Visualization of PS/γ-Secretase Activity in Living Cells
Summary: A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220303242 |
| _version_ | 1818524043910840320 |
|---|---|
| author | Masato Maesako Nicole M. Sekula Anna Aristarkhova Polina Feschenko Lauren C. Anderson Oksana Berezovska |
| author_facet | Masato Maesako Nicole M. Sekula Anna Aristarkhova Polina Feschenko Lauren C. Anderson Oksana Berezovska |
| author_sort | Masato Maesako |
| collection | DOAJ |
| description | Summary: A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/γ-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitative monitoring of endogenous PS/γ-secretase activity in live cells longitudinally on a cell-by-cell basis. Using these FRET biosensors, we uncovered that PS/γ-secretase activity is heterogeneously regulated among live neurons. |
| first_indexed | 2024-12-11T05:52:18Z |
| format | Article |
| id | doaj.art-6a2fba5afd814e7ab312c1cf43d7c2b6 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-11T05:52:18Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6a2fba5afd814e7ab312c1cf43d7c2b62022-12-22T01:18:47ZengElsevieriScience2589-00422020-06-01236101139Visualization of PS/γ-Secretase Activity in Living CellsMasato Maesako0Nicole M. Sekula1Anna Aristarkhova2Polina Feschenko3Lauren C. Anderson4Oksana Berezovska5Alzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USA; Corresponding authorAlzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USAAlzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USAAlzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USAAlzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USAAlzheimer's Disease Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, 114, 16th Street, Charlestown, MA 02129, USA; Corresponding authorSummary: A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/γ-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitative monitoring of endogenous PS/γ-secretase activity in live cells longitudinally on a cell-by-cell basis. Using these FRET biosensors, we uncovered that PS/γ-secretase activity is heterogeneously regulated among live neurons.http://www.sciencedirect.com/science/article/pii/S2589004220303242Biological SciencesCell BiologyMethodology in Biological SciencesNeuroscience |
| spellingShingle | Masato Maesako Nicole M. Sekula Anna Aristarkhova Polina Feschenko Lauren C. Anderson Oksana Berezovska Visualization of PS/γ-Secretase Activity in Living Cells iScience Biological Sciences Cell Biology Methodology in Biological Sciences Neuroscience |
| title | Visualization of PS/γ-Secretase Activity in Living Cells |
| title_full | Visualization of PS/γ-Secretase Activity in Living Cells |
| title_fullStr | Visualization of PS/γ-Secretase Activity in Living Cells |
| title_full_unstemmed | Visualization of PS/γ-Secretase Activity in Living Cells |
| title_short | Visualization of PS/γ-Secretase Activity in Living Cells |
| title_sort | visualization of ps γ secretase activity in living cells |
| topic | Biological Sciences Cell Biology Methodology in Biological Sciences Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220303242 |
| work_keys_str_mv | AT masatomaesako visualizationofpsgsecretaseactivityinlivingcells AT nicolemsekula visualizationofpsgsecretaseactivityinlivingcells AT annaaristarkhova visualizationofpsgsecretaseactivityinlivingcells AT polinafeschenko visualizationofpsgsecretaseactivityinlivingcells AT laurencanderson visualizationofpsgsecretaseactivityinlivingcells AT oksanaberezovska visualizationofpsgsecretaseactivityinlivingcells |