Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study
ObjectiveThis study aimed to explore the relationship between the plasma metabolites of adolescent obesity and hypertension and whether metabolite alterations had a mediating effort between adolescent obesity and hypertension.MethodsWe applied untargeted ultra-performance liquid chromatography–mass...
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| Format: | Article |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1172290/full |
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| author | Zhi-Ping Wu Wei Wei Yuan Cheng Jing-Yi Chen Yang Liu Shan Liu Meng-Die Hu Heng Zhao Xiao-Feng Li Xin Chen |
| author_facet | Zhi-Ping Wu Wei Wei Yuan Cheng Jing-Yi Chen Yang Liu Shan Liu Meng-Die Hu Heng Zhao Xiao-Feng Li Xin Chen |
| author_sort | Zhi-Ping Wu |
| collection | DOAJ |
| description | ObjectiveThis study aimed to explore the relationship between the plasma metabolites of adolescent obesity and hypertension and whether metabolite alterations had a mediating effort between adolescent obesity and hypertension.MethodsWe applied untargeted ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) to detect the plasma metabolomic profiles of 105 adolescents. All participants were selected randomly based on a previous cross-sectional study. An orthogonal partial least squares- discriminant analysis (OPLS-DA), followed by univariate statistics and enrichment analysis, was used to identify differential metabolites. Using logistic regression for variable selection, an obesity-related metabolite score (OMS, OMS=∑k=1nβnmetabolite n) was constructed from the metabolites identified, and hypertension risk was estimated.ResultsIn our study, based on P< 0.05, variable importance in projection (VIP) > 1.0, and impact value > 0.1, we identified a total of 12 differential metabolites. Significantly altered metabolic pathways were the sphingolipid metabolism, purine metabolism, pyrimidine metabolism, phospholipid metabolism, steroid hormone biosynthesis, tryptophan, tyrosine, and phenylalanine biosynthesis. The logistic regression selection resulted in a four-metabolite score (thymidine, sphingomyelin (SM) d40:1, 4-hydroxyestradiol, and L-lysinamide), which was positively associated with hypertension risk (odds ratio: 7.79; 95% confidence interval: 2.13, 28.47; for the quintile 4 compared with quartile 1 of OMS) after multivariable adjustment.ConclusionsThe OMS constructed from four differential metabolites was used to predict the risk of hypertension in adolescents. These findings could provide sensitive biomarkers for the early recognition of hypertension in adolescents with obesity. |
| first_indexed | 2024-04-09T13:43:59Z |
| format | Article |
| id | doaj.art-6a11fea8ede5426c9bf5c859cba76c33 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-09T13:43:59Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-6a11fea8ede5426c9bf5c859cba76c332023-05-09T05:43:02ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-05-011410.3389/fendo.2023.11722901172290Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics studyZhi-Ping Wu0Wei Wei1Yuan Cheng2Jing-Yi Chen3Yang Liu4Shan Liu5Meng-Die Hu6Heng Zhao7Xiao-Feng Li8Xin Chen9Department of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Neurosurgery, Central Hospital of Dalian University of Technology, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaInstitute of Health Science, China Medical University, Shenyang, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaDepartment of Epidemiology, School of Public Health, Dalian Medical University, Dalian, ChinaObjectiveThis study aimed to explore the relationship between the plasma metabolites of adolescent obesity and hypertension and whether metabolite alterations had a mediating effort between adolescent obesity and hypertension.MethodsWe applied untargeted ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) to detect the plasma metabolomic profiles of 105 adolescents. All participants were selected randomly based on a previous cross-sectional study. An orthogonal partial least squares- discriminant analysis (OPLS-DA), followed by univariate statistics and enrichment analysis, was used to identify differential metabolites. Using logistic regression for variable selection, an obesity-related metabolite score (OMS, OMS=∑k=1nβnmetabolite n) was constructed from the metabolites identified, and hypertension risk was estimated.ResultsIn our study, based on P< 0.05, variable importance in projection (VIP) > 1.0, and impact value > 0.1, we identified a total of 12 differential metabolites. Significantly altered metabolic pathways were the sphingolipid metabolism, purine metabolism, pyrimidine metabolism, phospholipid metabolism, steroid hormone biosynthesis, tryptophan, tyrosine, and phenylalanine biosynthesis. The logistic regression selection resulted in a four-metabolite score (thymidine, sphingomyelin (SM) d40:1, 4-hydroxyestradiol, and L-lysinamide), which was positively associated with hypertension risk (odds ratio: 7.79; 95% confidence interval: 2.13, 28.47; for the quintile 4 compared with quartile 1 of OMS) after multivariable adjustment.ConclusionsThe OMS constructed from four differential metabolites was used to predict the risk of hypertension in adolescents. These findings could provide sensitive biomarkers for the early recognition of hypertension in adolescents with obesity.https://www.frontiersin.org/articles/10.3389/fendo.2023.1172290/fulladolescent obesityuntargeted liquid chromatography-mass spectrometry analysishypertensionsystolic blood pressurediastolic blood pressure |
| spellingShingle | Zhi-Ping Wu Wei Wei Yuan Cheng Jing-Yi Chen Yang Liu Shan Liu Meng-Die Hu Heng Zhao Xiao-Feng Li Xin Chen Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study Frontiers in Endocrinology adolescent obesity untargeted liquid chromatography-mass spectrometry analysis hypertension systolic blood pressure diastolic blood pressure |
| title | Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study |
| title_full | Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study |
| title_fullStr | Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study |
| title_full_unstemmed | Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study |
| title_short | Altered adolescents obesity metabolism is associated with hypertension: a UPLC-MS-based untargeted metabolomics study |
| title_sort | altered adolescents obesity metabolism is associated with hypertension a uplc ms based untargeted metabolomics study |
| topic | adolescent obesity untargeted liquid chromatography-mass spectrometry analysis hypertension systolic blood pressure diastolic blood pressure |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1172290/full |
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