Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus.
West Nile Virus (WNV) is a life threatening flavivirus that causes significant morbidity and mortality worldwide. No preventive therapeutics including vaccines against WNV are available for human use. In this study, immunoinformatics approach was performed to design a multi epitope-based subunit vac...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0253393 |
| _version_ | 1818342761999368192 |
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| author | Md Tahsin Khan Rahatul Islam Tarhima Jahan Jerin Araf Mahmud Sahara Khatun Ahasanul Kobir Md Nahidul Islam Arzuba Akter Shakhinur Islam Mondal |
| author_facet | Md Tahsin Khan Rahatul Islam Tarhima Jahan Jerin Araf Mahmud Sahara Khatun Ahasanul Kobir Md Nahidul Islam Arzuba Akter Shakhinur Islam Mondal |
| author_sort | Md Tahsin Khan |
| collection | DOAJ |
| description | West Nile Virus (WNV) is a life threatening flavivirus that causes significant morbidity and mortality worldwide. No preventive therapeutics including vaccines against WNV are available for human use. In this study, immunoinformatics approach was performed to design a multi epitope-based subunit vaccine against this deadly pathogen. Human (HLA) and Mice (H-2) allele specific potential T-cell and B-cell epitopes were shortlisted through a stringent procedure. Molecular docking showed selected epitopes that have stronger binding affinity with human TLR-4. Molecular dynamics simulation confirmed the stable nature of the docked complex. Furthermore, in silico cloning analysis ensures efficient expression of desired gene in the microbial system. Interestingly, previous studies showed that two of our selected epitopes have strong immune response against WNV. Therefore, selected epitopes could be strong vaccine candidates to prevent WNV infections in human. However, further in vitro and in vivo investigations could be strengthening the validation of the vaccine candidate against WNV. |
| first_indexed | 2024-12-13T16:19:50Z |
| format | Article |
| id | doaj.art-6a2848742a604fe2bd0a6184aaaa15b0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T16:19:50Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6a2848742a604fe2bd0a6184aaaa15b02022-12-21T23:38:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01166e025339310.1371/journal.pone.0253393Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus.Md Tahsin KhanRahatul IslamTarhima Jahan JerinAraf MahmudSahara KhatunAhasanul KobirMd Nahidul IslamArzuba AkterShakhinur Islam MondalWest Nile Virus (WNV) is a life threatening flavivirus that causes significant morbidity and mortality worldwide. No preventive therapeutics including vaccines against WNV are available for human use. In this study, immunoinformatics approach was performed to design a multi epitope-based subunit vaccine against this deadly pathogen. Human (HLA) and Mice (H-2) allele specific potential T-cell and B-cell epitopes were shortlisted through a stringent procedure. Molecular docking showed selected epitopes that have stronger binding affinity with human TLR-4. Molecular dynamics simulation confirmed the stable nature of the docked complex. Furthermore, in silico cloning analysis ensures efficient expression of desired gene in the microbial system. Interestingly, previous studies showed that two of our selected epitopes have strong immune response against WNV. Therefore, selected epitopes could be strong vaccine candidates to prevent WNV infections in human. However, further in vitro and in vivo investigations could be strengthening the validation of the vaccine candidate against WNV.https://doi.org/10.1371/journal.pone.0253393 |
| spellingShingle | Md Tahsin Khan Rahatul Islam Tarhima Jahan Jerin Araf Mahmud Sahara Khatun Ahasanul Kobir Md Nahidul Islam Arzuba Akter Shakhinur Islam Mondal Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. PLoS ONE |
| title | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. |
| title_full | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. |
| title_fullStr | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. |
| title_full_unstemmed | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. |
| title_short | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus. |
| title_sort | immunoinformatics and molecular dynamics approaches next generation vaccine design against west nile virus |
| url | https://doi.org/10.1371/journal.pone.0253393 |
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