Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions
<i>Campylobacter jejuni</i> is the leading cause of bacterial foodborne gastroenteritis worldwide but is rarely transferred between human hosts. Although a recognized microaerophile, the majority of <i>C. jejuni</i> are incapable of growing in an aerobic environment. The pers...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/1894 |
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| author | Fauzy Nasher Burhan Lehri Megan F. Horney Richard A. Stabler Brendan W. Wren |
| author_facet | Fauzy Nasher Burhan Lehri Megan F. Horney Richard A. Stabler Brendan W. Wren |
| author_sort | Fauzy Nasher |
| collection | DOAJ |
| description | <i>Campylobacter jejuni</i> is the leading cause of bacterial foodborne gastroenteritis worldwide but is rarely transferred between human hosts. Although a recognized microaerophile, the majority of <i>C. jejuni</i> are incapable of growing in an aerobic environment. The persistence and transmission of this pathogen outside its warm-blooded avian and mammalian hosts is poorly understood. <i>Acanthamoebae</i> species are predatory protists and form an important ecological niche with several bacterial species. Here, we investigate the interaction of <i>C. jejuni</i> 11168H and <i>Acanthamoebae castellanii</i> at the single-cell level. We observe that a subpopulation of <i>C. jejuni</i> cells can resist killing by <i>A. castellanii,</i> and non-digested bacteria are exocytosed into the environment where they can persist. In addition, we observe that <i>A. castellanii</i> can harbor <i>C. jejuni</i> 11168H even upon encystment. Transcriptome analyses of <i>C. jejuni</i> interactions revealed similar survival mechanisms when infecting both <i>A. castellanii</i> and warm-blooded hosts. In particular, nitrosative stress defense mechanisms and flagellum function are important as confirmed by mutational analyses of <i>C. jejuni</i> 11168H. This study describes a new host–pathogen interaction for <i>C. jejuni</i> and confirms that amoebae are transient hosts for the persistence, adaptability, and potential transmission of <i>C. jejuni</i>. |
| first_indexed | 2024-03-09T19:45:23Z |
| format | Article |
| id | doaj.art-6a1fad2149274d91949e423ec19ee825 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:45:23Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6a1fad2149274d91949e423ec19ee8252023-11-24T01:24:59ZengMDPI AGMicroorganisms2076-26072022-09-011010189410.3390/microorganisms10101894Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen InteractionsFauzy Nasher0Burhan Lehri1Megan F. Horney2Richard A. Stabler3Brendan W. Wren4Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UKFaculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UKFaculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UKFaculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UKFaculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK<i>Campylobacter jejuni</i> is the leading cause of bacterial foodborne gastroenteritis worldwide but is rarely transferred between human hosts. Although a recognized microaerophile, the majority of <i>C. jejuni</i> are incapable of growing in an aerobic environment. The persistence and transmission of this pathogen outside its warm-blooded avian and mammalian hosts is poorly understood. <i>Acanthamoebae</i> species are predatory protists and form an important ecological niche with several bacterial species. Here, we investigate the interaction of <i>C. jejuni</i> 11168H and <i>Acanthamoebae castellanii</i> at the single-cell level. We observe that a subpopulation of <i>C. jejuni</i> cells can resist killing by <i>A. castellanii,</i> and non-digested bacteria are exocytosed into the environment where they can persist. In addition, we observe that <i>A. castellanii</i> can harbor <i>C. jejuni</i> 11168H even upon encystment. Transcriptome analyses of <i>C. jejuni</i> interactions revealed similar survival mechanisms when infecting both <i>A. castellanii</i> and warm-blooded hosts. In particular, nitrosative stress defense mechanisms and flagellum function are important as confirmed by mutational analyses of <i>C. jejuni</i> 11168H. This study describes a new host–pathogen interaction for <i>C. jejuni</i> and confirms that amoebae are transient hosts for the persistence, adaptability, and potential transmission of <i>C. jejuni</i>.https://www.mdpi.com/2076-2607/10/10/1894<i>Campylobacter jejuni</i><i>Acanthamoebae castellanii</i>intra-amoebaesingle-cellhost–pathogen interaction |
| spellingShingle | Fauzy Nasher Burhan Lehri Megan F. Horney Richard A. Stabler Brendan W. Wren Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions Microorganisms <i>Campylobacter jejuni</i> <i>Acanthamoebae castellanii</i> intra-amoebae single-cell host–pathogen interaction |
| title | Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions |
| title_full | Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions |
| title_fullStr | Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions |
| title_full_unstemmed | Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions |
| title_short | Survival of <i>Campylobacter jejuni</i> 11168H in <i>Acanthamoebae castellanii</i> Provides Mechanistic Insight into Host Pathogen Interactions |
| title_sort | survival of i campylobacter jejuni i 11168h in i acanthamoebae castellanii i provides mechanistic insight into host pathogen interactions |
| topic | <i>Campylobacter jejuni</i> <i>Acanthamoebae castellanii</i> intra-amoebae single-cell host–pathogen interaction |
| url | https://www.mdpi.com/2076-2607/10/10/1894 |
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