Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen
<p>Abstract</p> <p>Background</p> <p>Cyclamen is a popular and economically significant pot plant crop in several countries. Molecular breeding technologies provide opportunities to metabolically engineer the well-characterized flavonoid biosynthetic pathway for altered...
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| Format: | Article |
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BMC
2010-06-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/107 |
| _version_ | 1811265923424714752 |
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| author | Patel Deepa Marshall Gayle B Davies Kevin M Lewis David H Boase Murray R Schwinn Kathy E Deroles Simon C |
| author_facet | Patel Deepa Marshall Gayle B Davies Kevin M Lewis David H Boase Murray R Schwinn Kathy E Deroles Simon C |
| author_sort | Patel Deepa |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cyclamen is a popular and economically significant pot plant crop in several countries. Molecular breeding technologies provide opportunities to metabolically engineer the well-characterized flavonoid biosynthetic pathway for altered anthocyanin profile and hence the colour of the flower. Previously we reported on a genetic transformation system for cyclamen. Our aim in this study was to change pigment profiles and flower colours in cyclamen through the suppression of flavonoid 3', 5'-hydroxylase, an enzyme in the flavonoid pathway that plays a determining role in the colour of anthocyanin pigments.</p> <p>Results</p> <p>A full-length cDNA putatively identified as a <it>F3'5'H </it>(<it>CpF3'5'H</it>) was isolated from cyclamen flower tissue. Amino acid and phylogeny analyses indicated the <it>CpF3'5'H </it>encodes a F3'5'H enzyme. Two cultivars of minicyclamen were transformed via <it>Agrobacterium tumefaciens </it>with an antisense <it>CpF3'5'H </it>construct. Flowers of the transgenic lines showed modified colour and this correlated positively with the loss of endogenous <it>F3'5'H </it>transcript. Changes in observed colour were confirmed by colorimeter measurements, with an overall loss in intensity of colour (C) in the transgenic lines and a shift in hue from purple to red/pink in one cultivar. HPLC analysis showed that delphinidin-derived pigment levels were reduced in transgenic lines relative to control lines while the percentage of cyanidin-derived pigments increased. Total anthocyanin concentration was reduced up to 80% in some transgenic lines and a smaller increase in flavonol concentration was recorded. Differences were also seen in the ratio of flavonol types that accumulated.</p> <p>Conclusion</p> <p>To our knowledge this is the first report of genetic modification of the anthocyanin pathway in the commercially important species cyclamen. The effects of suppressing a key enzyme, F3'5'H, were wide ranging, extending from anthocyanins to other branches of the flavonoid pathway. The results illustrate the complexity involved in modifying a biosynthetic pathway with multiple branch points to different end products and provides important information for future flower colour modification experiments in cyclamen.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
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| spelling | doaj.art-6a1dcaad77784b2b8ef58b469e2d92972022-12-22T03:17:40ZengBMCBMC Plant Biology1471-22292010-06-0110110710.1186/1471-2229-10-107Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamenPatel DeepaMarshall Gayle BDavies Kevin MLewis David HBoase Murray RSchwinn Kathy EDeroles Simon C<p>Abstract</p> <p>Background</p> <p>Cyclamen is a popular and economically significant pot plant crop in several countries. Molecular breeding technologies provide opportunities to metabolically engineer the well-characterized flavonoid biosynthetic pathway for altered anthocyanin profile and hence the colour of the flower. Previously we reported on a genetic transformation system for cyclamen. Our aim in this study was to change pigment profiles and flower colours in cyclamen through the suppression of flavonoid 3', 5'-hydroxylase, an enzyme in the flavonoid pathway that plays a determining role in the colour of anthocyanin pigments.</p> <p>Results</p> <p>A full-length cDNA putatively identified as a <it>F3'5'H </it>(<it>CpF3'5'H</it>) was isolated from cyclamen flower tissue. Amino acid and phylogeny analyses indicated the <it>CpF3'5'H </it>encodes a F3'5'H enzyme. Two cultivars of minicyclamen were transformed via <it>Agrobacterium tumefaciens </it>with an antisense <it>CpF3'5'H </it>construct. Flowers of the transgenic lines showed modified colour and this correlated positively with the loss of endogenous <it>F3'5'H </it>transcript. Changes in observed colour were confirmed by colorimeter measurements, with an overall loss in intensity of colour (C) in the transgenic lines and a shift in hue from purple to red/pink in one cultivar. HPLC analysis showed that delphinidin-derived pigment levels were reduced in transgenic lines relative to control lines while the percentage of cyanidin-derived pigments increased. Total anthocyanin concentration was reduced up to 80% in some transgenic lines and a smaller increase in flavonol concentration was recorded. Differences were also seen in the ratio of flavonol types that accumulated.</p> <p>Conclusion</p> <p>To our knowledge this is the first report of genetic modification of the anthocyanin pathway in the commercially important species cyclamen. The effects of suppressing a key enzyme, F3'5'H, were wide ranging, extending from anthocyanins to other branches of the flavonoid pathway. The results illustrate the complexity involved in modifying a biosynthetic pathway with multiple branch points to different end products and provides important information for future flower colour modification experiments in cyclamen.</p>http://www.biomedcentral.com/1471-2229/10/107 |
| spellingShingle | Patel Deepa Marshall Gayle B Davies Kevin M Lewis David H Boase Murray R Schwinn Kathy E Deroles Simon C Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen BMC Plant Biology |
| title | Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen |
| title_full | Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen |
| title_fullStr | Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen |
| title_full_unstemmed | Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen |
| title_short | Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen |
| title_sort | isolation and antisense suppression of it flavonoid 3 5 hydroxylase it modifies flower pigments and colour in cyclamen |
| url | http://www.biomedcentral.com/1471-2229/10/107 |
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