Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra
In this paper, a fast and sensitive localized surface plasmon resonance (LSPR) based biosensor was developed and the optimization of gold – antibody conjugates through investigation of different parameters were performed. Gold nanoparticles (AuNPs) with a size of ~20 nm were synthesized via chemical...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Society of Nanomedicine
2018-04-01
|
| Series: | Nanomedicine Research Journal |
| Subjects: | |
| Online Access: | http://www.nanomedicine-rj.com/article_32468_8f486f9e73b93b546b96d44ea3e357e4.pdf |
| _version_ | 1811276091006910464 |
|---|---|
| author | Yasamin Davatgaran Taghipour Sharmin Kharrazi Seyed Mohammad Amini |
| author_facet | Yasamin Davatgaran Taghipour Sharmin Kharrazi Seyed Mohammad Amini |
| author_sort | Yasamin Davatgaran Taghipour |
| collection | DOAJ |
| description | In this paper, a fast and sensitive localized surface plasmon resonance (LSPR) based biosensor was developed and the optimization of gold – antibody conjugates through investigation of different parameters were performed. Gold nanoparticles (AuNPs) with a size of ~20 nm were synthesized via chemical reduction of HAuCl4 with trisodium citrate as reducing and stabilizing agent. The impacts of pH of gold colloids and antibody concentrations on conjugation of electrostatically absorbed antibodies on the AuNPs surface were evaluated. The diverse amounts of antigens were added to the selected gold – antibody conjugate and the calibration curve and limit of detection of the system were successfully obtained. The UV- Vis and DLS outputs were utilized to prove the efficiency and repeatability of the system. As a result, the designed biosensor shows a convincing LOD of 400 ng/ml of antigen. It is suggested that electrostatic absorption strategy of AuNPs with negative charge to the positively charged antibodies can be an efficient methodology. Results showed an effective LSPR system for detection of small amounts of antigen in short time as well as with high accuracy. |
| first_indexed | 2024-04-12T23:50:11Z |
| format | Article |
| id | doaj.art-6a046ece6f45456289f1983770f31bd2 |
| institution | Directory Open Access Journal |
| issn | 2476-3489 2476-7123 |
| language | English |
| last_indexed | 2024-04-12T23:50:11Z |
| publishDate | 2018-04-01 |
| publisher | Iranian Society of Nanomedicine |
| record_format | Article |
| series | Nanomedicine Research Journal |
| spelling | doaj.art-6a046ece6f45456289f1983770f31bd22022-12-22T03:11:43ZengIranian Society of NanomedicineNanomedicine Research Journal2476-34892476-71232018-04-013210210810.22034/nmrj.2018.02.00732468Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) SpectraYasamin Davatgaran Taghipour0Sharmin Kharrazi1Seyed Mohammad Amini2Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences, Tehran, IranIn this paper, a fast and sensitive localized surface plasmon resonance (LSPR) based biosensor was developed and the optimization of gold – antibody conjugates through investigation of different parameters were performed. Gold nanoparticles (AuNPs) with a size of ~20 nm were synthesized via chemical reduction of HAuCl4 with trisodium citrate as reducing and stabilizing agent. The impacts of pH of gold colloids and antibody concentrations on conjugation of electrostatically absorbed antibodies on the AuNPs surface were evaluated. The diverse amounts of antigens were added to the selected gold – antibody conjugate and the calibration curve and limit of detection of the system were successfully obtained. The UV- Vis and DLS outputs were utilized to prove the efficiency and repeatability of the system. As a result, the designed biosensor shows a convincing LOD of 400 ng/ml of antigen. It is suggested that electrostatic absorption strategy of AuNPs with negative charge to the positively charged antibodies can be an efficient methodology. Results showed an effective LSPR system for detection of small amounts of antigen in short time as well as with high accuracy.http://www.nanomedicine-rj.com/article_32468_8f486f9e73b93b546b96d44ea3e357e4.pdfbiosensorsgold nanoparticleslocalized surface plasmon resonance |
| spellingShingle | Yasamin Davatgaran Taghipour Sharmin Kharrazi Seyed Mohammad Amini Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra Nanomedicine Research Journal biosensors gold nanoparticles localized surface plasmon resonance |
| title | Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra |
| title_full | Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra |
| title_fullStr | Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra |
| title_full_unstemmed | Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra |
| title_short | Antibody Conjugated Gold Nanoparticles for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra |
| title_sort | antibody conjugated gold nanoparticles for detection of small amounts of antigen based on surface plasmon resonance spr spectra |
| topic | biosensors gold nanoparticles localized surface plasmon resonance |
| url | http://www.nanomedicine-rj.com/article_32468_8f486f9e73b93b546b96d44ea3e357e4.pdf |
| work_keys_str_mv | AT yasamindavatgarantaghipour antibodyconjugatedgoldnanoparticlesfordetectionofsmallamountsofantigenbasedonsurfaceplasmonresonancesprspectra AT sharminkharrazi antibodyconjugatedgoldnanoparticlesfordetectionofsmallamountsofantigenbasedonsurfaceplasmonresonancesprspectra AT seyedmohammadamini antibodyconjugatedgoldnanoparticlesfordetectionofsmallamountsofantigenbasedonsurfaceplasmonresonancesprspectra |