| Summary: | We measured and studied the growth parameters and the qualitative and quantitative composition of the flavones of hairy roots of the <i>Scutellaria</i> genus: <i>S. lateriflora</i>, <i>S. przewalskii</i> and <i>S. pycnoclada</i>. Hairy roots were obtained using wild-type <i>Agrobacterium rhizogenes</i> A4 by co-cultivation of explants (cotyledons) in a suspension of <i>Agrobacterium</i>. The presence of the <i>rol</i>-genes was confirmed by PCR analysis. The hairy roots of the most studied plant from the <i>Scutellaria</i> genus, <i>S. baicalensis,</i> were obtained earlier and used as a reference sample. HPLC-MS showed the predominance of four main flavones (baicalin, baicalein, wogonin and wogonoside) in the methanol extracts of the studied hairy roots. In addition to the four main flavones, the other substances which are typical to the aerial part of plants were found in all the extracts: apigenin, apigetrin, scutellarin and chrysin-7-<i>O</i>-β-<span style="font-variant: small-caps;">d</span>-glucuronide. According to the total content of flavones, the hairy roots of the studied skullcaps form the following series: <i>S. przewalskii</i> (33 mg/g dry weight) > <i>S. baicalensis</i> (17.04 mg/g dry weight) > <i>S. pycnoclada</i> (12.9 mg/g dry weight) > <i>S. lateriflora</i> (4.57 mg/g dry weight). Therefore, the most promising producer of anti-coronavirus flavones is <i>S. przewalskii.</i>
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