Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig
The metabolic difference in caecal digesta between Jinhua pig and Landrace pig was compared. Twenty weaned piglets at 28 days of age, including ten Landrace pigs (a Western pig breed) and ten Jinhua pigs (a Chinese native pig breed), were randomly selected and allocated into two groups. The pigs wer...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2019-08-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201908-0002_comparative-metabolomic-analysis-of-caecal-digesta-between-jinhua-pig-and-landrace-pig.php |
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| author | Yujie Gong Xiaoting Zou Wenrui Xia Xueting Wen Xiaojun Zhang Yingping Xiao Hua Yang |
| author_facet | Yujie Gong Xiaoting Zou Wenrui Xia Xueting Wen Xiaojun Zhang Yingping Xiao Hua Yang |
| author_sort | Yujie Gong |
| collection | DOAJ |
| description | The metabolic difference in caecal digesta between Jinhua pig and Landrace pig was compared. Twenty weaned piglets at 28 days of age, including ten Landrace pigs (a Western pig breed) and ten Jinhua pigs (a Chinese native pig breed), were randomly selected and allocated into two groups. The pigs were fed the same corn-soybean diet on the same pig farm. At the age of 240 days, all pigs of each group were slaughtered, the digesta in the caecum of the twenty pigs were collected for metabolomic analysis and determination of short-chain fatty acids (SCFAs). The results showed that a total of 56 different metabolites (22 metabolites named and 34 metabolites without identification) were detected in caecal digesta using a gas chromatography time-of-flight/mass spectrometry (GC-TOF-MS)-based metabolomic approach. Forty-six of the 56 metabolites were upregulated significantly (P < 0.05) in Landrace group compared with Jinhua group. The metabolic pathways with different impact value in which different metabolites were mainly involved were tyrosine metabolism, citrate cycle and steroid biosynthesis. In addition, we found that Landrace accumulated more SCFAs in caecal digesta, while the concentrations of acetic acid (P < 0.01) and butyric acid (P < 0.05) in caecal digesta of Jinhua pig were markedly lower than those of Landrace pig. Collectively, our study was the first to compare the metabolic difference in caecal digesta between Jinhua pig and Landrace pig using a metabolomics approach, which might be used as a potential metabolomics mechanism to research different breeds of pigs. |
| first_indexed | 2024-04-10T08:25:05Z |
| format | Article |
| id | doaj.art-6a16280a5d5b44da945df7867f7d6e93 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:05Z |
| publishDate | 2019-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Czech Journal of Animal Science |
| spelling | doaj.art-6a16280a5d5b44da945df7867f7d6e932023-02-23T03:33:40ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092019-08-0164833234210.17221/43/2018-CJAScjs-201908-0002Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pigYujie Gong0Xiaoting Zou1Wenrui Xia2Xueting Wen3Xiaojun Zhang4Yingping Xiao5Hua Yang6Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou, P.R. ChinaKey Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou, P.R. ChinaKey Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou, P.R. ChinaInstitute of Quality and Standards for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. ChinaInstitute of Animal Husbandry and Veterinary Medicine, Jinhua Academy of Agricultural Sciences, Jinhua, P.R. ChinaInstitute of Quality and Standards for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. ChinaInstitute of Quality and Standards for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. ChinaThe metabolic difference in caecal digesta between Jinhua pig and Landrace pig was compared. Twenty weaned piglets at 28 days of age, including ten Landrace pigs (a Western pig breed) and ten Jinhua pigs (a Chinese native pig breed), were randomly selected and allocated into two groups. The pigs were fed the same corn-soybean diet on the same pig farm. At the age of 240 days, all pigs of each group were slaughtered, the digesta in the caecum of the twenty pigs were collected for metabolomic analysis and determination of short-chain fatty acids (SCFAs). The results showed that a total of 56 different metabolites (22 metabolites named and 34 metabolites without identification) were detected in caecal digesta using a gas chromatography time-of-flight/mass spectrometry (GC-TOF-MS)-based metabolomic approach. Forty-six of the 56 metabolites were upregulated significantly (P < 0.05) in Landrace group compared with Jinhua group. The metabolic pathways with different impact value in which different metabolites were mainly involved were tyrosine metabolism, citrate cycle and steroid biosynthesis. In addition, we found that Landrace accumulated more SCFAs in caecal digesta, while the concentrations of acetic acid (P < 0.01) and butyric acid (P < 0.05) in caecal digesta of Jinhua pig were markedly lower than those of Landrace pig. Collectively, our study was the first to compare the metabolic difference in caecal digesta between Jinhua pig and Landrace pig using a metabolomics approach, which might be used as a potential metabolomics mechanism to research different breeds of pigs.https://cjas.agriculturejournals.cz/artkey/cjs-201908-0002_comparative-metabolomic-analysis-of-caecal-digesta-between-jinhua-pig-and-landrace-pig.phpcaecal digestajinhua piglandrace pigmetabolic differenceshort-chain fatty acids |
| spellingShingle | Yujie Gong Xiaoting Zou Wenrui Xia Xueting Wen Xiaojun Zhang Yingping Xiao Hua Yang Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig Czech Journal of Animal Science caecal digesta jinhua pig landrace pig metabolic difference short-chain fatty acids |
| title | Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig |
| title_full | Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig |
| title_fullStr | Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig |
| title_full_unstemmed | Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig |
| title_short | Comparative metabolomic analysis of caecal digesta between Jinhua pig and Landrace pig |
| title_sort | comparative metabolomic analysis of caecal digesta between jinhua pig and landrace pig |
| topic | caecal digesta jinhua pig landrace pig metabolic difference short-chain fatty acids |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201908-0002_comparative-metabolomic-analysis-of-caecal-digesta-between-jinhua-pig-and-landrace-pig.php |
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