Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology
The carcinogenesis of non–small cell lung carcinoma has been found to associate with activating and resistant mutations in the tyrosine kinase domain of specific oncogenes. Here, we assessed the type, frequency, and abundance of epithelial growth factor receptor, KRAS, BRAF, and ALK mutations in 154...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IOS Press
2017-02-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317691413 |
| _version_ | 1818610919793491968 |
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| author | Jian Shi Meng Yuan Zhan-Dong Wang Xiao-Li Xu Lei Hong Shenglin Sun |
| author_facet | Jian Shi Meng Yuan Zhan-Dong Wang Xiao-Li Xu Lei Hong Shenglin Sun |
| author_sort | Jian Shi |
| collection | DOAJ |
| description | The carcinogenesis of non–small cell lung carcinoma has been found to associate with activating and resistant mutations in the tyrosine kinase domain of specific oncogenes. Here, we assessed the type, frequency, and abundance of epithelial growth factor receptor, KRAS, BRAF, and ALK mutations in 154 non–small cell lung carcinoma specimens using single-molecule amplification and re-sequencing technology. We found that epithelial growth factor receptor mutations were the most prevalent (44.2%), followed by KRAS (18.8%), ALK (7.8%), and BRAF (5.8%) mutations. The type and abundance of the mutations in tumor specimens appeared to be heterogeneous. Thus, we conclude that identification of clinically significant oncogenic mutations may improve the classification of patients and provide valuable information for determination of the therapeutic strategies. |
| first_indexed | 2024-12-16T15:22:05Z |
| format | Article |
| id | doaj.art-6a137bb688f94592bed97ff6ef05b2d8 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-16T15:22:05Z |
| publishDate | 2017-02-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-6a137bb688f94592bed97ff6ef05b2d82022-12-21T22:26:36ZengIOS PressTumor Biology1423-03802017-02-013910.1177/1010428317691413Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technologyJian Shi0Meng Yuan1Zhan-Dong Wang2Xiao-Li Xu3Lei Hong4Shenglin Sun5Department of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaCollege of Science and Technology, China Three Gorges University, Yichang, ChinaDepartment of Pathology, Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Medical Record Library, Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaThe carcinogenesis of non–small cell lung carcinoma has been found to associate with activating and resistant mutations in the tyrosine kinase domain of specific oncogenes. Here, we assessed the type, frequency, and abundance of epithelial growth factor receptor, KRAS, BRAF, and ALK mutations in 154 non–small cell lung carcinoma specimens using single-molecule amplification and re-sequencing technology. We found that epithelial growth factor receptor mutations were the most prevalent (44.2%), followed by KRAS (18.8%), ALK (7.8%), and BRAF (5.8%) mutations. The type and abundance of the mutations in tumor specimens appeared to be heterogeneous. Thus, we conclude that identification of clinically significant oncogenic mutations may improve the classification of patients and provide valuable information for determination of the therapeutic strategies.https://doi.org/10.1177/1010428317691413 |
| spellingShingle | Jian Shi Meng Yuan Zhan-Dong Wang Xiao-Li Xu Lei Hong Shenglin Sun Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology Tumor Biology |
| title | Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology |
| title_full | Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology |
| title_fullStr | Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology |
| title_full_unstemmed | Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology |
| title_short | Comprehensive profiling and quantitation of oncogenic mutations in non–small cell lung carcinoma using single-molecule amplification and re-sequencing technology |
| title_sort | comprehensive profiling and quantitation of oncogenic mutations in non small cell lung carcinoma using single molecule amplification and re sequencing technology |
| url | https://doi.org/10.1177/1010428317691413 |
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