Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008
<p>Abstract</p> <p>This study describes the prevalence of culture-positive adenovirus serotypes in culture-positive respiratory specimens sent to the Central Public Health Laboratory, Toronto, Ontario, Canada for the period September 2007–June 2008. Total nucleic acid was extracted...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-01-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/11 |
| _version_ | 1811284785272717312 |
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| author | Mazzulli Tony Blair Joanne Lombos Ernesto Eshaghi AliReza Yeung Rani Burton Laura Drews Steven J |
| author_facet | Mazzulli Tony Blair Joanne Lombos Ernesto Eshaghi AliReza Yeung Rani Burton Laura Drews Steven J |
| author_sort | Mazzulli Tony |
| collection | DOAJ |
| description | <p>Abstract</p> <p>This study describes the prevalence of culture-positive adenovirus serotypes in culture-positive respiratory specimens sent to the Central Public Health Laboratory, Toronto, Ontario, Canada for the period September 2007–June 2008. Total nucleic acid was extracted from virus cultures using an automated extraction method followed by polymerase chain reaction and Sanger sequencing of the adenovirus hexon gene hypervariable region 7. 73% of specimens (n = 70) were from patients ≤ 4 years of age. Of the 96 adenovirus isolates, the most common identified serotypes were serotype 3 (n = 44, 46%), serotype 2 (n = 25, 26%), serotype 1 (n = 17, 18%), and serotype 21 (n = 5, 5%). Adenovirus serotype 14 was not found in this study group. The leading serotype, Ad3, was identified throughout the duration of the study period. Molecular methods allow for the determination of circulating adenovirus serotypes and be used to document the spread of highly virulent adenoviral serotypes into a region.</p> |
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| format | Article |
| id | doaj.art-6a3a4cad2cdc4433b9ec2f020fde5ac7 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T02:34:00Z |
| publishDate | 2009-01-01 |
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| series | Virology Journal |
| spelling | doaj.art-6a3a4cad2cdc4433b9ec2f020fde5ac72022-12-22T03:06:26ZengBMCVirology Journal1743-422X2009-01-01611110.1186/1743-422X-6-11Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008Mazzulli TonyBlair JoanneLombos ErnestoEshaghi AliRezaYeung RaniBurton LauraDrews Steven J<p>Abstract</p> <p>This study describes the prevalence of culture-positive adenovirus serotypes in culture-positive respiratory specimens sent to the Central Public Health Laboratory, Toronto, Ontario, Canada for the period September 2007–June 2008. Total nucleic acid was extracted from virus cultures using an automated extraction method followed by polymerase chain reaction and Sanger sequencing of the adenovirus hexon gene hypervariable region 7. 73% of specimens (n = 70) were from patients ≤ 4 years of age. Of the 96 adenovirus isolates, the most common identified serotypes were serotype 3 (n = 44, 46%), serotype 2 (n = 25, 26%), serotype 1 (n = 17, 18%), and serotype 21 (n = 5, 5%). Adenovirus serotype 14 was not found in this study group. The leading serotype, Ad3, was identified throughout the duration of the study period. Molecular methods allow for the determination of circulating adenovirus serotypes and be used to document the spread of highly virulent adenoviral serotypes into a region.</p>http://www.virologyj.com/content/6/1/11 |
| spellingShingle | Mazzulli Tony Blair Joanne Lombos Ernesto Eshaghi AliReza Yeung Rani Burton Laura Drews Steven J Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 Virology Journal |
| title | Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 |
| title_full | Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 |
| title_fullStr | Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 |
| title_full_unstemmed | Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 |
| title_short | Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007–June 2008 |
| title_sort | characterization of culture positive adenovirus serotypes from respiratory specimens in toronto ontario canada september 2007 june 2008 |
| url | http://www.virologyj.com/content/6/1/11 |
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