Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i>
AbstractMomilactone B is a natural product with dual biological activities, including antimicrobial and allelopathic properties, and plays a major role in plant chemical defense against competitive plants and pathogens. The pharmacological effects of momilactone B on mammalian cells have also been r...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2021-05-01
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| Series: | G3: Genes, Genomes, Genetics |
| Online Access: | https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkab156 |
| _version_ | 1818323472957308928 |
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| author | Keisuke Tomita Yoko Yashiroda Yasuhiro Matsuo Jeff S Piotrowski Sheena C Li Reika Okamoto Mami Yoshimura Hiromi Kimura Yumi Kawamura Makoto Kawamukai Charles Boone Minoru Yoshida Hideaki Nojiri Kazunori Okada |
| author_facet | Keisuke Tomita Yoko Yashiroda Yasuhiro Matsuo Jeff S Piotrowski Sheena C Li Reika Okamoto Mami Yoshimura Hiromi Kimura Yumi Kawamura Makoto Kawamukai Charles Boone Minoru Yoshida Hideaki Nojiri Kazunori Okada |
| author_sort | Keisuke Tomita |
| collection | DOAJ |
| description | AbstractMomilactone B is a natural product with dual biological activities, including antimicrobial and allelopathic properties, and plays a major role in plant chemical defense against competitive plants and pathogens. The pharmacological effects of momilactone B on mammalian cells have also been reported. However, little is known about the molecular and cellular mechanisms underlying its broad bioactivity. In this study, the genetic determinants of momilactone B sensitivity in yeast were explored to gain insight into its mode of action. We screened fission yeast mutants resistant to momilactone B from a pooled culture containing genome-wide gene-overexpressing strains in a drug-hypersensitive genetic background. Overexpression of pmd1bfr1pap1arp9SPAC9E9.06c |
| first_indexed | 2024-12-13T11:13:15Z |
| format | Article |
| id | doaj.art-6a3499f8f82f45518f828d5b27568453 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-13T11:13:15Z |
| publishDate | 2021-05-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-6a3499f8f82f45518f828d5b275684532022-12-21T23:48:41ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362021-05-0111810.1093/g3journal/jkab156Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i>Keisuke Tomita0https://orcid.org/0000-0002-7324-6613Yoko Yashiroda1Yasuhiro Matsuo2https://orcid.org/0000-0002-2858-7179Jeff S Piotrowski3Sheena C Li4Reika Okamoto5Mami Yoshimura6Hiromi Kimura7Yumi Kawamura8Makoto Kawamukai9Charles Boone10https://orcid.org/0000-0002-3542-6760Minoru Yoshida11Hideaki Nojiri12Kazunori Okada13Agro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanInstitute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue, Shimane 690-8504, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanInstitute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue, Shimane 690-8504, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanRIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, JapanAgro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanAgro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanAbstractMomilactone B is a natural product with dual biological activities, including antimicrobial and allelopathic properties, and plays a major role in plant chemical defense against competitive plants and pathogens. The pharmacological effects of momilactone B on mammalian cells have also been reported. However, little is known about the molecular and cellular mechanisms underlying its broad bioactivity. In this study, the genetic determinants of momilactone B sensitivity in yeast were explored to gain insight into its mode of action. We screened fission yeast mutants resistant to momilactone B from a pooled culture containing genome-wide gene-overexpressing strains in a drug-hypersensitive genetic background. Overexpression of pmd1bfr1pap1arp9SPAC9E9.06chttps://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkab156 |
| spellingShingle | Keisuke Tomita Yoko Yashiroda Yasuhiro Matsuo Jeff S Piotrowski Sheena C Li Reika Okamoto Mami Yoshimura Hiromi Kimura Yumi Kawamura Makoto Kawamukai Charles Boone Minoru Yoshida Hideaki Nojiri Kazunori Okada Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> G3: Genes, Genomes, Genetics |
| title | Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> |
| title_full | Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> |
| title_fullStr | Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> |
| title_full_unstemmed | Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> |
| title_short | Genome-wide screening of genes associated with momilactone B sensitivity in the fission yeast <i>Schizosaccharomyces pombe</i> |
| title_sort | genome wide screening of genes associated with momilactone b sensitivity in the fission yeast i schizosaccharomyces pombe i |
| url | https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkab156 |
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