Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance
Aim: The present study was conducted to determine the zoonotic importance of methicillin resistant staphylococci associated with bovine mastitis and their potential role in transmission to animal handlers. Materials and Methods: A total of 158 milk samples from bovine mastitis cases and 126 nasal s...
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| Format: | Article |
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Veterinary World
2014-06-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.7/June-2014/12.pdf |
| _version_ | 1830356778141876224 |
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| author | S. Vishnupriya P. X. Antony H. K. Mukhopadhyay R. M. Pillai J. Thanislass V. M. Vivek Srinivas R. Sumanth Kumar |
| author_facet | S. Vishnupriya P. X. Antony H. K. Mukhopadhyay R. M. Pillai J. Thanislass V. M. Vivek Srinivas R. Sumanth Kumar |
| author_sort | S. Vishnupriya |
| collection | DOAJ |
| description | Aim: The present study was conducted to determine the zoonotic importance of methicillin resistant staphylococci associated with bovine mastitis and their potential role in transmission to animal handlers.
Materials and Methods: A total of 158 milk samples from bovine mastitis cases and 126 nasal swabs from the animal handlers were sampled in and around Pondicherry (Southern India). The Presence of Staphylococcal organism was confirmed by PCR amplification using the genus specific primers and among the isolated Staphylococci; methicillin resistance was identified by genetic amplification of mec A methicillin resistant gene. Then the amplified gene from the bacteria expressing the mecA gene (PBP2a) (~2kb fragment) was further sequenced using four sets of primer pairs and aligned for determining their genetic relatedness between the sequences. Both phenotypic and genotypic analysis was carried out for the six MRS isolates (three bovine and three human) in this study.
Results: Out of 158 mastitis milk samples; 96 and 19 bovine isolates were found to be positive for Staphylococcal genus specific PCR and methicillin resistant (mecA) gene PCR, respectively. Similarly, Out of 126 human nasal swabs, 64 and 13 human isolates were found to be positive for Staphylococcal genus specific PCR and mec A gene PCR, respectively. Among the 160 staphylococcal isolates (Bovine and Human origin); 51 were identified as coagulase-positive staphylococci (CPS) and remaining as coagulase-negative staphylococci (CONS). The results obtained in this study revealed the presence of many species of Staphylococci but the predominant species were Staphylococcus aureus and S. epidermidis. The Sequence analysis of the mec A gene of human isolates obtained in this study had a maximum identity (99% -100%) with the bovine isolates.
Conclusion: The phenotypic and genotypic analysis carried out for the six MRS (Methicillin Resistant Staphylococci) isolates in this study were indistinguishable and epidemiologically related, which may indicate the transmission of MRS between bovine and humans. The occurrence of methicillin resistance among staphylococci isolated from cases of bovine mastitis is increasing, necessitating the periodic surveillance for antimicrobial resistance patterns of Staphylococci in order to control the spread of MRS. |
| first_indexed | 2024-12-20T02:12:11Z |
| format | Article |
| id | doaj.art-6a0fbd96386d4ec78c48dd1130ec5629 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-20T02:12:11Z |
| publishDate | 2014-06-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-6a0fbd96386d4ec78c48dd1130ec56292022-12-21T19:57:02ZengVeterinary WorldVeterinary World0972-89882231-09162014-06-017642242710.14202/vetworld.2014.422-427Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importanceS. Vishnupriya0P. X. Antony1H. K. Mukhopadhyay2R. M. Pillai3J. Thanislass4V. M. Vivek Srinivas 5R. Sumanth Kumar6Department of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; vishnupriyavet@gmail.comDepartment of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; pxantony@gmail.comDepartment of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; hkmparvo@gmail.com Department of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; drrmadhu@sify.comDepartment of Veterinary Biochemistry, Rajiv Gandhi College of Veterinary and Animal Sciences, 605 009, India; jthanislass@gmail.com Department of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; vivekvet24@gmail.com Department of Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry University, Pondicherry - 605 009, India; sumanth.vetco@gmail.comAim: The present study was conducted to determine the zoonotic importance of methicillin resistant staphylococci associated with bovine mastitis and their potential role in transmission to animal handlers. Materials and Methods: A total of 158 milk samples from bovine mastitis cases and 126 nasal swabs from the animal handlers were sampled in and around Pondicherry (Southern India). The Presence of Staphylococcal organism was confirmed by PCR amplification using the genus specific primers and among the isolated Staphylococci; methicillin resistance was identified by genetic amplification of mec A methicillin resistant gene. Then the amplified gene from the bacteria expressing the mecA gene (PBP2a) (~2kb fragment) was further sequenced using four sets of primer pairs and aligned for determining their genetic relatedness between the sequences. Both phenotypic and genotypic analysis was carried out for the six MRS isolates (three bovine and three human) in this study. Results: Out of 158 mastitis milk samples; 96 and 19 bovine isolates were found to be positive for Staphylococcal genus specific PCR and methicillin resistant (mecA) gene PCR, respectively. Similarly, Out of 126 human nasal swabs, 64 and 13 human isolates were found to be positive for Staphylococcal genus specific PCR and mec A gene PCR, respectively. Among the 160 staphylococcal isolates (Bovine and Human origin); 51 were identified as coagulase-positive staphylococci (CPS) and remaining as coagulase-negative staphylococci (CONS). The results obtained in this study revealed the presence of many species of Staphylococci but the predominant species were Staphylococcus aureus and S. epidermidis. The Sequence analysis of the mec A gene of human isolates obtained in this study had a maximum identity (99% -100%) with the bovine isolates. Conclusion: The phenotypic and genotypic analysis carried out for the six MRS (Methicillin Resistant Staphylococci) isolates in this study were indistinguishable and epidemiologically related, which may indicate the transmission of MRS between bovine and humans. The occurrence of methicillin resistance among staphylococci isolated from cases of bovine mastitis is increasing, necessitating the periodic surveillance for antimicrobial resistance patterns of Staphylococci in order to control the spread of MRS.http://www.veterinaryworld.org/Vol.7/June-2014/12.pdfbovine mastitiscoagulase-negative staphylococci (CONS)methicillin resistant Staphylococcus aureus (MRSA)sequence analysis |
| spellingShingle | S. Vishnupriya P. X. Antony H. K. Mukhopadhyay R. M. Pillai J. Thanislass V. M. Vivek Srinivas R. Sumanth Kumar Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance Veterinary World bovine mastitis coagulase-negative staphylococci (CONS) methicillin resistant Staphylococcus aureus (MRSA) sequence analysis |
| title | Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| title_full | Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| title_fullStr | Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| title_full_unstemmed | Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| title_short | Methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| title_sort | methicillin resistant staphylococci associated with bovine mastitis and their zoonotic importance |
| topic | bovine mastitis coagulase-negative staphylococci (CONS) methicillin resistant Staphylococcus aureus (MRSA) sequence analysis |
| url | http://www.veterinaryworld.org/Vol.7/June-2014/12.pdf |
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