In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i>
Periodontal disease is a relevant oral disease in dogs and nisin–biogel has been previously proposed to be used in its control. <i>Enterococci</i>, as inhabitants of the oral cavity with a high genetic versatility, are a reliable bacterial model for antimicrobial studies. Our goal was to...
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MDPI AG
2023-02-01
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author | Eva Cunha Ana Filipa Ferreira Sara Valente Alice Matos Luís Miguel Carreira Marta Videira Lélia Chambel Luís Tavares Manuela Oliveira |
author_facet | Eva Cunha Ana Filipa Ferreira Sara Valente Alice Matos Luís Miguel Carreira Marta Videira Lélia Chambel Luís Tavares Manuela Oliveira |
author_sort | Eva Cunha |
collection | DOAJ |
description | Periodontal disease is a relevant oral disease in dogs and nisin–biogel has been previously proposed to be used in its control. <i>Enterococci</i>, as inhabitants of the oral cavity with a high genetic versatility, are a reliable bacterial model for antimicrobial studies. Our goal was to evaluate the in vivo influence of the long-term dental application of the nisin–biogel on the virulence and antimicrobial signatures of canine oral <i>enterococci</i>. Twenty dogs were randomly allocated to one of two groups (treatment group—TG with nisin–biogel dental application, or control group—CG without treatment) and submitted to dental plaque sampling at day 0 and after 90 days (T90). Samples were processed for <i>Enterococcus</i> spp. isolation, quantification, identification, molecular typing and antimicrobial and virulence characterization. From a total of 140 <i>enterococci</i>, molecular typing allowed us to obtain 70 representative isolates, mostly identified as <i>E. faecalis</i> and <i>E. faecium</i>. No significant differences (<i>p</i> > 0.05) were observed in the virulence index of the isolates obtained from samples collected from the TG and CG at T90. At T90, a statistically significant difference (<i>p</i> = 0.0008) was observed in the antimicrobial resistance index between the isolates from the TC and CG. Oral <i>enterococci</i> were revealed to be reservoirs of high resistant and virulent phenotypes. |
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issn | 2079-6382 |
language | English |
last_indexed | 2024-03-11T07:01:28Z |
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series | Antibiotics |
spelling | doaj.art-6a2eff7c4ef84d0bbe19f9a75b7975492023-11-17T09:13:23ZengMDPI AGAntibiotics2079-63822023-02-0112346810.3390/antibiotics12030468In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i>Eva Cunha0Ana Filipa Ferreira1Sara Valente2Alice Matos3Luís Miguel Carreira4Marta Videira5Lélia Chambel6Luís Tavares7Manuela Oliveira8CIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCasa dos Animais de Lisboa, Estrada da Pimenteira,1300-459 Lisboa, PortugalBioISI—Biosystems and Integrative Sciences Institute, Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalPeriodontal disease is a relevant oral disease in dogs and nisin–biogel has been previously proposed to be used in its control. <i>Enterococci</i>, as inhabitants of the oral cavity with a high genetic versatility, are a reliable bacterial model for antimicrobial studies. Our goal was to evaluate the in vivo influence of the long-term dental application of the nisin–biogel on the virulence and antimicrobial signatures of canine oral <i>enterococci</i>. Twenty dogs were randomly allocated to one of two groups (treatment group—TG with nisin–biogel dental application, or control group—CG without treatment) and submitted to dental plaque sampling at day 0 and after 90 days (T90). Samples were processed for <i>Enterococcus</i> spp. isolation, quantification, identification, molecular typing and antimicrobial and virulence characterization. From a total of 140 <i>enterococci</i>, molecular typing allowed us to obtain 70 representative isolates, mostly identified as <i>E. faecalis</i> and <i>E. faecium</i>. No significant differences (<i>p</i> > 0.05) were observed in the virulence index of the isolates obtained from samples collected from the TG and CG at T90. At T90, a statistically significant difference (<i>p</i> = 0.0008) was observed in the antimicrobial resistance index between the isolates from the TC and CG. Oral <i>enterococci</i> were revealed to be reservoirs of high resistant and virulent phenotypes.https://www.mdpi.com/2079-6382/12/3/468periodontal diseasedogsnisin–biogel<i>enterococci</i>antimicrobial resistancevirulence signatures |
spellingShingle | Eva Cunha Ana Filipa Ferreira Sara Valente Alice Matos Luís Miguel Carreira Marta Videira Lélia Chambel Luís Tavares Manuela Oliveira In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> Antibiotics periodontal disease dogs nisin–biogel <i>enterococci</i> antimicrobial resistance virulence signatures |
title | In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> |
title_full | In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> |
title_fullStr | In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> |
title_full_unstemmed | In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> |
title_short | In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures of Canine Oral <i>Enterococci</i> |
title_sort | in vivo effect of a nisin biogel on the antimicrobial and virulence signatures of canine oral i enterococci i |
topic | periodontal disease dogs nisin–biogel <i>enterococci</i> antimicrobial resistance virulence signatures |
url | https://www.mdpi.com/2079-6382/12/3/468 |
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