<i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity
<i>Viburnum opulus</i> L. fruit is a rich source of phenolic compounds that may be involved in the prevention of metabolic diseases. The purpose of this study was to determine the effects of <i>Viburnum opulus</i> fresh juice (FJ) and juice purified by solid-phase extraction...
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MDPI AG
2020-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/7/2003 |
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| author | Małgorzata Zakłos-Szyda Nina Pietrzyk Marcin Szustak Anna Podsędek |
| author_facet | Małgorzata Zakłos-Szyda Nina Pietrzyk Marcin Szustak Anna Podsędek |
| author_sort | Małgorzata Zakłos-Szyda |
| collection | DOAJ |
| description | <i>Viburnum opulus</i> L. fruit is a rich source of phenolic compounds that may be involved in the prevention of metabolic diseases. The purpose of this study was to determine the effects of <i>Viburnum opulus</i> fresh juice (FJ) and juice purified by solid-phase extraction (PJ) on the adipogenesis process with murine 3T3-L1 preadipocyte cell line and pancreatic lipase activity in triolein emulsion, as well as their phenolic profiles by UPLC/Q-TOF-MS. Decrease of lipids and triacylglycerol accumulation in differentiated 3T3-L1 cells were in concordance with downregulation of the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPβ/α), and sterol regulatory element-binding protein 1c (SREBP-1c). Furthermore, regulation of PPARγ-mediated β-lactamase expression by <i>V. opulus</i> components in reporter gene assay, as well as their binding affinity to ligand-binding domain of PPARγ, were tested. In addition, the levels of enzymes involved in lipid metabolism, like fatty acid synthase (FAS) or acetyl-CoA carboxylase (ACC), were decreased, along with inflammatory cytokines, like tumor necrosis factorα (TNFα), interleukin-6 (Il-6) and leptin. Moreover, FJ and PJ were able to inhibit pancreatic lipase, which potentially could reduce the fat absorption from the intestinal lumen and the storage of body fat in the adipose tissues. Thirty-two phenolic compounds with chlorogenic acid as the dominant compound were identified in PJ which revealed significant biological activity. These data contribute to elucidate <i>V. opulus</i> juice phenolic compounds’ molecular mechanism in adipogenesis regulation in 3T3-L1 cells and dietary fat lipolysis. |
| first_indexed | 2024-03-10T18:39:31Z |
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| id | doaj.art-6a24605e510b4de58a6c86a7c3649fba |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T18:39:31Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-6a24605e510b4de58a6c86a7c3649fba2023-11-20T05:56:12ZengMDPI AGNutrients2072-66432020-07-01127200310.3390/nu12072003<i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase ActivityMałgorzata Zakłos-Szyda0Nina Pietrzyk1Marcin Szustak2Anna Podsędek3Department of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-924 Łódź, PolandDepartment of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-924 Łódź, PolandDepartment of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-924 Łódź, PolandDepartment of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-924 Łódź, Poland<i>Viburnum opulus</i> L. fruit is a rich source of phenolic compounds that may be involved in the prevention of metabolic diseases. The purpose of this study was to determine the effects of <i>Viburnum opulus</i> fresh juice (FJ) and juice purified by solid-phase extraction (PJ) on the adipogenesis process with murine 3T3-L1 preadipocyte cell line and pancreatic lipase activity in triolein emulsion, as well as their phenolic profiles by UPLC/Q-TOF-MS. Decrease of lipids and triacylglycerol accumulation in differentiated 3T3-L1 cells were in concordance with downregulation of the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPβ/α), and sterol regulatory element-binding protein 1c (SREBP-1c). Furthermore, regulation of PPARγ-mediated β-lactamase expression by <i>V. opulus</i> components in reporter gene assay, as well as their binding affinity to ligand-binding domain of PPARγ, were tested. In addition, the levels of enzymes involved in lipid metabolism, like fatty acid synthase (FAS) or acetyl-CoA carboxylase (ACC), were decreased, along with inflammatory cytokines, like tumor necrosis factorα (TNFα), interleukin-6 (Il-6) and leptin. Moreover, FJ and PJ were able to inhibit pancreatic lipase, which potentially could reduce the fat absorption from the intestinal lumen and the storage of body fat in the adipose tissues. Thirty-two phenolic compounds with chlorogenic acid as the dominant compound were identified in PJ which revealed significant biological activity. These data contribute to elucidate <i>V. opulus</i> juice phenolic compounds’ molecular mechanism in adipogenesis regulation in 3T3-L1 cells and dietary fat lipolysis.https://www.mdpi.com/2072-6643/12/7/2003<i>Viburnum opulus</i>phenolic compoundsadipogenesisPPARγlipase inhibition |
| spellingShingle | Małgorzata Zakłos-Szyda Nina Pietrzyk Marcin Szustak Anna Podsędek <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity Nutrients <i>Viburnum opulus</i> phenolic compounds adipogenesis PPARγ lipase inhibition |
| title | <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity |
| title_full | <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity |
| title_fullStr | <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity |
| title_full_unstemmed | <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity |
| title_short | <i>Viburnum opulus</i> L. Juice Phenolics Inhibit Mouse 3T3-L1 Cells Adipogenesis and Pancreatic Lipase Activity |
| title_sort | i viburnum opulus i l juice phenolics inhibit mouse 3t3 l1 cells adipogenesis and pancreatic lipase activity |
| topic | <i>Viburnum opulus</i> phenolic compounds adipogenesis PPARγ lipase inhibition |
| url | https://www.mdpi.com/2072-6643/12/7/2003 |
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