Streptomyces as Microbial Chassis for Heterologous Protein Expression
Heterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of exp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.804295/full |
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| author | Soonkyu Hwang Soonkyu Hwang Yongjae Lee Yongjae Lee Ji Hun Kim Ji Hun Kim Gahyeon Kim Gahyeon Kim Hyeseong Kim Hyeseong Kim Woori Kim Woori Kim Suhyung Cho Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho Byung-Kwan Cho |
| author_facet | Soonkyu Hwang Soonkyu Hwang Yongjae Lee Yongjae Lee Ji Hun Kim Ji Hun Kim Gahyeon Kim Gahyeon Kim Hyeseong Kim Hyeseong Kim Woori Kim Woori Kim Suhyung Cho Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho Byung-Kwan Cho |
| author_sort | Soonkyu Hwang |
| collection | DOAJ |
| description | Heterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of expressing various secondary metabolite biosynthetic gene clusters and secretory enzymes. However, there are several issues that limit their use, including low yield, difficulty in genetic manipulation, and their complex cellular features. In this review, we summarize rational engineering approaches to optimizing the heterologous production of secondary metabolites and recombinant proteins in Streptomyces species in terms of genetic tool development and chassis construction. Further perspectives on the development of optimal Streptomyces chassis by the design-build-test-learn cycle in systems are suggested, which may increase the availability of secondary metabolites and recombinant proteins. |
| first_indexed | 2024-12-19T12:26:31Z |
| format | Article |
| id | doaj.art-6a11045c3a3c4faba88313858c18f466 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-19T12:26:31Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-6a11045c3a3c4faba88313858c18f4662022-12-21T20:21:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-12-01910.3389/fbioe.2021.804295804295Streptomyces as Microbial Chassis for Heterologous Protein ExpressionSoonkyu Hwang0Soonkyu Hwang1Yongjae Lee2Yongjae Lee3Ji Hun Kim4Ji Hun Kim5Gahyeon Kim6Gahyeon Kim7Hyeseong Kim8Hyeseong Kim9Woori Kim10Woori Kim11Suhyung Cho12Suhyung Cho13Bernhard O. Palsson14Bernhard O. Palsson15Bernhard O. Palsson16Byung-Kwan Cho17Byung-Kwan Cho18Byung-Kwan Cho19Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, United StatesDepartment of Pediatrics, University of California, San Diego, La Jolla, CA, United StatesNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, DenmarkDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaKAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaInnovative Biomaterials Research Center, KAIST Institutes, Korea Advanced Institute of Science and Technology, Daejeon, South KoreaHeterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of expressing various secondary metabolite biosynthetic gene clusters and secretory enzymes. However, there are several issues that limit their use, including low yield, difficulty in genetic manipulation, and their complex cellular features. In this review, we summarize rational engineering approaches to optimizing the heterologous production of secondary metabolites and recombinant proteins in Streptomyces species in terms of genetic tool development and chassis construction. Further perspectives on the development of optimal Streptomyces chassis by the design-build-test-learn cycle in systems are suggested, which may increase the availability of secondary metabolites and recombinant proteins.https://www.frontiersin.org/articles/10.3389/fbioe.2021.804295/fullstreptomycesheterologous expressionchassisrecombinant proteinsecondary metabolite |
| spellingShingle | Soonkyu Hwang Soonkyu Hwang Yongjae Lee Yongjae Lee Ji Hun Kim Ji Hun Kim Gahyeon Kim Gahyeon Kim Hyeseong Kim Hyeseong Kim Woori Kim Woori Kim Suhyung Cho Suhyung Cho Bernhard O. Palsson Bernhard O. Palsson Bernhard O. Palsson Byung-Kwan Cho Byung-Kwan Cho Byung-Kwan Cho Streptomyces as Microbial Chassis for Heterologous Protein Expression Frontiers in Bioengineering and Biotechnology streptomyces heterologous expression chassis recombinant protein secondary metabolite |
| title | Streptomyces as Microbial Chassis for Heterologous Protein Expression |
| title_full | Streptomyces as Microbial Chassis for Heterologous Protein Expression |
| title_fullStr | Streptomyces as Microbial Chassis for Heterologous Protein Expression |
| title_full_unstemmed | Streptomyces as Microbial Chassis for Heterologous Protein Expression |
| title_short | Streptomyces as Microbial Chassis for Heterologous Protein Expression |
| title_sort | streptomyces as microbial chassis for heterologous protein expression |
| topic | streptomyces heterologous expression chassis recombinant protein secondary metabolite |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.804295/full |
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