Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma
Abstract Background Mesenchymal stem/stromal cells (MSCs) have been acknowledged as the most important stromal cells in the bone marrow (BM) microenvironment for physiologic hematopoiesis and the concomitant hematologic malignancies. However, the systematic and detailed dissection of the biological...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-024-03308-2 |
| _version_ | 1827300855999627264 |
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| author | Yu Lu Chaohui Zheng Wenxia Zhang Xuan Liu Ziwei Zhou Zhenzhen Wang Huan Hua Zhengrong Song Xuejun Zhang Shuyi Liu Leisheng Zhang Fuxu Wang |
| author_facet | Yu Lu Chaohui Zheng Wenxia Zhang Xuan Liu Ziwei Zhou Zhenzhen Wang Huan Hua Zhengrong Song Xuejun Zhang Shuyi Liu Leisheng Zhang Fuxu Wang |
| author_sort | Yu Lu |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stem/stromal cells (MSCs) have been acknowledged as the most important stromal cells in the bone marrow (BM) microenvironment for physiologic hematopoiesis and the concomitant hematologic malignancies. However, the systematic and detailed dissection of the biological and transcriptomic signatures of BM-MSCs in multiple myeloma (MM) are largely unknown. Methods In this study, we isolated and identified BM-MSCs from 10 primary MM patients and 10 healthy donors (HD). On the one hand, we compared the multifaceted biological characteristics of the indicated two BM-MSCs, including biomarker expression pattern, multilineage differentiation potential, stemness and karyotyping, together with the cellular vitality and immunosuppressive property. On the other hand, we took advantage of RNA-SEQ and bioinformatics analysis to verify the similarities and differences at the transcriptomic level between MM-MSCs and HD-MSCs. Results As to biological phenotypes and biofunctions, MM-MSCs revealed conservation in immunophenotype, stemness and differentiation towards adipocytes and chondrocytes with HD-MSCs, whereas with impaired osteogenic differentiation potential, cellular vitality and immunosuppressive property. As to transcriptomic properties, MM-MSCs revealed multidimensional alterations in gene expression profiling and genetic variations. Conclusions Overall, our date systematic and detailed reflected the multifaceted similarities and variations between MM-MSCs and HD-MSCs both at the cellular and molecular levels, and in particular, the alterations of immunomodulation and cellular viability of MM-MSCs, which wound benefit the further exploration of the pathogenesis and new drug application (NDA) of multiple myeloma from the view of BM-MSCs. |
| first_indexed | 2024-04-24T16:13:17Z |
| format | Article |
| id | doaj.art-6a09c832414e4a1897b0d796b120f689 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-24T16:13:17Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-6a09c832414e4a1897b0d796b120f6892024-03-31T11:36:07ZengBMCCancer Cell International1475-28672024-03-0124111310.1186/s12935-024-03308-2Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myelomaYu Lu0Chaohui Zheng1Wenxia Zhang2Xuan Liu3Ziwei Zhou4Zhenzhen Wang5Huan Hua6Zhengrong Song7Xuejun Zhang8Shuyi Liu9Leisheng Zhang10Fuxu Wang11Department of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Otolaryngology, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityScience and Technology Innovation Center, The Fourth People’s Hospital of Jinan & The Teaching Hospital of Shandong First Medical UniversityDepartment of Hematology, Hebei Key Laboratory of Hematology, The Second Hospital of Hebei Medical UniversityAbstract Background Mesenchymal stem/stromal cells (MSCs) have been acknowledged as the most important stromal cells in the bone marrow (BM) microenvironment for physiologic hematopoiesis and the concomitant hematologic malignancies. However, the systematic and detailed dissection of the biological and transcriptomic signatures of BM-MSCs in multiple myeloma (MM) are largely unknown. Methods In this study, we isolated and identified BM-MSCs from 10 primary MM patients and 10 healthy donors (HD). On the one hand, we compared the multifaceted biological characteristics of the indicated two BM-MSCs, including biomarker expression pattern, multilineage differentiation potential, stemness and karyotyping, together with the cellular vitality and immunosuppressive property. On the other hand, we took advantage of RNA-SEQ and bioinformatics analysis to verify the similarities and differences at the transcriptomic level between MM-MSCs and HD-MSCs. Results As to biological phenotypes and biofunctions, MM-MSCs revealed conservation in immunophenotype, stemness and differentiation towards adipocytes and chondrocytes with HD-MSCs, whereas with impaired osteogenic differentiation potential, cellular vitality and immunosuppressive property. As to transcriptomic properties, MM-MSCs revealed multidimensional alterations in gene expression profiling and genetic variations. Conclusions Overall, our date systematic and detailed reflected the multifaceted similarities and variations between MM-MSCs and HD-MSCs both at the cellular and molecular levels, and in particular, the alterations of immunomodulation and cellular viability of MM-MSCs, which wound benefit the further exploration of the pathogenesis and new drug application (NDA) of multiple myeloma from the view of BM-MSCs.https://doi.org/10.1186/s12935-024-03308-2Multiple myeloma (MM)BM-MSCsBiological signaturesTranscriptomic variationCellular viability |
| spellingShingle | Yu Lu Chaohui Zheng Wenxia Zhang Xuan Liu Ziwei Zhou Zhenzhen Wang Huan Hua Zhengrong Song Xuejun Zhang Shuyi Liu Leisheng Zhang Fuxu Wang Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma Cancer Cell International Multiple myeloma (MM) BM-MSCs Biological signatures Transcriptomic variation Cellular viability |
| title | Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma |
| title_full | Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma |
| title_fullStr | Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma |
| title_full_unstemmed | Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma |
| title_short | Characterization of the biological and transcriptomic landscapes of bone marrow-derived mesenchymal stem cells in patients with multiple myeloma |
| title_sort | characterization of the biological and transcriptomic landscapes of bone marrow derived mesenchymal stem cells in patients with multiple myeloma |
| topic | Multiple myeloma (MM) BM-MSCs Biological signatures Transcriptomic variation Cellular viability |
| url | https://doi.org/10.1186/s12935-024-03308-2 |
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