Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis
Transcription of both nuclear and plastid genes encoding components of photosynthetic apparatus is subjected to redox regulation. The origin of redox signals mediating this regulation is chloroplast electron transport chain (ETC) itself. Up to date the effects of redox state of individual ETC compon...
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| Format: | Article |
| Language: | English |
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"Vikol publishing" ST Kolesnichenko V.V.
2016-02-01
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| Series: | Journal of Stress Physiology & Biochemistry |
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| Online Access: | http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_108-118.pdf |
| _version_ | 1823999320369659904 |
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| author | T.V. Yakovleva Y.O. Zubo V.I. Tarasenko E.Yu. Garnik T. Borner Yu.M. Konstantinov |
| author_facet | T.V. Yakovleva Y.O. Zubo V.I. Tarasenko E.Yu. Garnik T. Borner Yu.M. Konstantinov |
| author_sort | T.V. Yakovleva |
| collection | DOAJ |
| description | Transcription of both nuclear and plastid genes encoding components of photosynthetic apparatus is subjected to redox regulation. The origin of redox signals mediating this regulation is chloroplast electron transport chain (ETC) itself. Up to date the effects of redox state of individual ETC components on chloroplast genes transcription rate was demonstrated only for some selected genes and only in isolated chloroplasts. In the present work ETC inhibitors was used for in vivo modulations in plastoquinone pool redox state, and run-on transcription approach was used to evaluate the plastid genes transcription rate. We have demonstrated that plastoquinone redox state has impact on transcription rate of wide range of Arabidopsis chloroplast genes. Treatment by DCMU (plastoquinone pool is oxidized) lead to increase of transcription rate of the majority of plastid genes studied. Treatment by DBMIB (plastoquinone pool is reduced) lead to decrease of plastid genes transcription rate. Simultaneous treatment by one of photosynthesis inhibitors and KCN (mitochondrial respiratory complex IV inhibitor) lead to decrease of transcription rate of plastid genes in most cases. Our results suggest probable participation of mitochondria in the redox regulation of plastid genes expression in Arabidopsis. |
| first_indexed | 2024-12-18T17:42:16Z |
| format | Article |
| id | doaj.art-6a3a80780fd34756aa0536e7f851930e |
| institution | Directory Open Access Journal |
| issn | 1997-0838 1997-0838 |
| language | English |
| last_indexed | 2024-12-18T17:42:16Z |
| publishDate | 2016-02-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-6a3a80780fd34756aa0536e7f851930e2022-12-21T20:59:07Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08381997-08382016-02-01121108118Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in ArabidopsisT.V. Yakovleva0Y.O. Zubo1V.I. Tarasenko2E.Yu. Garnik3T. Borner4Yu.M. Konstantinov5Siberian Institute of Plant Physiology and Biochemistry, SB RAS, Irkutsk, RussiaInstitute of Biology-Genetics, Humboldt University, Berlin, GermanySiberian Institute of Plant Physiology and Biochemistry, SB RAS, Irkutsk, RussiaSiberian Institute of Plant Physiology and Biochemistry, SB RAS, Irkutsk, RussiaInstitute of Biology-Genetics, Humboldt University, Berlin, GermanySiberian Institute of Plant Physiology and Biochemistry, SB RAS, Irkutsk, RussiaTranscription of both nuclear and plastid genes encoding components of photosynthetic apparatus is subjected to redox regulation. The origin of redox signals mediating this regulation is chloroplast electron transport chain (ETC) itself. Up to date the effects of redox state of individual ETC components on chloroplast genes transcription rate was demonstrated only for some selected genes and only in isolated chloroplasts. In the present work ETC inhibitors was used for in vivo modulations in plastoquinone pool redox state, and run-on transcription approach was used to evaluate the plastid genes transcription rate. We have demonstrated that plastoquinone redox state has impact on transcription rate of wide range of Arabidopsis chloroplast genes. Treatment by DCMU (plastoquinone pool is oxidized) lead to increase of transcription rate of the majority of plastid genes studied. Treatment by DBMIB (plastoquinone pool is reduced) lead to decrease of plastid genes transcription rate. Simultaneous treatment by one of photosynthesis inhibitors and KCN (mitochondrial respiratory complex IV inhibitor) lead to decrease of transcription rate of plastid genes in most cases. Our results suggest probable participation of mitochondria in the redox regulation of plastid genes expression in Arabidopsis.http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_108-118.pdfArabidopsis thalianaplastid genes expressionphotosynthetic electron transportDCMUDBMIB |
| spellingShingle | T.V. Yakovleva Y.O. Zubo V.I. Tarasenko E.Yu. Garnik T. Borner Yu.M. Konstantinov Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis Journal of Stress Physiology & Biochemistry Arabidopsis thaliana plastid genes expression photosynthetic electron transport DCMU DBMIB |
| title | Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis |
| title_full | Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis |
| title_fullStr | Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis |
| title_full_unstemmed | Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis |
| title_short | Effect of Photosynthetic Electron Transport Inhibition in vivo on the Chloroplast Genes Transcription in Arabidopsis |
| title_sort | effect of photosynthetic electron transport inhibition in vivo on the chloroplast genes transcription in arabidopsis |
| topic | Arabidopsis thaliana plastid genes expression photosynthetic electron transport DCMU DBMIB |
| url | http://www.jspb.ru/issues/2016/N1/JSPB_2016_1_108-118.pdf |
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