A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry
Summary: Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ. In addition, the protocol is compatible with m...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200658X |
| _version_ | 1797980427024072704 |
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| author | Zhengyuan Pang Li Ye |
| author_facet | Zhengyuan Pang Li Ye |
| author_sort | Zhengyuan Pang |
| collection | DOAJ |
| description | Summary: Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ. In addition, the protocol is compatible with molecular marker staining for cell type identifications.For complete details on the use and execution of this protocol, please refer to Pang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T05:54:18Z |
| format | Article |
| id | doaj.art-6a02e22ca28a4d52ae24e0f9b2cf2bd8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T05:54:18Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-6a02e22ca28a4d52ae24e0f9b2cf2bd82022-12-22T04:41:57ZengElsevierSTAR Protocols2666-16672022-12-0134101778A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistryZhengyuan Pang0Li Ye1Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA; Corresponding authorDepartment of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA; Corresponding authorSummary: Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ. In addition, the protocol is compatible with molecular marker staining for cell type identifications.For complete details on the use and execution of this protocol, please refer to Pang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200658XNeuroscienceBiotechnology and bioengineering |
| spellingShingle | Zhengyuan Pang Li Ye A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry STAR Protocols Neuroscience Biotechnology and bioengineering |
| title | A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| title_full | A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| title_fullStr | A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| title_full_unstemmed | A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| title_short | A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| title_sort | protocol to visualize on target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry |
| topic | Neuroscience Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200658X |
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