhanced anti-adipogenesis efficacy of mulberry leaves. |
| first_indexed | 2024-12-17T05:54:51Z |
| format | Article |
| id | doaj.art-69fe24ea33934a0e8b82a62c0066a1d6 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-17T05:54:51Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-69fe24ea33934a0e8b82a62c0066a1d62022-12-21T22:01:04ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462020-01-01131255756510.4103/1995-7645.296724Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signalingLu GuoJum Soon KangYoung Hoon ParkBeong Il JeDae Youn HwangWoo Hong JooYoung Whan ChoiObjective: To establish an efficacious and efficient fermentation method of enhancing the anti-adipogenesis effect of mulberry (Morus alba) leaves using Cordyceps militais. Methods: Dried mulberry leaves, dried mulberry leaves with 50% raw silkworm pupa and raw silkworm pupa were fermented with Cordyceps militais for 4 weeks at 25 °C, after which the dried mulberry leaves and fermented product were extracted with 70% ethanol and subjected to high performance liquid chromatography (HPLC). The contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid were determined. We then used the 3T3-L1 cells to investigate whether extracts of fermentation enhanced anti-adipogenesis activity in vitro. Results: HPLC showed that fermentation changed the contents of cordycepin, pelargonidin, chlorogenic acid, iso-quercetin and caffeic acid. Furthermore, fermented dried mulberry leaves with 50% raw silkworm pupa had a better efficacy of anti-adipogenesis than dried mulberry leaves, fermented dried mulberry leaves and fermented silkworm pupa and inhibited triglycerides accumulation and glucose consumption. Additionally, fermented dried mulberry leaves with 50% raw silkworm pupa inhibited PPAR-γ signaling. Conclusions: Fermentation with Cordyceps militaris enhanced anti-adipogenesis efficacy of mulberry leaves.http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=12;spage=557;epage=565;aulast=Guomulberry leaves; cordyceps militaris; fermentation; 3t3-l1 cell; anti-adipogenesis; ppar-γ |
| spellingShingle | Lu Guo Jum Soon Kang Young Hoon Park Beong Il Je Dae Youn Hwang Woo Hong Joo Young Whan Choi Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling Asian Pacific Journal of Tropical Medicine mulberry leaves; cordyceps militaris; fermentation; 3t3-l1 cell; anti-adipogenesis; ppar-γ |
| title | Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling |
| title_full | Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling |
| title_fullStr | Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling |
| title_full_unstemmed | Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling |
| title_short | Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling |
| title_sort | fermentation of mulberry leaves with cordyceps militaris enhanced anti adipogenesis activity in 3t3 l1 cells through down regulation of ppar γ pathway signaling |
| topic | mulberry leaves; cordyceps militaris; fermentation; 3t3-l1 cell; anti-adipogenesis; ppar-γ |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=12;spage=557;epage=565;aulast=Guo |
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