Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects
Abstract Background Diabetes mellitus deteriorates the destruction and impairs the healing of periodontal wounds and craniofacial defects. This study is to evaluate the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel...
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BMC
2023-02-01
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Series: | Stem Cell Research & Therapy |
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Online Access: | https://doi.org/10.1186/s13287-023-03238-2 |
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author | Che-Chang Tu Nai-Chen Cheng Jiashing Yu Yi-Xuan Pan Wei-Chiu Tai Yin-Chuan Chen Po-Chun Chang |
author_facet | Che-Chang Tu Nai-Chen Cheng Jiashing Yu Yi-Xuan Pan Wei-Chiu Tai Yin-Chuan Chen Po-Chun Chang |
author_sort | Che-Chang Tu |
collection | DOAJ |
description | Abstract Background Diabetes mellitus deteriorates the destruction and impairs the healing of periodontal wounds and craniofacial defects. This study is to evaluate the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. Methods Human adipose-derived stem cells (ADSCs) were isolated by lipoaspiration, pluripotent genes and trilineage differentiation were examined, and the maintenance of ADsp properties in mTG was verified. Oral mucosal wounds and calvarial osseous defects were created in diabetic rats. Gross observation, histologic evaluation, and immunohistochemistry for proliferating cells and keratinization were conducted in the mucosal wounds within 4–28 days. Micro-CT imaging, histologic evaluation, and immunohistochemistry for proliferating cells and osteogenic differentiation were conducted in the osseous defects at 7 and 28 days. Results ADSCs expressed pluripotent genes and were capable of trilineage differentiation. ADsp retained morphology and stemness in mTG. In diabetic mucosal wounds, wound closure, epithelization, and keratinization were accelerated in those with ADsp and ADsp-mTG. In diabetic osseous defects, osteogenic differentiation markers were evidently expressed, cell proliferation was promoted from day 7, and bone formation was significantly promoted at day 28 in those with osteogenically pretreated ADsp-mTG. Conclusions ADsp-mTG accelerated diabetic oral mucosal wound healing, and osteogenically pretreated ADsp-mTG promoted diabetic osseous defect regeneration, proving that ADsp-mTG facilitated diabetic periodontal wound healing and craniofacial osseous defect regeneration. |
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spelling | doaj.art-6a0d401cd5cc4229976a9dacd4df72bf2023-02-05T12:06:09ZengBMCStem Cell Research & Therapy1757-65122023-02-0114111410.1186/s13287-023-03238-2Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defectsChe-Chang Tu0Nai-Chen Cheng1Jiashing Yu2Yi-Xuan Pan3Wei-Chiu Tai4Yin-Chuan Chen5Po-Chun Chang6Graduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan UniversityDepartment of Surgery, National Taiwan University Hospital and College of MedicineDepartment of Chemical Engineering, College of Engineering, National Taiwan UniversityDivision of Periodontics, Department of Dentistry, National Taiwan University HospitalGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan UniversityDepartment of Chemical Engineering, College of Engineering, National Taiwan UniversityGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan UniversityAbstract Background Diabetes mellitus deteriorates the destruction and impairs the healing of periodontal wounds and craniofacial defects. This study is to evaluate the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. Methods Human adipose-derived stem cells (ADSCs) were isolated by lipoaspiration, pluripotent genes and trilineage differentiation were examined, and the maintenance of ADsp properties in mTG was verified. Oral mucosal wounds and calvarial osseous defects were created in diabetic rats. Gross observation, histologic evaluation, and immunohistochemistry for proliferating cells and keratinization were conducted in the mucosal wounds within 4–28 days. Micro-CT imaging, histologic evaluation, and immunohistochemistry for proliferating cells and osteogenic differentiation were conducted in the osseous defects at 7 and 28 days. Results ADSCs expressed pluripotent genes and were capable of trilineage differentiation. ADsp retained morphology and stemness in mTG. In diabetic mucosal wounds, wound closure, epithelization, and keratinization were accelerated in those with ADsp and ADsp-mTG. In diabetic osseous defects, osteogenic differentiation markers were evidently expressed, cell proliferation was promoted from day 7, and bone formation was significantly promoted at day 28 in those with osteogenically pretreated ADsp-mTG. Conclusions ADsp-mTG accelerated diabetic oral mucosal wound healing, and osteogenically pretreated ADsp-mTG promoted diabetic osseous defect regeneration, proving that ADsp-mTG facilitated diabetic periodontal wound healing and craniofacial osseous defect regeneration.https://doi.org/10.1186/s13287-023-03238-2Mesenchymal stem cellDiabetesWound healingTissue engineeringBone regenerationHydrogels |
spellingShingle | Che-Chang Tu Nai-Chen Cheng Jiashing Yu Yi-Xuan Pan Wei-Chiu Tai Yin-Chuan Chen Po-Chun Chang Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects Stem Cell Research & Therapy Mesenchymal stem cell Diabetes Wound healing Tissue engineering Bone regeneration Hydrogels |
title | Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
title_full | Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
title_fullStr | Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
title_full_unstemmed | Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
title_short | Adipose-derived stem cell spheroid-laden microbial transglutaminase cross-linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
title_sort | adipose derived stem cell spheroid laden microbial transglutaminase cross linked gelatin hydrogel for treating diabetic periodontal wounds and craniofacial defects |
topic | Mesenchymal stem cell Diabetes Wound healing Tissue engineering Bone regeneration Hydrogels |
url | https://doi.org/10.1186/s13287-023-03238-2 |
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