Unraveling the Possibilities: Recent Progress in DNA Biosensing
Due to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitation...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/9/889 |
| _version_ | 1827726932176797696 |
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| author | Meng Yu Tingli He Qianqian Wang Cheng Cui |
| author_facet | Meng Yu Tingli He Qianqian Wang Cheng Cui |
| author_sort | Meng Yu |
| collection | DOAJ |
| description | Due to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitations in sensitivity, selectivity, and limit of detection. Consequently, they have attracted significant attention from researchers worldwide. Here, we exemplify four foundational categories of functional nucleic acids: aptamers, DNAzymes, i-motifs, and G-quadruplexes, from the perspective of the structure-driven functionality in constructing DNA biosensors. Furthermore, we provide a concise overview of the design and detection mechanisms employed in these DNA biosensors. Noteworthy advantages of DNA as a sensor component, including its programmable structure, reaction predictility, exceptional specificity, excellent sensitivity, and thermal stability, are highlighted. These characteristics contribute to the efficacy and reliability of DNA biosensors. Despite their great potential, challenges remain for the successful application of DNA biosensors, spanning storage and detection conditions, as well as associated costs. To overcome these limitations, we propose potential strategies that can be implemented to solve these issues. By offering these insights, we aim to inspire subsequent researchers in related fields. |
| first_indexed | 2024-03-10T22:59:21Z |
| format | Article |
| id | doaj.art-6a005ba2f20a4f528dd8a4892baa0828 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T22:59:21Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6a005ba2f20a4f528dd8a4892baa08282023-11-19T09:47:43ZengMDPI AGBiosensors2079-63742023-09-0113988910.3390/bios13090889Unraveling the Possibilities: Recent Progress in DNA BiosensingMeng Yu0Tingli He1Qianqian Wang2Cheng Cui3Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, ChinaDue to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitations in sensitivity, selectivity, and limit of detection. Consequently, they have attracted significant attention from researchers worldwide. Here, we exemplify four foundational categories of functional nucleic acids: aptamers, DNAzymes, i-motifs, and G-quadruplexes, from the perspective of the structure-driven functionality in constructing DNA biosensors. Furthermore, we provide a concise overview of the design and detection mechanisms employed in these DNA biosensors. Noteworthy advantages of DNA as a sensor component, including its programmable structure, reaction predictility, exceptional specificity, excellent sensitivity, and thermal stability, are highlighted. These characteristics contribute to the efficacy and reliability of DNA biosensors. Despite their great potential, challenges remain for the successful application of DNA biosensors, spanning storage and detection conditions, as well as associated costs. To overcome these limitations, we propose potential strategies that can be implemented to solve these issues. By offering these insights, we aim to inspire subsequent researchers in related fields.https://www.mdpi.com/2079-6374/13/9/889DNA biosensoraptasensorDNAzymei-motifG-quadruplex |
| spellingShingle | Meng Yu Tingli He Qianqian Wang Cheng Cui Unraveling the Possibilities: Recent Progress in DNA Biosensing Biosensors DNA biosensor aptasensor DNAzyme i-motif G-quadruplex |
| title | Unraveling the Possibilities: Recent Progress in DNA Biosensing |
| title_full | Unraveling the Possibilities: Recent Progress in DNA Biosensing |
| title_fullStr | Unraveling the Possibilities: Recent Progress in DNA Biosensing |
| title_full_unstemmed | Unraveling the Possibilities: Recent Progress in DNA Biosensing |
| title_short | Unraveling the Possibilities: Recent Progress in DNA Biosensing |
| title_sort | unraveling the possibilities recent progress in dna biosensing |
| topic | DNA biosensor aptasensor DNAzyme i-motif G-quadruplex |
| url | https://www.mdpi.com/2079-6374/13/9/889 |
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