Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture
The effect of <i>Bacillus subtilis</i> in combination with salicylic (SA) and jasmonic (JA) acids on the activity of amylases, cellulases, proteases, and their inhibitors in potato leaves in connection with the development of resistance to <i>Phytophthora infestans</i> (Mont....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | International Journal of Plant Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2037-0164/14/2/27 |
| _version_ | 1797594208043794432 |
|---|---|
| author | Vyacheslav Tsvetkov Liubov Yarullina Antonina Sorokan Vilena Khabibullina Ildar Mardanshin |
| author_facet | Vyacheslav Tsvetkov Liubov Yarullina Antonina Sorokan Vilena Khabibullina Ildar Mardanshin |
| author_sort | Vyacheslav Tsvetkov |
| collection | DOAJ |
| description | The effect of <i>Bacillus subtilis</i> in combination with salicylic (SA) and jasmonic (JA) acids on the activity of amylases, cellulases, proteases, and their inhibitors in potato leaves in connection with the development of resistance to <i>Phytophthora infestans</i> (Mont.) de Bary in conditions of moisture deficiency have been investigated. Plants grown from microtubers were treated with <i>Bacillus subtilis</i> suspension (10<sup>8</sup> cells/mL) and with a mixture of bacteria with SA (10<sup>−6</sup> M), JA (10<sup>−7</sup> M), and SA + JA and were then infected with <i>P. infestans</i> (10<sup>7</sup> spores/mL) and cultivated under drought. Treatment with <i>B. subtilis</i> bacteria, especially in combination with signaling molecules, contributed to a decrease in the degree of pathogen infestation on plants grown with a lack of moisture. Both salicylate and jasmonate signaling pathways play an important role in the regulation of hydrolase activity and the stimulation of plant resistance. The revealed differences in the degree of hydrolase inhibitors activation under the influence of <i>B. subtilis</i> bacteria and signal molecules suggest different paths to the formation of resistance to <i>P. infestans</i> in potato under drought conditions. |
| first_indexed | 2024-03-11T02:20:23Z |
| format | Article |
| id | doaj.art-6a36c1886b714c06b92acce23607f65f |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-11T02:20:23Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-6a36c1886b714c06b92acce23607f65f2023-11-18T10:53:24ZengMDPI AGInternational Journal of Plant Biology2037-01642023-03-0114232933810.3390/ijpb14020027Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of MoistureVyacheslav Tsvetkov0Liubov Yarullina1Antonina Sorokan2Vilena Khabibullina3Ildar Mardanshin4Department ob Biology, Ufa University of Science and Technology, 450076 Ufa, RussiaInstitute of Biochemistry and Genetics, Ufa Federal Research Centre RAS, 450054 Ufa, RussiaInstitute of Biochemistry and Genetics, Ufa Federal Research Centre RAS, 450054 Ufa, RussiaDepartment ob Biology, Ufa University of Science and Technology, 450076 Ufa, RussiaBashkir Research Institute for Agriculture, Ufa Federal Research Centre RAS, 450054 Ufa, RussiaThe effect of <i>Bacillus subtilis</i> in combination with salicylic (SA) and jasmonic (JA) acids on the activity of amylases, cellulases, proteases, and their inhibitors in potato leaves in connection with the development of resistance to <i>Phytophthora infestans</i> (Mont.) de Bary in conditions of moisture deficiency have been investigated. Plants grown from microtubers were treated with <i>Bacillus subtilis</i> suspension (10<sup>8</sup> cells/mL) and with a mixture of bacteria with SA (10<sup>−6</sup> M), JA (10<sup>−7</sup> M), and SA + JA and were then infected with <i>P. infestans</i> (10<sup>7</sup> spores/mL) and cultivated under drought. Treatment with <i>B. subtilis</i> bacteria, especially in combination with signaling molecules, contributed to a decrease in the degree of pathogen infestation on plants grown with a lack of moisture. Both salicylate and jasmonate signaling pathways play an important role in the regulation of hydrolase activity and the stimulation of plant resistance. The revealed differences in the degree of hydrolase inhibitors activation under the influence of <i>B. subtilis</i> bacteria and signal molecules suggest different paths to the formation of resistance to <i>P. infestans</i> in potato under drought conditions.https://www.mdpi.com/2037-0164/14/2/27<i>Solanum tuberosum</i><i>Phytophthora infestans</i><i>Bacillus subtilis</i>salicylic acidjasmonic acidamylases |
| spellingShingle | Vyacheslav Tsvetkov Liubov Yarullina Antonina Sorokan Vilena Khabibullina Ildar Mardanshin Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture International Journal of Plant Biology <i>Solanum tuberosum</i> <i>Phytophthora infestans</i> <i>Bacillus subtilis</i> salicylic acid jasmonic acid amylases |
| title | Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture |
| title_full | Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture |
| title_fullStr | Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture |
| title_full_unstemmed | Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture |
| title_short | Activity of Hydrolases and Their Inhibitors in Potato Plants Treated with <i>Bacillus subtilis</i>, Salicylic, and Jasmonic Acids and Affected by the Combined Effect of the Late Blight and the Lack of Moisture |
| title_sort | activity of hydrolases and their inhibitors in potato plants treated with i bacillus subtilis i salicylic and jasmonic acids and affected by the combined effect of the late blight and the lack of moisture |
| topic | <i>Solanum tuberosum</i> <i>Phytophthora infestans</i> <i>Bacillus subtilis</i> salicylic acid jasmonic acid amylases |
| url | https://www.mdpi.com/2037-0164/14/2/27 |
| work_keys_str_mv | AT vyacheslavtsvetkov activityofhydrolasesandtheirinhibitorsinpotatoplantstreatedwithibacillussubtilisisalicylicandjasmonicacidsandaffectedbythecombinedeffectofthelateblightandthelackofmoisture AT liubovyarullina activityofhydrolasesandtheirinhibitorsinpotatoplantstreatedwithibacillussubtilisisalicylicandjasmonicacidsandaffectedbythecombinedeffectofthelateblightandthelackofmoisture AT antoninasorokan activityofhydrolasesandtheirinhibitorsinpotatoplantstreatedwithibacillussubtilisisalicylicandjasmonicacidsandaffectedbythecombinedeffectofthelateblightandthelackofmoisture AT vilenakhabibullina activityofhydrolasesandtheirinhibitorsinpotatoplantstreatedwithibacillussubtilisisalicylicandjasmonicacidsandaffectedbythecombinedeffectofthelateblightandthelackofmoisture AT ildarmardanshin activityofhydrolasesandtheirinhibitorsinpotatoplantstreatedwithibacillussubtilisisalicylicandjasmonicacidsandaffectedbythecombinedeffectofthelateblightandthelackofmoisture |