The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping
Background: It has been confirmed that the connective tissue growth factor (CTGF) gene rs9402373 polymorphism is associated with fibrotic and inflammatory diseases. However, studies on the relationship between polymorphisms in CTGF rs9402373 and inflammatory bowel disease (IBD) remain rare. Therefor...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402304210X |
| _version_ | 1797807048437530624 |
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| author | Yiyi Xie Ying Ping Pan Yu Weiwei Liu Xinan Chen Qi Wang Yuhua Chen Xiuzhi Duan Xuchu Wang |
| author_facet | Yiyi Xie Ying Ping Pan Yu Weiwei Liu Xinan Chen Qi Wang Yuhua Chen Xiuzhi Duan Xuchu Wang |
| author_sort | Yiyi Xie |
| collection | DOAJ |
| description | Background: It has been confirmed that the connective tissue growth factor (CTGF) gene rs9402373 polymorphism is associated with fibrotic and inflammatory diseases. However, studies on the relationship between polymorphisms in CTGF rs9402373 and inflammatory bowel disease (IBD) remain rare. Therefore, the aim of this study was to assess the association between the CTGF rs9402373 polymorphism and IBD susceptibility in a Chinese population. Materials and methods: To establish an amplification refractory mutation system (ARMS) PCR technology for genotyping CTGF gene rs9402373 polymorphism, we designed two specific forward primers for the wild and mutant types by placing the allele-specific nucleotide at the penultimate position of the ‘3′ end of the primer. Then, 10 samples were randomly selected and rechecked by DNA sequencing to verify the accuracy of this method. We further used the established method to detect specimens collected from 191 patients with inflammatory bowel disease, including 120 Crohn's disease (CD) and 71 ulcerative colitis (UC), and 110 healthy Han Chinese individuals. Results: We successfully established the ARMS-PCR method for genotyping, and the results of 10 randomly selected samples were completely consistent with DNA sequencing. The rs9402373 G allele frequencies in UC and CD cases were 38.03% and 43.75%, respectively, and in controls, they were 41.82%. No significant difference was found in minor allele frequencies between the UC or CD and control groups (P = 0.473, P = 0.676). Genotype analysis demonstrated that there was no relationship between CTGF rs9402373 polymorphism and the risk of IBD regardless of the inheritance mode (P > 0.05). Conclusions: In this preliminary study, we successfully developed a simple, efficient and cost-effective method for genotyping CTGF rs9402373 polymorphism. The polymorphism may not be related to IBD susceptibility in the Chinese Han population. |
| first_indexed | 2024-03-13T06:16:40Z |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:16:40Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-6a357b34ac87432eb91fb66780ffe13f2023-06-11T04:14:45ZengElsevierHeliyon2405-84402023-06-0196e17003The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotypingYiyi Xie0Ying Ping1Pan Yu2Weiwei Liu3Xinan Chen4Qi Wang5Yuhua Chen6Xiuzhi Duan7Xuchu Wang8The Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaHangzhou Medical College, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, ChinaThe Second Affiliated Hospital of Zhejiang University School of Medicine, China; Corresponding author.Background: It has been confirmed that the connective tissue growth factor (CTGF) gene rs9402373 polymorphism is associated with fibrotic and inflammatory diseases. However, studies on the relationship between polymorphisms in CTGF rs9402373 and inflammatory bowel disease (IBD) remain rare. Therefore, the aim of this study was to assess the association between the CTGF rs9402373 polymorphism and IBD susceptibility in a Chinese population. Materials and methods: To establish an amplification refractory mutation system (ARMS) PCR technology for genotyping CTGF gene rs9402373 polymorphism, we designed two specific forward primers for the wild and mutant types by placing the allele-specific nucleotide at the penultimate position of the ‘3′ end of the primer. Then, 10 samples were randomly selected and rechecked by DNA sequencing to verify the accuracy of this method. We further used the established method to detect specimens collected from 191 patients with inflammatory bowel disease, including 120 Crohn's disease (CD) and 71 ulcerative colitis (UC), and 110 healthy Han Chinese individuals. Results: We successfully established the ARMS-PCR method for genotyping, and the results of 10 randomly selected samples were completely consistent with DNA sequencing. The rs9402373 G allele frequencies in UC and CD cases were 38.03% and 43.75%, respectively, and in controls, they were 41.82%. No significant difference was found in minor allele frequencies between the UC or CD and control groups (P = 0.473, P = 0.676). Genotype analysis demonstrated that there was no relationship between CTGF rs9402373 polymorphism and the risk of IBD regardless of the inheritance mode (P > 0.05). Conclusions: In this preliminary study, we successfully developed a simple, efficient and cost-effective method for genotyping CTGF rs9402373 polymorphism. The polymorphism may not be related to IBD susceptibility in the Chinese Han population.http://www.sciencedirect.com/science/article/pii/S240584402304210XInflammatory bowel diseaseConnective tissue growth factorAmplification refractory mutation systemSingle nucleotide polymorphism |
| spellingShingle | Yiyi Xie Ying Ping Pan Yu Weiwei Liu Xinan Chen Qi Wang Yuhua Chen Xiuzhi Duan Xuchu Wang The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping Heliyon Inflammatory bowel disease Connective tissue growth factor Amplification refractory mutation system Single nucleotide polymorphism |
| title | The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping |
| title_full | The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping |
| title_fullStr | The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping |
| title_full_unstemmed | The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping |
| title_short | The rs9402373 polymorphism of CTGF gene may not be related to inflammatory bowel disease susceptibility in Chinese population based on ARMS-PCR genotyping |
| title_sort | rs9402373 polymorphism of ctgf gene may not be related to inflammatory bowel disease susceptibility in chinese population based on arms pcr genotyping |
| topic | Inflammatory bowel disease Connective tissue growth factor Amplification refractory mutation system Single nucleotide polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S240584402304210X |
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