Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells
Background: The risk of infectious, autoimmune and immunodeficiency diseases and cancers rise in opioid addicts due to changes in innate and acquired immune responses. Three types of opioid receptors (К،δ،μ) are expressed on the surface of lymphocytes and mononuclear phagocytes. The present study wa...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2015-01-01
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| Series: | Addiction and Health |
| Subjects: | |
| Online Access: | https://ahj.kmu.ac.ir/article_84640_be596d1509da0553b170c6f556fc1627.pdf |
| _version_ | 1797681863286849536 |
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| author | Gholamreza Asadikaram Somayeh Igder Zahra Jamali Nader Shahrokhi Hamid Najafipour Mostafa Shokoohi Abdollah Jafarzadeh Mohammad Kazemi-Arababadi |
| author_facet | Gholamreza Asadikaram Somayeh Igder Zahra Jamali Nader Shahrokhi Hamid Najafipour Mostafa Shokoohi Abdollah Jafarzadeh Mohammad Kazemi-Arababadi |
| author_sort | Gholamreza Asadikaram |
| collection | DOAJ |
| description | Background: The risk of infectious, autoimmune and immunodeficiency diseases and cancers rise in opioid addicts due to changes in innate and acquired immune responses. Three types of opioid receptors (К،δ،μ) are expressed on the surface of lymphocytes and mononuclear phagocytes. The present study was designed to examine the effects of different concentrations of opium on the secretion of some cytokines produced by lymphocyte cells. Methods: Jurkat cells were exposed to different concentrations of opium for periods of 6, 24 and 72 h in cell culture medium. The amount of interleukin-6 (IL-6), interferon-γ (IFN-γ) and transforming growth factor-b (TGF-β) were then measured using enzyme-linked immunosorbent assay (ELISA) method. Findings: The results showed that opium increases the secretion of IL-6 in different concentration of opium in 6 h. The amount of IFN-γ decreased in 6 h and increased in 24 h significantly compared with control. On the other hand, opium had an inhibitory effect on the TGF-β secretion in 6, 24 and 72 h. Conclusion: Overall, the study showed that opium stimulates pro-inflammatory and suppressed anti-inflammatory cytokine secretion in Jurkat cells. This may account for the negative effect of opium on the immune system leading to chronic inflammation and a base for many disorders in opium addicts. |
| first_indexed | 2024-03-11T23:51:10Z |
| format | Article |
| id | doaj.art-6a1455fb789a4a89b9171ed4ada29905 |
| institution | Directory Open Access Journal |
| issn | 2008-4633 2008-8469 |
| language | English |
| last_indexed | 2024-03-11T23:51:10Z |
| publishDate | 2015-01-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Addiction and Health |
| spelling | doaj.art-6a1455fb789a4a89b9171ed4ada299052023-09-19T06:51:07ZengKerman University of Medical SciencesAddiction and Health2008-46332008-84692015-01-0171-2475384640Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat CellsGholamreza Asadikaram0Somayeh Igder1Zahra Jamali2Nader Shahrokhi3Hamid Najafipour4Mostafa Shokoohi5Abdollah Jafarzadeh6Mohammad Kazemi-Arababadi7Professor, Physiology Research Center, Institute of Neuropharmacology, Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, IranPhD Student, Molecular Biology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IranPhD Student, Molecular Biology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IranAssociate Professor, Physiology Research Center, Institute of Neuropharmacology, Department of Physiology, School of Medicine, Kerman University of Medical Sciences, Kerman, IranProfessor, Physiology Research Center, Institute of Neuropharmacology, Department of Physiology, School of Medicine, Kerman University of Medical Sciences, Kerman, IranResearch Center for Modeling in Health, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, IranProfessor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranAssistant Professor, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranBackground: The risk of infectious, autoimmune and immunodeficiency diseases and cancers rise in opioid addicts due to changes in innate and acquired immune responses. Three types of opioid receptors (К،δ،μ) are expressed on the surface of lymphocytes and mononuclear phagocytes. The present study was designed to examine the effects of different concentrations of opium on the secretion of some cytokines produced by lymphocyte cells. Methods: Jurkat cells were exposed to different concentrations of opium for periods of 6, 24 and 72 h in cell culture medium. The amount of interleukin-6 (IL-6), interferon-γ (IFN-γ) and transforming growth factor-b (TGF-β) were then measured using enzyme-linked immunosorbent assay (ELISA) method. Findings: The results showed that opium increases the secretion of IL-6 in different concentration of opium in 6 h. The amount of IFN-γ decreased in 6 h and increased in 24 h significantly compared with control. On the other hand, opium had an inhibitory effect on the TGF-β secretion in 6, 24 and 72 h. Conclusion: Overall, the study showed that opium stimulates pro-inflammatory and suppressed anti-inflammatory cytokine secretion in Jurkat cells. This may account for the negative effect of opium on the immune system leading to chronic inflammation and a base for many disorders in opium addicts.https://ahj.kmu.ac.ir/article_84640_be596d1509da0553b170c6f556fc1627.pdfopiumjurkat cellinterferon-γinterleukin 6transforming growth factor beta |
| spellingShingle | Gholamreza Asadikaram Somayeh Igder Zahra Jamali Nader Shahrokhi Hamid Najafipour Mostafa Shokoohi Abdollah Jafarzadeh Mohammad Kazemi-Arababadi Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells Addiction and Health opium jurkat cell interferon-γ interleukin 6 transforming growth factor beta |
| title | Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells |
| title_full | Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells |
| title_fullStr | Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells |
| title_full_unstemmed | Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells |
| title_short | Effects of Different Concentrations of Opium on the Secretion of Interleukin-6, Interferon-γ and Transforming Growth Factor Beta Cytokines from Jurkat Cells |
| title_sort | effects of different concentrations of opium on the secretion of interleukin 6 interferon γ and transforming growth factor beta cytokines from jurkat cells |
| topic | opium jurkat cell interferon-γ interleukin 6 transforming growth factor beta |
| url | https://ahj.kmu.ac.ir/article_84640_be596d1509da0553b170c6f556fc1627.pdf |
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