Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation
Long QT syndrome (LQT) is an inherited primary arrhythmic disorder characterized by prolonged QT interval on the surface electrocardiogram and life-threatening arrhythmia. In this study, a skin biopsy was obtained from an LQT type 2 (LQT2) patient, who carried a nonsense mutation (c.1956C > A; p....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122001623 |
| _version_ | 1817968061207019520 |
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| author | Fengfeng Guo Yaxun Sun Hongkun Wang Hao Wang Jingjun Zhou Hangping Fan Jun Su Tingyu Gong Chenyang Jiang Ping Liang |
| author_facet | Fengfeng Guo Yaxun Sun Hongkun Wang Hao Wang Jingjun Zhou Hangping Fan Jun Su Tingyu Gong Chenyang Jiang Ping Liang |
| author_sort | Fengfeng Guo |
| collection | DOAJ |
| description | Long QT syndrome (LQT) is an inherited primary arrhythmic disorder characterized by prolonged QT interval on the surface electrocardiogram and life-threatening arrhythmia. In this study, a skin biopsy was obtained from an LQT type 2 (LQT2) patient, who carried a nonsense mutation (c.1956C > A; p.Y652X) in the potassium voltage-gated channel subfamily H member 2 (KCNH2) gene. The skin fibroblasts were reprogrammed by non-integrated Sendai viral method to generate a patient-specific induced pluripotent stem cell (iPSC) line. The generated iPSC line showed typical embryonic stem cell-like morphology, exhibited normal karyotype, expressed pluripotency markers, and was capable to differentiate into three germ layers. |
| first_indexed | 2024-04-13T20:04:02Z |
| format | Article |
| id | doaj.art-6a2b884e7a2e48bebf40b6996c58cd42 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-13T20:04:02Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-6a2b884e7a2e48bebf40b6996c58cd422022-12-22T02:32:07ZengElsevierStem Cell Research1873-50612022-07-0162102813Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutationFengfeng Guo0Yaxun Sun1Hongkun Wang2Hao Wang3Jingjun Zhou4Hangping Fan5Jun Su6Tingyu Gong7Chenyang Jiang8Ping Liang9Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaDepartment of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaDepartment of Prenatal Diagnosis (Screening) Center, Hangzhou Women's Hospital (Hangzhou Maternity and Child Health Care Hospital), Hangzhou 310008, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, ChinaDepartment of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China; Corresponding authors at: 79 Qingchun Road, Hangzhou 310003, China (Ping Liang); 3 Qingchun East Road, Hangzhou 310016, China (Chenyang Jiang).Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China; Corresponding authors at: 79 Qingchun Road, Hangzhou 310003, China (Ping Liang); 3 Qingchun East Road, Hangzhou 310016, China (Chenyang Jiang).Long QT syndrome (LQT) is an inherited primary arrhythmic disorder characterized by prolonged QT interval on the surface electrocardiogram and life-threatening arrhythmia. In this study, a skin biopsy was obtained from an LQT type 2 (LQT2) patient, who carried a nonsense mutation (c.1956C > A; p.Y652X) in the potassium voltage-gated channel subfamily H member 2 (KCNH2) gene. The skin fibroblasts were reprogrammed by non-integrated Sendai viral method to generate a patient-specific induced pluripotent stem cell (iPSC) line. The generated iPSC line showed typical embryonic stem cell-like morphology, exhibited normal karyotype, expressed pluripotency markers, and was capable to differentiate into three germ layers.http://www.sciencedirect.com/science/article/pii/S1873506122001623 |
| spellingShingle | Fengfeng Guo Yaxun Sun Hongkun Wang Hao Wang Jingjun Zhou Hangping Fan Jun Su Tingyu Gong Chenyang Jiang Ping Liang Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation Stem Cell Research |
| title | Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation |
| title_full | Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation |
| title_fullStr | Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation |
| title_full_unstemmed | Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation |
| title_short | Generation of an induced pluripotent stem cell line from a long QT syndrome patient carrying KCNH2/1956C > A mutation |
| title_sort | generation of an induced pluripotent stem cell line from a long qt syndrome patient carrying kcnh2 1956c a mutation |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122001623 |
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