High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers
The increasing prevalence of multi-drug resistant (MDR) <i>Escherichia coli</i> in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant <i>E. coli</i> isolate...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/808 |
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| author | Luciana Sampaio Lima Aldo Aparecido Proietti-Junior Yan Corrêa Rodrigues Marcelo Cleyton da Silva Vieira Luana Nepomuceno Gondim Costa Lima Cintya de Oliveira Souza Verônica Dias Gonçalves Marcelo de Oliveira Lima Dália dos Prazeres Rodrigues Karla Valéria Batista Lima |
| author_facet | Luciana Sampaio Lima Aldo Aparecido Proietti-Junior Yan Corrêa Rodrigues Marcelo Cleyton da Silva Vieira Luana Nepomuceno Gondim Costa Lima Cintya de Oliveira Souza Verônica Dias Gonçalves Marcelo de Oliveira Lima Dália dos Prazeres Rodrigues Karla Valéria Batista Lima |
| author_sort | Luciana Sampaio Lima |
| collection | DOAJ |
| description | The increasing prevalence of multi-drug resistant (MDR) <i>Escherichia coli</i> in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant <i>E. coli</i> isolates from <i>Arapirama gigas</i>, a major fish species in the Brazilian Amazon rivers and fish farms, were characterized regarding their antimicrobial susceptibility, virulence, and genetic diversity. A total of forty (40) specimens of <i>A. gigas</i>, including 20 farmed and 20 wild fish, were included. Thirty-four quinolone-resistant <i>E. coli</i> isolates were phenotypically tested by broth microdilution, while resistance and virulence genes were detected by PCR. Molecular epidemiology and genetic relatedness were analyzed by MLST and PFGE typing. The majority of isolates were classified as MDR and detected harboring <i>bla</i><sub>CTX-M</sub>, <i>qnrA</i> and <i>qnrB</i> genes. Enterotoxigenic <i>E. coli</i> pathotype (ETEC) isolates were presented in low prevalence among farmed animals. MLST and PFGE genotyping revealed a wide genetic background, including the detection of internationally spread clones. The obtained data point out <i>A. gigas</i> as a reservoir in Brazilian Amazon aquatic ecosystems and warns of the interference of AMR strains in wildlife and environmental matrices. |
| first_indexed | 2024-03-09T04:22:58Z |
| format | Article |
| id | doaj.art-6a222574353641e8aed197bdf3c900ac |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:22:58Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6a222574353641e8aed197bdf3c900ac2023-12-03T13:45:13ZengMDPI AGMicroorganisms2076-26072022-04-0110480810.3390/microorganisms10040808High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon RiversLuciana Sampaio Lima0Aldo Aparecido Proietti-Junior1Yan Corrêa Rodrigues2Marcelo Cleyton da Silva Vieira3Luana Nepomuceno Gondim Costa Lima4Cintya de Oliveira Souza5Verônica Dias Gonçalves6Marcelo de Oliveira Lima7Dália dos Prazeres Rodrigues8Karla Valéria Batista Lima9Faculty of Pharmaceutical Sciences, Federal University of Amapá (UNIFAP), Macapá 68903-419, AP, BrazilFaculty of Pharmaceutical Sciences, Federal University of Amapá (UNIFAP), Macapá 68903-419, AP, BrazilBacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, BrazilBacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, BrazilBacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, BrazilBacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, BrazilOswaldo Cruz Institute, Oswaldo Cruz Foundation, Pavilhão Rocha Lima, Enterobacteria Laboratory, Rio de Janeiro 21040-900, RJ, BrazilEnvironment Section, Evandro Chagas Institute (SAMAM/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, BrazilOswaldo Cruz Institute, Oswaldo Cruz Foundation, Pavilhão Rocha Lima, Enterobacteria Laboratory, Rio de Janeiro 21040-900, RJ, BrazilOswaldo Cruz Institute, Oswaldo Cruz Foundation, Pavilhão Rocha Lima, Enterobacteria Laboratory, Rio de Janeiro 21040-900, RJ, BrazilThe increasing prevalence of multi-drug resistant (MDR) <i>Escherichia coli</i> in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant <i>E. coli</i> isolates from <i>Arapirama gigas</i>, a major fish species in the Brazilian Amazon rivers and fish farms, were characterized regarding their antimicrobial susceptibility, virulence, and genetic diversity. A total of forty (40) specimens of <i>A. gigas</i>, including 20 farmed and 20 wild fish, were included. Thirty-four quinolone-resistant <i>E. coli</i> isolates were phenotypically tested by broth microdilution, while resistance and virulence genes were detected by PCR. Molecular epidemiology and genetic relatedness were analyzed by MLST and PFGE typing. The majority of isolates were classified as MDR and detected harboring <i>bla</i><sub>CTX-M</sub>, <i>qnrA</i> and <i>qnrB</i> genes. Enterotoxigenic <i>E. coli</i> pathotype (ETEC) isolates were presented in low prevalence among farmed animals. MLST and PFGE genotyping revealed a wide genetic background, including the detection of internationally spread clones. The obtained data point out <i>A. gigas</i> as a reservoir in Brazilian Amazon aquatic ecosystems and warns of the interference of AMR strains in wildlife and environmental matrices.https://www.mdpi.com/2076-2607/10/4/808quinolone-resistanceantimicrobial resistanceESBLsPMQRgenetic diversitymolecular epidemiology |
| spellingShingle | Luciana Sampaio Lima Aldo Aparecido Proietti-Junior Yan Corrêa Rodrigues Marcelo Cleyton da Silva Vieira Luana Nepomuceno Gondim Costa Lima Cintya de Oliveira Souza Verônica Dias Gonçalves Marcelo de Oliveira Lima Dália dos Prazeres Rodrigues Karla Valéria Batista Lima High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers Microorganisms quinolone-resistance antimicrobial resistance ESBLs PMQR genetic diversity molecular epidemiology |
| title | High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers |
| title_full | High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers |
| title_fullStr | High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers |
| title_full_unstemmed | High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers |
| title_short | High Genetic Diversity and Antimicrobial Resistance in <i>Escherichia col</i><i>i</i> Highlight <i>Arapaima gigas</i> (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers |
| title_sort | high genetic diversity and antimicrobial resistance in i escherichia col i i i i highlight i arapaima gigas i pisces arapaimidae as a reservoir of quinolone resistant strains in brazilian amazon rivers |
| topic | quinolone-resistance antimicrobial resistance ESBLs PMQR genetic diversity molecular epidemiology |
| url | https://www.mdpi.com/2076-2607/10/4/808 |
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