Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF
Abstract To explore the effect of electroacupuncture on spinal cord injury (SCI) involving immune‐related factors and regeneration‐related factors in rats. The model of spinal cord contusion was established by PCI 3000 instrument. Two types of acupuncture points were selected for electroacupuncture...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-09-01
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| Series: | Ibrain |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ibra.12055 |
| _version_ | 1818063151479914496 |
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| author | Bao‐Lei Zhang Xi‐Liang Guo |
| author_facet | Bao‐Lei Zhang Xi‐Liang Guo |
| author_sort | Bao‐Lei Zhang |
| collection | DOAJ |
| description | Abstract To explore the effect of electroacupuncture on spinal cord injury (SCI) involving immune‐related factors and regeneration‐related factors in rats. The model of spinal cord contusion was established by PCI 3000 instrument. Two types of acupuncture points were selected for electroacupuncture treatment on rats. The rats were tested once a week, and the fiber remodeling was detected by magnetic resonance imaging. Transcriptome sequencing was performed on spinal scar samples. Using Python to write code, statistical analysis and bioinformatics analysis of the correlation between transcriptome sequencing data and fiber reconstruction results are carried out. Lastly, the expression of CD4 and brain‐derived neurotrophic factor (BDNF) in spinal cord scar was verified by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR). Electroacupuncture exhibited a positive effect on the recovery of motor function in rats after SCI. Bioinformatics analysis found a direct interaction between CD4 and BDNF. Transcriptome sequencing and PCR results verified that electroacupuncture significantly reduced the expression of CD4, and increased significantly the expression of BDNF, simultaneously corresponding to nerve regeneration in rats with SCI. Our results showed that electroacupuncture intervention in SCI rats improves neural behavior via inhibiting the expression of CD4 and increasing the expression of BDNF. |
| first_indexed | 2024-12-10T14:15:33Z |
| format | Article |
| id | doaj.art-6a35268375fc41c5b1fa49c2578d7261 |
| institution | Directory Open Access Journal |
| issn | 2769-2795 |
| language | English |
| last_indexed | 2024-12-10T14:15:33Z |
| publishDate | 2022-09-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Ibrain |
| spelling | doaj.art-6a35268375fc41c5b1fa49c2578d72612022-12-22T01:45:20ZengWiley-VCHIbrain2769-27952022-09-018328530110.1002/ibra.12055Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNFBao‐Lei Zhang0Xi‐Liang Guo1Department of Anatomy Jinzhou Medical University Jinzhou Liaoning ChinaDepartment of Experimental Zoology Kunming Medical University Kunming Yunnan ChinaAbstract To explore the effect of electroacupuncture on spinal cord injury (SCI) involving immune‐related factors and regeneration‐related factors in rats. The model of spinal cord contusion was established by PCI 3000 instrument. Two types of acupuncture points were selected for electroacupuncture treatment on rats. The rats were tested once a week, and the fiber remodeling was detected by magnetic resonance imaging. Transcriptome sequencing was performed on spinal scar samples. Using Python to write code, statistical analysis and bioinformatics analysis of the correlation between transcriptome sequencing data and fiber reconstruction results are carried out. Lastly, the expression of CD4 and brain‐derived neurotrophic factor (BDNF) in spinal cord scar was verified by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR). Electroacupuncture exhibited a positive effect on the recovery of motor function in rats after SCI. Bioinformatics analysis found a direct interaction between CD4 and BDNF. Transcriptome sequencing and PCR results verified that electroacupuncture significantly reduced the expression of CD4, and increased significantly the expression of BDNF, simultaneously corresponding to nerve regeneration in rats with SCI. Our results showed that electroacupuncture intervention in SCI rats improves neural behavior via inhibiting the expression of CD4 and increasing the expression of BDNF.https://doi.org/10.1002/ibra.12055brain‐derived neurotrophic factor (BDNF)CD4electroacupuncturenerve regenerationspinal cord injury (SCI) |
| spellingShingle | Bao‐Lei Zhang Xi‐Liang Guo Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF Ibrain brain‐derived neurotrophic factor (BDNF) CD4 electroacupuncture nerve regeneration spinal cord injury (SCI) |
| title | Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF |
| title_full | Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF |
| title_fullStr | Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF |
| title_full_unstemmed | Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF |
| title_short | Electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of CD4 and BDNF |
| title_sort | electroacupuncture promotes nerve regeneration and functional recovery in rats with spinal cord contusion through the coordinate interaction of cd4 and bdnf |
| topic | brain‐derived neurotrophic factor (BDNF) CD4 electroacupuncture nerve regeneration spinal cord injury (SCI) |
| url | https://doi.org/10.1002/ibra.12055 |
| work_keys_str_mv | AT baoleizhang electroacupuncturepromotesnerveregenerationandfunctionalrecoveryinratswithspinalcordcontusionthroughthecoordinateinteractionofcd4andbdnf AT xiliangguo electroacupuncturepromotesnerveregenerationandfunctionalrecoveryinratswithspinalcordcontusionthroughthecoordinateinteractionofcd4andbdnf |