Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins
The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate via time-lapse imaging distinct phenotypes of prostate organoids derived from pa...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1083150/full |
| _version_ | 1797951931640971264 |
|---|---|
| author | Kendra D. Marr Kendra D. Marr Natalia A. Ignatenko Noel A. Warfel Noel A. Warfel Ken Batai Anne E. Cress Anne E. Cress Grant R. Pollock Ava C. Wong Benjamin R. Lee |
| author_facet | Kendra D. Marr Kendra D. Marr Natalia A. Ignatenko Noel A. Warfel Noel A. Warfel Ken Batai Anne E. Cress Anne E. Cress Grant R. Pollock Ava C. Wong Benjamin R. Lee |
| author_sort | Kendra D. Marr |
| collection | DOAJ |
| description | The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate via time-lapse imaging distinct phenotypes of prostate organoids derived from patient material– without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p<0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology. |
| first_indexed | 2024-04-10T22:39:33Z |
| format | Article |
| id | doaj.art-6a207af412b74d26950181a2ad781c1b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T22:39:33Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-6a207af412b74d26950181a2ad781c1b2023-01-16T08:57:28ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-01-011210.3389/fonc.2022.10831501083150Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteinsKendra D. Marr0Kendra D. Marr1Natalia A. Ignatenko2Noel A. Warfel3Noel A. Warfel4Ken Batai5Anne E. Cress6Anne E. Cress7Grant R. Pollock8Ava C. Wong9Benjamin R. Lee10Cancer Biology, University of Arizona, Tucson, AZ, United StatesMD/PhD Program, College of Medicine Tucson, University of Arizona, Tucson, AZ, United StatesCancer Biology, University of Arizona, Tucson, AZ, United StatesCancer Biology, University of Arizona, Tucson, AZ, United StatesCellular & Molecular Medicine, University of Arizona, Tucson, AZ, United StatesCancer Prevention & Control, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United StatesCancer Biology, University of Arizona, Tucson, AZ, United StatesCellular & Molecular Medicine, University of Arizona, Tucson, AZ, United StatesUrology, College of Medicine Tucson, University of Arizona, Tucson, AZ, United StatesCollege of Nursing, University of Arizona, Tucson, AZ, United StatesUrology, College of Medicine Tucson, University of Arizona, Tucson, AZ, United StatesThe advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate via time-lapse imaging distinct phenotypes of prostate organoids derived from patient material– without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p<0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology.https://www.frontiersin.org/articles/10.3389/fonc.2022.1083150/fullorganoidsprostate cancervideo microscopycancer phenotypesextracellular matrix |
| spellingShingle | Kendra D. Marr Kendra D. Marr Natalia A. Ignatenko Noel A. Warfel Noel A. Warfel Ken Batai Anne E. Cress Anne E. Cress Grant R. Pollock Ava C. Wong Benjamin R. Lee Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins Frontiers in Oncology organoids prostate cancer video microscopy cancer phenotypes extracellular matrix |
| title | Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| title_full | Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| title_fullStr | Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| title_full_unstemmed | Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| title_short | Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| title_sort | digital image analysis using video microscopy of human derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins |
| topic | organoids prostate cancer video microscopy cancer phenotypes extracellular matrix |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1083150/full |
| work_keys_str_mv | AT kendradmarr digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT kendradmarr digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT nataliaaignatenko digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT noelawarfel digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT noelawarfel digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT kenbatai digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT anneecress digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT anneecress digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT grantrpollock digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT avacwong digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins AT benjaminrlee digitalimageanalysisusingvideomicroscopyofhumanderivedprostatecancervsnormalprostateorganoidstoassessmigratorybehavioronextracellularmatrixproteins |