Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages
The aim of this study was to investigate protein profiles relatedto the induction of adipogenesis within the bovine longissimusdorsi muscle (BLDM) by proteomic analysis. We analyzedBLDM proteins at different growth stages to clarify the physiologicalmechanisms of marbled muscle development in 20head...
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| Format: | Article |
| Language: | English |
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Korean Society for Biochemistry and Molecular Biology
2012-02-01
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| Series: | BMB Reports |
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| Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0yJTVEMTIwMjI3MTA1NV8lMjgxMDItMTA3JTI5Qk1CMTEtMTg0LnBkZg== |
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| author | Yong-Cheng Jin Jeng-A Han Cheng-Xiong Xu Sang-Kee Kang Sang-Hun Kim Kang-Suk Seo Du-Hak Yoon Yun-Jaie Choi Hong-Gu Lee |
| author_facet | Yong-Cheng Jin Jeng-A Han Cheng-Xiong Xu Sang-Kee Kang Sang-Hun Kim Kang-Suk Seo Du-Hak Yoon Yun-Jaie Choi Hong-Gu Lee |
| author_sort | Yong-Cheng Jin |
| collection | DOAJ |
| description | The aim of this study was to investigate protein profiles relatedto the induction of adipogenesis within the bovine longissimusdorsi muscle (BLDM) by proteomic analysis. We analyzedBLDM proteins at different growth stages to clarify the physiologicalmechanisms of marbled muscle development in 20head of Korean native cattle (11 month: 10 head, 17 month: 10head). BLDM proteins were analyzed by two-dimensional electrophoresisand image analysis. Villin 2 was specifically identifiedby mass spectrometry and a protein search engine. Villin 2protein expression in BLDM decreased during the fat developmentstage in test steers. In a Western blot cell culture study ofspontaneously immortal bovine muscle fibroblasts, the abundanceof Villin 2 was shown to be down-regulated during differentiationinto muscle. In 3T3-L1 mouse embryonic fibroblasts,Villin 2 was decreased during differentiation intoadipocytes. The results suggest that Villin 2 may be related tothe induction of transdifferentiation and adipogenesis in bovinelongissimus dorsi muscle. [BMB reports 2012; 45(2): 102-107] |
| first_indexed | 2024-12-20T14:49:53Z |
| format | Article |
| id | doaj.art-6a347c0bbbe94d2394d45e77d81e751b |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-12-20T14:49:53Z |
| publishDate | 2012-02-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-6a347c0bbbe94d2394d45e77d81e751b2022-12-21T19:36:58ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2012-02-01452102107http://dx.doi.org/10.5483/BMBRep.2012.45.2.102Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stagesYong-Cheng JinJeng-A HanCheng-Xiong XuSang-Kee KangSang-Hun KimKang-Suk SeoDu-Hak YoonYun-Jaie ChoiHong-Gu LeeThe aim of this study was to investigate protein profiles relatedto the induction of adipogenesis within the bovine longissimusdorsi muscle (BLDM) by proteomic analysis. We analyzedBLDM proteins at different growth stages to clarify the physiologicalmechanisms of marbled muscle development in 20head of Korean native cattle (11 month: 10 head, 17 month: 10head). BLDM proteins were analyzed by two-dimensional electrophoresisand image analysis. Villin 2 was specifically identifiedby mass spectrometry and a protein search engine. Villin 2protein expression in BLDM decreased during the fat developmentstage in test steers. In a Western blot cell culture study ofspontaneously immortal bovine muscle fibroblasts, the abundanceof Villin 2 was shown to be down-regulated during differentiationinto muscle. In 3T3-L1 mouse embryonic fibroblasts,Villin 2 was decreased during differentiation intoadipocytes. The results suggest that Villin 2 may be related tothe induction of transdifferentiation and adipogenesis in bovinelongissimus dorsi muscle. [BMB reports 2012; 45(2): 102-107]http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0yJTVEMTIwMjI3MTA1NV8lMjgxMDItMTA3JTI5Qk1CMTEtMTg0LnBkZg==Korean native steerMarblingProteomicsVillin 2 |
| spellingShingle | Yong-Cheng Jin Jeng-A Han Cheng-Xiong Xu Sang-Kee Kang Sang-Hun Kim Kang-Suk Seo Du-Hak Yoon Yun-Jaie Choi Hong-Gu Lee Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages BMB Reports Korean native steer Marbling Proteomics Villin 2 |
| title | Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages |
| title_full | Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages |
| title_fullStr | Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages |
| title_full_unstemmed | Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages |
| title_short | Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages |
| title_sort | functional study of villin 2 protein expressed in longissimus dorsi muscle of korean native cattle in different growth stages |
| topic | Korean native steer Marbling Proteomics Villin 2 |
| url | http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0yJTVEMTIwMjI3MTA1NV8lMjgxMDItMTA3JTI5Qk1CMTEtMTg0LnBkZg== |
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