Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments
Background and Objective: Nosocomial infections are one of main causative agents of mortality among hospital patients. This study was done for the determination of efficacy of commercial disinfectants such as: formaldehyde, glutaraldehyde, microten, the alcohol 70 and savlon–alcohol on isolated sapr...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2012-12-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-628&slc_lang=en&sid=1 |
| _version_ | 1811322104802443264 |
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| author | Nowrozi H (PhD) Kazemi A (PhD) Afshar S (MD) Adimi P (PhD) |
| author_facet | Nowrozi H (PhD) Kazemi A (PhD) Afshar S (MD) Adimi P (PhD) |
| author_sort | Nowrozi H (PhD) |
| collection | DOAJ |
| description | Background and Objective: Nosocomial infections are one of main causative agents of mortality among hospital patients. This study was done for the determination of efficacy of commercial disinfectants such as: formaldehyde, glutaraldehyde, microten, the alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments. Materials and Methods: This descriptive study was done on 33 isolated fungi from teaching hospitals of Tehran during 2009-10. The identified samples were randomly chosen. Stock fungal suspensions were supplied from each fungus with cells ranging 0.5-5×104 micro g/cfu in 1ml with spectrophotometer at the wavelength of 530 nm. For evaluation of antifungal activity of commercial disinfectants formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol disinfectants, 0.25cc stock solutions were mixed with 3.75 cc disinfectants solutions and the new diluted samples held at 25c for 15, 30 and 60 min. The culture medium was checked for growth of fungi until 8 weeks. Results: Following specific period isolated fungi were including Aspergillus spp with 39.4%, Penicillium spp with 36.4%, Fusarium spp with 12.1%, Rhizopus with 6.1%, Alternaria and Circinella with 3 %. Formaldehyde 8% and glutaraldehyde 8% with activity against 63.6% and 39.3% were effective disinfectants at 15 min. Formaldehyde 8% with activity against 74.8% of fungi, was effective disinfectant at 30 min. Glutaraldehyde 8% and formaldehyde 8% with 100% prevention of growth were effective disinfectants at 60 min. Conclusion: According to this study formaldehyde 8% and glutaraldehyde 8% showed to have the highest antifungal activities. Synergetic fungicidal activity of comenercial disinfectants, dependent on time and concentration. |
| first_indexed | 2024-04-13T13:29:17Z |
| format | Article |
| id | doaj.art-6a08cc57dc5a4f2b93b5f7cbb9a21344 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-04-13T13:29:17Z |
| publishDate | 2012-12-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-6a08cc57dc5a4f2b93b5f7cbb9a213442022-12-22T02:45:02ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802012-12-01144107112Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environmentsNowrozi H (PhD)0Kazemi A (PhD)1Afshar S (MD)2Adimi P (PhD)3 Assistant Professor, Department of Medical Mycology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Assistant Professor, Department of Pharmacology, Young Researchers Club, Islamic Azad University Science and Research Branch, Tehran, Iran Pharmacologist Assistant Professor, Department of Medical Mycology and parasitology, Islamic Azad University Medical Branch, Tehran, Iran Background and Objective: Nosocomial infections are one of main causative agents of mortality among hospital patients. This study was done for the determination of efficacy of commercial disinfectants such as: formaldehyde, glutaraldehyde, microten, the alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments. Materials and Methods: This descriptive study was done on 33 isolated fungi from teaching hospitals of Tehran during 2009-10. The identified samples were randomly chosen. Stock fungal suspensions were supplied from each fungus with cells ranging 0.5-5×104 micro g/cfu in 1ml with spectrophotometer at the wavelength of 530 nm. For evaluation of antifungal activity of commercial disinfectants formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol disinfectants, 0.25cc stock solutions were mixed with 3.75 cc disinfectants solutions and the new diluted samples held at 25c for 15, 30 and 60 min. The culture medium was checked for growth of fungi until 8 weeks. Results: Following specific period isolated fungi were including Aspergillus spp with 39.4%, Penicillium spp with 36.4%, Fusarium spp with 12.1%, Rhizopus with 6.1%, Alternaria and Circinella with 3 %. Formaldehyde 8% and glutaraldehyde 8% with activity against 63.6% and 39.3% were effective disinfectants at 15 min. Formaldehyde 8% with activity against 74.8% of fungi, was effective disinfectant at 30 min. Glutaraldehyde 8% and formaldehyde 8% with 100% prevention of growth were effective disinfectants at 60 min. Conclusion: According to this study formaldehyde 8% and glutaraldehyde 8% showed to have the highest antifungal activities. Synergetic fungicidal activity of comenercial disinfectants, dependent on time and concentration.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-628&slc_lang=en&sid=1DisinfectantFormaldehydeGlutaraldehydeMicrotenAlcohol 70Savlon–alcoholsaprophytic fungi |
| spellingShingle | Nowrozi H (PhD) Kazemi A (PhD) Afshar S (MD) Adimi P (PhD) Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments مجله دانشگاه علوم پزشکی گرگان Disinfectant Formaldehyde Glutaraldehyde Microten Alcohol 70 Savlon–alcohol saprophytic fungi |
| title | Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments |
| title_full | Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments |
| title_fullStr | Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments |
| title_full_unstemmed | Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments |
| title_short | Antifungal activity of commercial disinfectants: formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon–alcohol on isolated saprophytic fungi from hospital environments |
| title_sort | antifungal activity of commercial disinfectants formaldehyde glutaraldehyde microten alcohol 70 and savlon alcohol on isolated saprophytic fungi from hospital environments |
| topic | Disinfectant Formaldehyde Glutaraldehyde Microten Alcohol 70 Savlon–alcohol saprophytic fungi |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-628&slc_lang=en&sid=1 |
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