Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor
Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techn...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/10/845 |
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| author | Jesús Maldonado Ana Belén González-Guerrero Adrián Fernández-Gavela Juan José González-López Laura M. Lechuga |
| author_facet | Jesús Maldonado Ana Belén González-Guerrero Adrián Fernández-Gavela Juan José González-López Laura M. Lechuga |
| author_sort | Jesús Maldonado |
| collection | DOAJ |
| description | Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene <i>blaCTX-M-15</i> and the carbapenemase-encoding gene <i>blaNDM-5</i> We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria. |
| first_indexed | 2024-03-10T15:29:33Z |
| format | Article |
| id | doaj.art-6a1614313fe94de5a88d4ee6dc358c7d |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T15:29:33Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-6a1614313fe94de5a88d4ee6dc358c7d2023-11-20T17:43:04ZengMDPI AGDiagnostics2075-44182020-10-01101084510.3390/diagnostics10100845Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric BiosensorJesús Maldonado0Ana Belén González-Guerrero1Adrián Fernández-Gavela2Juan José González-López3Laura M. Lechuga4Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193 Barcelona, SpainNanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193 Barcelona, SpainNanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193 Barcelona, SpainDepartment of Clinical Microbiology, Hospital Univeritari Vall d’Hebron (HUVH), Vall d’Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, 08035 Barcelona, SpainNanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193 Barcelona, SpainInfections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene <i>blaCTX-M-15</i> and the carbapenemase-encoding gene <i>blaNDM-5</i> We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria.https://www.mdpi.com/2075-4418/10/10/845nanophotonic biosensormultidrug-resistance<i>Escherichia coli</i>bimodal waveguide interferometer<i>NDM</i><i>CTX-M</i> |
| spellingShingle | Jesús Maldonado Ana Belén González-Guerrero Adrián Fernández-Gavela Juan José González-López Laura M. Lechuga Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor Diagnostics nanophotonic biosensor multidrug-resistance <i>Escherichia coli</i> bimodal waveguide interferometer <i>NDM</i> <i>CTX-M</i> |
| title | Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor |
| title_full | Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor |
| title_fullStr | Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor |
| title_full_unstemmed | Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor |
| title_short | Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor |
| title_sort | ultrasensitive label free detection of unamplified multidrug resistance bacteria genes with a bimodal waveguide interferometric biosensor |
| topic | nanophotonic biosensor multidrug-resistance <i>Escherichia coli</i> bimodal waveguide interferometer <i>NDM</i> <i>CTX-M</i> |
| url | https://www.mdpi.com/2075-4418/10/10/845 |
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