Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses
To map the genomic regions and control chlorophyll fluorescence attributes under normal, salinity-, and drought-stress conditions in barley (<i>Hordeum vulgare</i> L.) at the seedling stage, an experiment was conducted in 2019–2020 using 106 F8 lines resulting from the cross between Badi...
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MDPI AG
2023-10-01
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| author | Somayyeh Makhtoum Hossein Sabouri Abdollatif Gholizadeh Leila Ahangar Mahnaz Katouzi Andrea Mastinu |
| author_facet | Somayyeh Makhtoum Hossein Sabouri Abdollatif Gholizadeh Leila Ahangar Mahnaz Katouzi Andrea Mastinu |
| author_sort | Somayyeh Makhtoum |
| collection | DOAJ |
| description | To map the genomic regions and control chlorophyll fluorescence attributes under normal, salinity-, and drought-stress conditions in barley (<i>Hordeum vulgare</i> L.) at the seedling stage, an experiment was conducted in 2019–2020 using 106 F8 lines resulting from the cross between Badia × Kavir. Initially, the different chlorophyll fluorescence parameters were evaluated. Under drought stress, the highest decrease was related to REo/CSm (59.56%), and the highest increase was related to dV/dto (77.17%). Also, under salinity stress, the highest decrease was related to Fv/Fo (59.56%), and the highest increase was related to DIo/RC (77.17%). Linkage maps were prepared using 152 SSR polymorphic markers, 72 ISSR alleles, 7 IRAP alleles, 29 CAAT alleles, 27 Scot alleles, and 15 iPBS alleles. The obtained map accounted for 999.2 centi-Morgans (cM) of the barley genome length (92% of the whole barley genome). The results indicated the importance of chromosomes 3, 2, and 7 in controlling ABS/CSm, Area, ETo/CSm, Fm, Fv, and ETo/RC under drought stress. qEToRCD-7, as a major QTL, controlled 18.3% of ETo/RC phenotypic variation under drought stress. Under salinity stress, the regions of chromosomes 2 and 7 (102 cM and 126 cM) controlled the parameters ABS/CSo, Fm, Fo, Fv, TRo/SCo, Area, ETo/CSm, and ETo/CSo. The results showed that chlorophyll fluorescence is an important parameter in the study of drought and salinity effects on barley. This is the first report of the investigation of changes in the genetic structure of quantitative genes controlling the fluorescence parameters associated with barley response to drought and salinity stresses in the Iranian barley RILs population. According to the obtained results, it is possible to use HVPLASC1B and EBmac0713 in normal conditions, ISSR21-2 and ISSR30-4 in drought conditions, and Bmac0047, Scot5-B, CAAT6-C, and ISSR30iPBS2076-4 in saline stress conditions to select genotypes with higher photosynthetic capacity in marker-assisted selection programs. |
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| spelling | doaj.art-6a064a948b3f4f6f8f45d9ac660909ed2023-11-19T14:55:28ZengMDPI AGPlants2223-77472023-10-011219351510.3390/plants12193515Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt StressesSomayyeh Makhtoum0Hossein Sabouri1Abdollatif Gholizadeh2Leila Ahangar3Mahnaz Katouzi4Andrea Mastinu5Department of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, P.O. Box 163, Gonbad 49717-99151, IranDepartment of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, P.O. Box 163, Gonbad 49717-99151, IranDepartment of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, P.O. Box 163, Gonbad 49717-99151, IranDepartment of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, P.O. Box 163, Gonbad 49717-99151, IranDepartment of Plant Breeding and Genetic Resource, Agroscope, 1260 Nyon, SwitzerlandDepartment of Molecular and Translational Medicine, Division of Pharmacology, University of Brescia, Viale Europa 11, 25123 Brescia, ItalyTo map the genomic regions and control chlorophyll fluorescence attributes under normal, salinity-, and drought-stress conditions in barley (<i>Hordeum vulgare</i> L.) at the seedling stage, an experiment was conducted in 2019–2020 using 106 F8 lines resulting from the cross between Badia × Kavir. Initially, the different chlorophyll fluorescence parameters were evaluated. Under drought stress, the highest decrease was related to REo/CSm (59.56%), and the highest increase was related to dV/dto (77.17%). Also, under salinity stress, the highest decrease was related to Fv/Fo (59.56%), and the highest increase was related to DIo/RC (77.17%). Linkage maps were prepared using 152 SSR polymorphic markers, 72 ISSR alleles, 7 IRAP alleles, 29 CAAT alleles, 27 Scot alleles, and 15 iPBS alleles. The obtained map accounted for 999.2 centi-Morgans (cM) of the barley genome length (92% of the whole barley genome). The results indicated the importance of chromosomes 3, 2, and 7 in controlling ABS/CSm, Area, ETo/CSm, Fm, Fv, and ETo/RC under drought stress. qEToRCD-7, as a major QTL, controlled 18.3% of ETo/RC phenotypic variation under drought stress. Under salinity stress, the regions of chromosomes 2 and 7 (102 cM and 126 cM) controlled the parameters ABS/CSo, Fm, Fo, Fv, TRo/SCo, Area, ETo/CSm, and ETo/CSo. The results showed that chlorophyll fluorescence is an important parameter in the study of drought and salinity effects on barley. This is the first report of the investigation of changes in the genetic structure of quantitative genes controlling the fluorescence parameters associated with barley response to drought and salinity stresses in the Iranian barley RILs population. According to the obtained results, it is possible to use HVPLASC1B and EBmac0713 in normal conditions, ISSR21-2 and ISSR30-4 in drought conditions, and Bmac0047, Scot5-B, CAAT6-C, and ISSR30iPBS2076-4 in saline stress conditions to select genotypes with higher photosynthetic capacity in marker-assisted selection programs.https://www.mdpi.com/2223-7747/12/19/3515barleychlorophyll fluorescencesalinitydroughtmolecular markersquantitative trait loci |
| spellingShingle | Somayyeh Makhtoum Hossein Sabouri Abdollatif Gholizadeh Leila Ahangar Mahnaz Katouzi Andrea Mastinu Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses Plants barley chlorophyll fluorescence salinity drought molecular markers quantitative trait loci |
| title | Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses |
| title_full | Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses |
| title_fullStr | Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses |
| title_full_unstemmed | Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses |
| title_short | Genomics and Physiology of Chlorophyll Fluorescence Parameters in <i>Hordeum vulgare</i> L. under Drought and Salt Stresses |
| title_sort | genomics and physiology of chlorophyll fluorescence parameters in i hordeum vulgare i l under drought and salt stresses |
| topic | barley chlorophyll fluorescence salinity drought molecular markers quantitative trait loci |
| url | https://www.mdpi.com/2223-7747/12/19/3515 |
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