Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat
Breast cancer is the most common cancer in females. The aim of this study was to determine the effect of paclitaxel (PTX) and doxorubicin (DOX) therapy on the βIII-tubulin, carbonic anhydrase IX (CA IX), and survivin expression in chemically-induced rat mammary tumors. Animals with induced mammary c...
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2021-06-01
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author | Alena Pastornická Silvia Rybárová Slávka Drahošová Jozef Mihalik Andrea Kreheľová Andriana Pavliuk-Karachevtseva Ingrid Hodorová |
author_facet | Alena Pastornická Silvia Rybárová Slávka Drahošová Jozef Mihalik Andrea Kreheľová Andriana Pavliuk-Karachevtseva Ingrid Hodorová |
author_sort | Alena Pastornická |
collection | DOAJ |
description | Breast cancer is the most common cancer in females. The aim of this study was to determine the effect of paclitaxel (PTX) and doxorubicin (DOX) therapy on the βIII-tubulin, carbonic anhydrase IX (CA IX), and survivin expression in chemically-induced rat mammary tumors. Animals with induced mammary carcinogenesis were randomly divided into treatment groups and an untreated group. The total proportion of tumors, the proportion of carcinoma in situ (CIS), and invasive carcinoma (IC) were evaluated. Protein expression in tumor tissue was determined using IHC. Statistical analysis of the data, evaluated by Fisher-exact test and unpaired <i>t</i>-test. Significantly increased levels of proteins in the tumor cells were confirmed using the IHC method for all studied proteins. The expression of βIII-tubulin, CA IX, and survivin increased significantly after treatment with both cytostatics (PTX and DOX). Depending on the type of tumor, a significant increase in all proteins was observed in IC samples after PTX treatment, and CA IX expression after DOX treatment. In CIS samples, a significant increase of βIII-tubulin and survivin expression was observed after a DOX treatment. The results suggest that βIII-tubulin, survivin, and CA IX may be significant drug resistance markers and the clinical regulation of their activity may be an effective means of reversing this resistance. |
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issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-10T10:25:25Z |
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spelling | doaj.art-6a0445df47f24a659acc04ce0280b7de2023-11-22T00:03:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212636310.3390/ijms22126363Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female RatAlena Pastornická0Silvia Rybárová1Slávka Drahošová2Jozef Mihalik3Andrea Kreheľová4Andriana Pavliuk-Karachevtseva5Ingrid Hodorová6Department of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaDepartment of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaDepartment of Pathological Anatomy, Jessenius Faculty of Medicine, Comenius University, Kollárova 2, 036 59 Martin, SlovakiaDepartment of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaDepartment of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaDepartment of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaDepartment of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 041 83 Košice, SlovakiaBreast cancer is the most common cancer in females. The aim of this study was to determine the effect of paclitaxel (PTX) and doxorubicin (DOX) therapy on the βIII-tubulin, carbonic anhydrase IX (CA IX), and survivin expression in chemically-induced rat mammary tumors. Animals with induced mammary carcinogenesis were randomly divided into treatment groups and an untreated group. The total proportion of tumors, the proportion of carcinoma in situ (CIS), and invasive carcinoma (IC) were evaluated. Protein expression in tumor tissue was determined using IHC. Statistical analysis of the data, evaluated by Fisher-exact test and unpaired <i>t</i>-test. Significantly increased levels of proteins in the tumor cells were confirmed using the IHC method for all studied proteins. The expression of βIII-tubulin, CA IX, and survivin increased significantly after treatment with both cytostatics (PTX and DOX). Depending on the type of tumor, a significant increase in all proteins was observed in IC samples after PTX treatment, and CA IX expression after DOX treatment. In CIS samples, a significant increase of βIII-tubulin and survivin expression was observed after a DOX treatment. The results suggest that βIII-tubulin, survivin, and CA IX may be significant drug resistance markers and the clinical regulation of their activity may be an effective means of reversing this resistance.https://www.mdpi.com/1422-0067/22/12/6363βIII-tubulinbreast cancercarbonic anhydrase IXdoxorubicinpaclitaxelsurvivin |
spellingShingle | Alena Pastornická Silvia Rybárová Slávka Drahošová Jozef Mihalik Andrea Kreheľová Andriana Pavliuk-Karachevtseva Ingrid Hodorová Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat International Journal of Molecular Sciences βIII-tubulin breast cancer carbonic anhydrase IX doxorubicin paclitaxel survivin |
title | Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat |
title_full | Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat |
title_fullStr | Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat |
title_full_unstemmed | Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat |
title_short | Influence of Paclitaxel and Doxorubicin Therapy of ßIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat |
title_sort | influence of paclitaxel and doxorubicin therapy of ssiii tubulin carbonic anhydrase ix and survivin in chemically induced breast cancer in female rat |
topic | βIII-tubulin breast cancer carbonic anhydrase IX doxorubicin paclitaxel survivin |
url | https://www.mdpi.com/1422-0067/22/12/6363 |
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