Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA
The action of UVA radiation (both that derived from solar radiation and that used in the treatment of skin diseases) modifies the function and composition of keratinocyte membranes. Therefore, this study aimed to assess the effects of phytocannabinoids (CBD and CBG), used singly and in combination,...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12424 |
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| author | Adam Wroński Izabela Dobrzyńska Szymon Sękowski Wojciech Łuczaj Ewa Olchowik-Grabarek Elżbieta Skrzydlewska |
| author_facet | Adam Wroński Izabela Dobrzyńska Szymon Sękowski Wojciech Łuczaj Ewa Olchowik-Grabarek Elżbieta Skrzydlewska |
| author_sort | Adam Wroński |
| collection | DOAJ |
| description | The action of UVA radiation (both that derived from solar radiation and that used in the treatment of skin diseases) modifies the function and composition of keratinocyte membranes. Therefore, this study aimed to assess the effects of phytocannabinoids (CBD and CBG), used singly and in combination, on the contents of phospholipids, ceramides, lipid rafts and sialic acid in keratinocyte membranes exposed to UVA radiation, together with their structure and functionality. The phytocannabinoids, especially in combination (CBD+CBG), partially prevented increased levels of phosphatidylinositols and sialic acid from occurring and sphingomyelinase activity after the UVA exposure of keratinocytes. This was accompanied by a reduction in the formation of lipid rafts and malondialdehyde, which correlated with the parameters responsible for the integrity and functionality of the keratinocyte membrane (membrane fluidity and permeability and the activity of transmembrane transporters), compared to UVA-irradiated cells. This suggests that the simultaneous use of two phytocannabinoids may have a protective effect on healthy cells, without significantly reducing the therapeutic effect of UV radiation used to treat skin diseases such as psoriasis. |
| first_indexed | 2024-03-11T00:24:26Z |
| format | Article |
| id | doaj.art-6a2edf3aa83a4ce8a14baaed75ce7726 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:24:26Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6a2edf3aa83a4ce8a14baaed75ce77262023-11-18T23:04:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124151242410.3390/ijms241512424Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVAAdam Wroński0Izabela Dobrzyńska1Szymon Sękowski2Wojciech Łuczaj3Ewa Olchowik-Grabarek4Elżbieta Skrzydlewska5Dermatological Specialized Center “DERMAL” NZOZ in Białystok, Nowy Swiat 17/5, 15-453 Białystok, PolandLaboratory of Bioanalysis, Faculty of Chemistry, University in Białystok, Ciołkowskiego 1K, 15-245 Białystok, PolandLaboratory of Molecular Biophysics, Department of Microbiology and Biotechnology, Faculty of Biology, University in Białystok, Ciołkowskiego 1J, 15-245 Białystok, PolandDepartment of Analytical Chemistry, Medical University of Białystok, Mickiewicza 2D, 15-222 Białystok, PolandLaboratory of Molecular Biophysics, Department of Microbiology and Biotechnology, Faculty of Biology, University in Białystok, Ciołkowskiego 1J, 15-245 Białystok, PolandDepartment of Analytical Chemistry, Medical University of Białystok, Mickiewicza 2D, 15-222 Białystok, PolandThe action of UVA radiation (both that derived from solar radiation and that used in the treatment of skin diseases) modifies the function and composition of keratinocyte membranes. Therefore, this study aimed to assess the effects of phytocannabinoids (CBD and CBG), used singly and in combination, on the contents of phospholipids, ceramides, lipid rafts and sialic acid in keratinocyte membranes exposed to UVA radiation, together with their structure and functionality. The phytocannabinoids, especially in combination (CBD+CBG), partially prevented increased levels of phosphatidylinositols and sialic acid from occurring and sphingomyelinase activity after the UVA exposure of keratinocytes. This was accompanied by a reduction in the formation of lipid rafts and malondialdehyde, which correlated with the parameters responsible for the integrity and functionality of the keratinocyte membrane (membrane fluidity and permeability and the activity of transmembrane transporters), compared to UVA-irradiated cells. This suggests that the simultaneous use of two phytocannabinoids may have a protective effect on healthy cells, without significantly reducing the therapeutic effect of UV radiation used to treat skin diseases such as psoriasis.https://www.mdpi.com/1422-0067/24/15/12424phospholipidsceramideslipid raftsmembrane fluiditymembrane electrical chargetransmembrane transporters |
| spellingShingle | Adam Wroński Izabela Dobrzyńska Szymon Sękowski Wojciech Łuczaj Ewa Olchowik-Grabarek Elżbieta Skrzydlewska Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA International Journal of Molecular Sciences phospholipids ceramides lipid rafts membrane fluidity membrane electrical charge transmembrane transporters |
| title | Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA |
| title_full | Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA |
| title_fullStr | Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA |
| title_full_unstemmed | Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA |
| title_short | Cannabidiol and Cannabigerol Modify the Composition and Physicochemical Properties of Keratinocyte Membranes Exposed to UVA |
| title_sort | cannabidiol and cannabigerol modify the composition and physicochemical properties of keratinocyte membranes exposed to uva |
| topic | phospholipids ceramides lipid rafts membrane fluidity membrane electrical charge transmembrane transporters |
| url | https://www.mdpi.com/1422-0067/24/15/12424 |
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