Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment
Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausa...
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University of Brawijaya
2019-10-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1148 |
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| author | Teguh Suprihatin Sri Widyarti Muhaimin Rifa'i Sri Rahayu |
| author_facet | Teguh Suprihatin Sri Widyarti Muhaimin Rifa'i Sri Rahayu |
| author_sort | Teguh Suprihatin |
| collection | DOAJ |
| description | Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 ± 0.26 ρg/mL) and at day 28 (4.01 ± 0.26 ρg/mL). |
| first_indexed | 2024-03-11T20:42:22Z |
| format | Article |
| id | doaj.art-6a0909937d6740f0b1c33d92c3d34249 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-11T20:42:22Z |
| publishDate | 2019-10-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-6a0909937d6740f0b1c33d92c3d342492023-10-02T01:12:49ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172019-10-0193237242309Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) TreatmentTeguh Suprihatin0Sri Widyarti1Muhaimin Rifa'i2Sri Rahayu3Biology Department, Faculty of Mathematics and Natural Sciences, Diponegoro UniversityBiology Department, Faculty of Science and Mathematics, Brawijaya UniversityBiology Department, Faculty of Mathematics and Natural Sciences, Brawijaya UniversityBiology Department, Brawijaya UniversityPremenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 ± 0.26 ρg/mL) and at day 28 (4.01 ± 0.26 ρg/mL).https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1148menopause, turmeric powder, curcumin, malondialdehyde, estradiol |
| spellingShingle | Teguh Suprihatin Sri Widyarti Muhaimin Rifa'i Sri Rahayu Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Journal of Tropical Life Science menopause, turmeric powder, curcumin, malondialdehyde, estradiol |
| title | Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment |
| title_full | Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment |
| title_fullStr | Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment |
| title_full_unstemmed | Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment |
| title_short | Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment |
| title_sort | malondialdehyde mda ovary and estradiol blood serum levels of premenopause white rat rattus norvegicus after turmeric powder curcuma longa l treatment |
| topic | menopause, turmeric powder, curcumin, malondialdehyde, estradiol |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1148 |
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