Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients
BackgroundImmune checkpoint inhibitors (ICI) have shown durable and long-term benefits in a subset of head and neck squamous cell carcinoma (HNSCC) patients. To identify patient-responders from non-responders, biomarkers are needed which are predictive of outcome to ICI therapy. Cues in the tumor mi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2020.607349/full |
| _version_ | 1818839055152971776 |
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| author | Arutha Kulasinghe Arutha Kulasinghe Touraj Taheri Touraj Taheri Ken O’Byrne Ken O’Byrne Ken O’Byrne Brett G. M. Hughes Brett G. M. Hughes Liz Kenny Liz Kenny Chamindie Punyadeera Chamindie Punyadeera |
| author_facet | Arutha Kulasinghe Arutha Kulasinghe Touraj Taheri Touraj Taheri Ken O’Byrne Ken O’Byrne Ken O’Byrne Brett G. M. Hughes Brett G. M. Hughes Liz Kenny Liz Kenny Chamindie Punyadeera Chamindie Punyadeera |
| author_sort | Arutha Kulasinghe |
| collection | DOAJ |
| description | BackgroundImmune checkpoint inhibitors (ICI) have shown durable and long-term benefits in a subset of head and neck squamous cell carcinoma (HNSCC) patients. To identify patient-responders from non-responders, biomarkers are needed which are predictive of outcome to ICI therapy. Cues in the tumor microenvironment (TME) have been informative in understanding the tumor-immune contexture.MethodsIn this preliminary study, the NanoString GeoMx™ Digital Spatial Profiling (DSP) technology was used to determine the immune marker and compartment specific measurements in a cohort of HNSCC tumors from patients receiving ICI therapy.ResultsOur data revealed that markers involved with immune cell infiltration (CD8 T-cells) were not predictive of outcome to ICI therapy. Rather, a number of immune cell types and protein markers (CD4, CD68, CD45, CD44, CD66b) were found to correlate with progressive disease. Cross platform comparison with the Opal Vectra (Perkin Elmer) for a number of markers across similar regions of interest demonstrated concordance for pan-cytokeratin, CD8, and PD-L1.ConclusionThis study, to our knowledge, represents the first digital spatial analysis of HNSCC tumors. A larger cohort of HNSCC will be required to orthogonally validate the findings. |
| first_indexed | 2024-12-19T03:48:12Z |
| format | Article |
| id | doaj.art-6a213bef28fb44878855ffa65fbaa711 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-19T03:48:12Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-6a213bef28fb44878855ffa65fbaa7112022-12-21T20:37:03ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-01-011010.3389/fonc.2020.607349607349Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma PatientsArutha Kulasinghe0Arutha Kulasinghe1Touraj Taheri2Touraj Taheri3Ken O’Byrne4Ken O’Byrne5Ken O’Byrne6Brett G. M. Hughes7Brett G. M. Hughes8Liz Kenny9Liz Kenny10Chamindie Punyadeera11Chamindie Punyadeera12The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaDepartment of Pathology, Royal Brisbane and Women’s Hospital, Brisbane, QLD, AustraliaRoyal Brisbane and Women’s Hospital, Herston, QLD, AustraliaThe School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaPrincess Alexandra Hospital, Woolloongabba, QLD, AustraliaRoyal Brisbane and Women’s Hospital, Herston, QLD, AustraliaSchool of Medicine, University of Queensland, Brisbane, QLD, AustraliaRoyal Brisbane and Women’s Hospital, Herston, QLD, AustraliaSchool of Medicine, University of Queensland, Brisbane, QLD, AustraliaThe School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaBackgroundImmune checkpoint inhibitors (ICI) have shown durable and long-term benefits in a subset of head and neck squamous cell carcinoma (HNSCC) patients. To identify patient-responders from non-responders, biomarkers are needed which are predictive of outcome to ICI therapy. Cues in the tumor microenvironment (TME) have been informative in understanding the tumor-immune contexture.MethodsIn this preliminary study, the NanoString GeoMx™ Digital Spatial Profiling (DSP) technology was used to determine the immune marker and compartment specific measurements in a cohort of HNSCC tumors from patients receiving ICI therapy.ResultsOur data revealed that markers involved with immune cell infiltration (CD8 T-cells) were not predictive of outcome to ICI therapy. Rather, a number of immune cell types and protein markers (CD4, CD68, CD45, CD44, CD66b) were found to correlate with progressive disease. Cross platform comparison with the Opal Vectra (Perkin Elmer) for a number of markers across similar regions of interest demonstrated concordance for pan-cytokeratin, CD8, and PD-L1.ConclusionThis study, to our knowledge, represents the first digital spatial analysis of HNSCC tumors. A larger cohort of HNSCC will be required to orthogonally validate the findings.https://www.frontiersin.org/articles/10.3389/fonc.2020.607349/fullhead and neck cancerhead and neck squamous cell carcinomatumor microenvironmentdigital spatial profilingimmunotherapy |
| spellingShingle | Arutha Kulasinghe Arutha Kulasinghe Touraj Taheri Touraj Taheri Ken O’Byrne Ken O’Byrne Ken O’Byrne Brett G. M. Hughes Brett G. M. Hughes Liz Kenny Liz Kenny Chamindie Punyadeera Chamindie Punyadeera Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients Frontiers in Oncology head and neck cancer head and neck squamous cell carcinoma tumor microenvironment digital spatial profiling immunotherapy |
| title | Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients |
| title_full | Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients |
| title_fullStr | Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients |
| title_full_unstemmed | Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients |
| title_short | Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients |
| title_sort | highly multiplexed digital spatial profiling of the tumor microenvironment of head and neck squamous cell carcinoma patients |
| topic | head and neck cancer head and neck squamous cell carcinoma tumor microenvironment digital spatial profiling immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2020.607349/full |
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