Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization
Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the pres...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2023-01-01
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| Series: | Journal of Tropical Biodiversity and Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/jtbb/article/view/75128 |
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| author | Abdulaziz Dardau Aliyu Muskhazli Mustafa Nor Azwady Abd Aziz Yap Chee Kong Najaatu Shehu Hadi |
| author_facet | Abdulaziz Dardau Aliyu Muskhazli Mustafa Nor Azwady Abd Aziz Yap Chee Kong Najaatu Shehu Hadi |
| author_sort | Abdulaziz Dardau Aliyu |
| collection | DOAJ |
| description | Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the present study, the potential of indigenous soil urease-producing bacteria as potential agents for soil stabilization methods was investigated. Assessment of the eight active urease-producing bacterial species isolated from the farm soil samples has demonstrated that all the isolates were Gram-positive rod-shaped bacteria with promising characteristics such as the formation of endospore which is essential for bacterial survival in harsh conditions within the soil environment. The pH profile and growth profile of the isolates were studied and urease activity was measured by the phenol hypochlorite assay method. Two isolates designated isolate O6w and isolate O3a were selected based on the highest urease activity recorded at 665 U/mL and 620 U/mL, respectively, and they were able to increase and sustain alkaline culture condition (pH 8.71 ± 0.01 and 8.55 ± 0.01) which was suitable for CaCO3 precipitation. The isolates were identified based on 16S ribosomal RNA sequencing to be Bacillus cereus (O6w) and Bacillus paramycoides (O3a). This current study suggested that indigenous soil ureolytic bacteria are potential raw material for the biotreatment of soils stability. |
| first_indexed | 2024-03-12T22:54:00Z |
| format | Article |
| id | doaj.art-69ff98dc45ee42068727b67f222370c7 |
| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-03-12T22:54:00Z |
| publishDate | 2023-01-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-69ff98dc45ee42068727b67f222370c72023-07-20T03:12:55ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812023-01-018110.22146/jtbb.7512833161Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil StabilizationAbdulaziz Dardau Aliyu0Muskhazli Mustafa1Nor Azwady Abd Aziz2Yap Chee Kong3Najaatu Shehu Hadi4Department of Biology, Faculty of Science University Putra Malaysia; Department of Microbiology, Faculty of Science, Federal University of Lafia, NigeriaDepartment of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia.Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia.Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia.Department of Microbiology, Faculty of Science, Federal University of Lafia, Akunza 950101, Nasarawa State, Nigeria.Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the present study, the potential of indigenous soil urease-producing bacteria as potential agents for soil stabilization methods was investigated. Assessment of the eight active urease-producing bacterial species isolated from the farm soil samples has demonstrated that all the isolates were Gram-positive rod-shaped bacteria with promising characteristics such as the formation of endospore which is essential for bacterial survival in harsh conditions within the soil environment. The pH profile and growth profile of the isolates were studied and urease activity was measured by the phenol hypochlorite assay method. Two isolates designated isolate O6w and isolate O3a were selected based on the highest urease activity recorded at 665 U/mL and 620 U/mL, respectively, and they were able to increase and sustain alkaline culture condition (pH 8.71 ± 0.01 and 8.55 ± 0.01) which was suitable for CaCO3 precipitation. The isolates were identified based on 16S ribosomal RNA sequencing to be Bacillus cereus (O6w) and Bacillus paramycoides (O3a). This current study suggested that indigenous soil ureolytic bacteria are potential raw material for the biotreatment of soils stability.https://jurnal.ugm.ac.id/jtbb/article/view/75128micpurease enzymecalcium carbonateureolytic bacteriaproblematic soils |
| spellingShingle | Abdulaziz Dardau Aliyu Muskhazli Mustafa Nor Azwady Abd Aziz Yap Chee Kong Najaatu Shehu Hadi Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization Journal of Tropical Biodiversity and Biotechnology micp urease enzyme calcium carbonate ureolytic bacteria problematic soils |
| title | Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization |
| title_full | Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization |
| title_fullStr | Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization |
| title_full_unstemmed | Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization |
| title_short | Assessing Indigenous Soil Ureolytic Bacteria as Potential Agents for Soil Stabilization |
| title_sort | assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| topic | micp urease enzyme calcium carbonate ureolytic bacteria problematic soils |
| url | https://jurnal.ugm.ac.id/jtbb/article/view/75128 |
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