Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency
It is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocyt...
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| Format: | Article |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Kopylchuk_1_20.pdf |
| _version_ | 1797641754159087616 |
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| author | H. P. Kopylchuk I. M. Nykolaichuk I. S. Lylyk |
| author_facet | H. P. Kopylchuk I. M. Nykolaichuk I. S. Lylyk |
| author_sort | H. P. Kopylchuk |
| collection | DOAJ |
| description | It is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocytes, but citrulline liver metabolism at these conditions remains incompletely clear. In this work, the content of citrulline in the rat liver mitochondrial and cytosolic fractions and the activity of citrulline-degrading enzymes – argininosuccinate synthase and argininosuccinate lyase were investigated. It was found that in the mitochondrial fraction a maximal reduction of the citrulline levels occurred after administration of acetaminophen toxic doses regardless of the protein amount in the ration, while in the cytosolic fraction the alimentary protein deficiency was a key factor in decreasing the activity of argininosuccinate synthase and arginino-succinate lyase. The data obtained indicated the disturbances of the urea cycle functioning and explained the decrease of L-arginine level in hepatocytes in conditions of acetaminophen-induced toxic injury against the background alimentary protein deficiency. |
| first_indexed | 2024-03-11T13:50:11Z |
| format | Article |
| id | doaj.art-6a276c3c1ac24eb9ae05d205c75c61a7 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:50:11Z |
| publishDate | 2020-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-6a276c3c1ac24eb9ae05d205c75c61a72023-11-02T09:10:47ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032020-02-0192111311910.15407/ubj92.01.113Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiencyH. P. Kopylchuk0I. M. Nykolaichuk1I. S. Lylyk2Yuriy Fedkovych Chernivtsi National University, Ukraine; Institute of Biology, Chemistry and Bioresources, Chernivtsi, UkraineYuriy Fedkovych Chernivtsi National University, Ukraine; Institute of Biology, Chemistry and Bioresources, Chernivtsi, UkraineYuriy Fedkovych Chernivtsi National University, Ukraine; Institute of Biology, Chemistry and Bioresources, Chernivtsi, UkraineIt is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocytes, but citrulline liver metabolism at these conditions remains incompletely clear. In this work, the content of citrulline in the rat liver mitochondrial and cytosolic fractions and the activity of citrulline-degrading enzymes – argininosuccinate synthase and argininosuccinate lyase were investigated. It was found that in the mitochondrial fraction a maximal reduction of the citrulline levels occurred after administration of acetaminophen toxic doses regardless of the protein amount in the ration, while in the cytosolic fraction the alimentary protein deficiency was a key factor in decreasing the activity of argininosuccinate synthase and arginino-succinate lyase. The data obtained indicated the disturbances of the urea cycle functioning and explained the decrease of L-arginine level in hepatocytes in conditions of acetaminophen-induced toxic injury against the background alimentary protein deficiency.http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Kopylchuk_1_20.pdfacetaminophenalimentary deprivation of proteinargininosuccinate lyasel-сitrullinetoxic injuryаrgininosuccinate synthetase |
| spellingShingle | H. P. Kopylchuk I. M. Nykolaichuk I. S. Lylyk Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency The Ukrainian Biochemical Journal acetaminophen alimentary deprivation of protein argininosuccinate lyase l-сitrulline toxic injury аrgininosuccinate synthetase |
| title | Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| title_full | Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| title_fullStr | Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| title_full_unstemmed | Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| title_short | Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| title_sort | indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency |
| topic | acetaminophen alimentary deprivation of protein argininosuccinate lyase l-сitrulline toxic injury аrgininosuccinate synthetase |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2020/02/Kopylchuk_1_20.pdf |
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