Cross-species functional diversity within the PIN auxin efflux protein family
In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/31804 |
| _version_ | 1811227839008079872 |
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| author | Devin Lee O'Connor Samuel Elton Fabrizio Ticchiarelli Mon Mandy Hsia John P Vogel Ottoline Leyser |
| author_facet | Devin Lee O'Connor Samuel Elton Fabrizio Ticchiarelli Mon Mandy Hsia John P Vogel Ottoline Leyser |
| author_sort | Devin Lee O'Connor |
| collection | DOAJ |
| description | In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the AtPIN1 organ initiation and vein patterning functions are split between the SoPIN1 and PIN1 clades in grasses. Here we show that in the grass Brachypodium sopin1 mutants have organ initiation defects similar to Arabidopsis atpin1, while loss of PIN1 function in Brachypodium has little effect on organ initiation but alters stem growth. Heterologous expression of Brachypodium SoPIN1 and PIN1b in Arabidopsis provides further evidence of functional specificity. SoPIN1 but not PIN1b can mediate flower formation in null atpin1 mutants, although both can complement a missense allele. The behavior of SoPIN1 and PIN1b in Arabidopsis illustrates how membrane and tissue-level accumulation, transport activity, and interaction contribute to PIN functional specificity. |
| first_indexed | 2024-04-12T09:48:46Z |
| format | Article |
| id | doaj.art-6a3ac401ac2f4878800fde8a5f4bb235 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:48:46Z |
| publishDate | 2017-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6a3ac401ac2f4878800fde8a5f4bb2352022-12-22T03:37:53ZengeLife Sciences Publications LtdeLife2050-084X2017-10-01610.7554/eLife.31804Cross-species functional diversity within the PIN auxin efflux protein familyDevin Lee O'Connor0https://orcid.org/0000-0003-4071-8626Samuel Elton1https://orcid.org/0000-0003-4470-4758Fabrizio Ticchiarelli2https://orcid.org/0000-0001-5744-2393Mon Mandy Hsia3John P Vogel4https://orcid.org/0000-0003-1786-2689Ottoline Leyser5https://orcid.org/0000-0003-2161-3829The Sainsbury Laboratory, University of Cambridge, Cambridge, United KingdomThe Sainsbury Laboratory, University of Cambridge, Cambridge, United KingdomThe Sainsbury Laboratory, University of Cambridge, Cambridge, United KingdomWestern Regional Research Center, USDA-ARS, Albany, United StatesUnited States Department of Energy Joint Genome Institute, Walnut Creek, United States; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United StatesThe Sainsbury Laboratory, University of Cambridge, Cambridge, United KingdomIn Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the AtPIN1 organ initiation and vein patterning functions are split between the SoPIN1 and PIN1 clades in grasses. Here we show that in the grass Brachypodium sopin1 mutants have organ initiation defects similar to Arabidopsis atpin1, while loss of PIN1 function in Brachypodium has little effect on organ initiation but alters stem growth. Heterologous expression of Brachypodium SoPIN1 and PIN1b in Arabidopsis provides further evidence of functional specificity. SoPIN1 but not PIN1b can mediate flower formation in null atpin1 mutants, although both can complement a missense allele. The behavior of SoPIN1 and PIN1b in Arabidopsis illustrates how membrane and tissue-level accumulation, transport activity, and interaction contribute to PIN functional specificity.https://elifesciences.org/articles/31804auxinBrachypodiumevo-devoorgan initiationpolaritypatterning |
| spellingShingle | Devin Lee O'Connor Samuel Elton Fabrizio Ticchiarelli Mon Mandy Hsia John P Vogel Ottoline Leyser Cross-species functional diversity within the PIN auxin efflux protein family eLife auxin Brachypodium evo-devo organ initiation polarity patterning |
| title | Cross-species functional diversity within the PIN auxin efflux protein family |
| title_full | Cross-species functional diversity within the PIN auxin efflux protein family |
| title_fullStr | Cross-species functional diversity within the PIN auxin efflux protein family |
| title_full_unstemmed | Cross-species functional diversity within the PIN auxin efflux protein family |
| title_short | Cross-species functional diversity within the PIN auxin efflux protein family |
| title_sort | cross species functional diversity within the pin auxin efflux protein family |
| topic | auxin Brachypodium evo-devo organ initiation polarity patterning |
| url | https://elifesciences.org/articles/31804 |
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