Aberrant DNA Methylation in Keratoacanthoma.
Keratoacanthoma (KA) is a self-limiting epidermal tumor for which histopathological examination sometimes suggests malignancy. Based on inconsistent clinical views, KA can be regarded as both a benign tumor and a variant of squamous cell carcinoma (SCC). Aberrant DNA methylation frequently occurs in...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5082942?pdf=render |
| _version_ | 1819039454557372416 |
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| author | Yoshimasa Nobeyama Hidemi Nakagawa |
| author_facet | Yoshimasa Nobeyama Hidemi Nakagawa |
| author_sort | Yoshimasa Nobeyama |
| collection | DOAJ |
| description | Keratoacanthoma (KA) is a self-limiting epidermal tumor for which histopathological examination sometimes suggests malignancy. Based on inconsistent clinical views, KA can be regarded as both a benign tumor and a variant of squamous cell carcinoma (SCC). Aberrant DNA methylation frequently occurs in malignant tumors but it scarcely occurs in benign tumors. Whether aberrant methylation occurs in KA has not been previously examined.The aim is to elucidate whether aberrant methylation of CpG islands (CGI) containing a high density of cytosine-guanine dinucleotide (CpG) sites occurs in KA.Five SCC cell lines, two cultured samples of normal human epidermal keratinocytes (NHEKs), 18 clinical SCC samples, and 21 clinical KA samples were analyzed with Infinium HumanMethylation450 BeadChips, quantitative real-time methylation-specific PCR (RT-MSP) and/or bisulfite sequencing.Genome-wide analyses of NHEK, KA, and SCC indicated that there was a greater number of aberrantly hypermethylated CGIs in SCC than in KA and there were aberrantly hypermethylated CGIs which are common in both. Among the common hypermethylated CGIs, RT-MSP and bisulfite sequencing targeting CGIs located on CCDC17, PVR, and MAP3K11 gene bodies also showed that methylation levels were significantly higher in KA than in normal epidermis. Statistical analyses suggested that the methylation level of CGI located on PVR in SCC might be correlated to lymph node metastasis (P = 0.013, Mann-Whitney U test) and that the methylation level of CGI in MAP3K11 in KA might be correlated to age (P = 0.031, linear regression analysis).Aberrant DNA methylation occurs in KA. |
| first_indexed | 2024-12-21T08:53:28Z |
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| id | doaj.art-6a3ffcce822c4d5d80a7157f20bde9fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:53:28Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6a3ffcce822c4d5d80a7157f20bde9fc2022-12-21T19:09:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016537010.1371/journal.pone.0165370Aberrant DNA Methylation in Keratoacanthoma.Yoshimasa NobeyamaHidemi NakagawaKeratoacanthoma (KA) is a self-limiting epidermal tumor for which histopathological examination sometimes suggests malignancy. Based on inconsistent clinical views, KA can be regarded as both a benign tumor and a variant of squamous cell carcinoma (SCC). Aberrant DNA methylation frequently occurs in malignant tumors but it scarcely occurs in benign tumors. Whether aberrant methylation occurs in KA has not been previously examined.The aim is to elucidate whether aberrant methylation of CpG islands (CGI) containing a high density of cytosine-guanine dinucleotide (CpG) sites occurs in KA.Five SCC cell lines, two cultured samples of normal human epidermal keratinocytes (NHEKs), 18 clinical SCC samples, and 21 clinical KA samples were analyzed with Infinium HumanMethylation450 BeadChips, quantitative real-time methylation-specific PCR (RT-MSP) and/or bisulfite sequencing.Genome-wide analyses of NHEK, KA, and SCC indicated that there was a greater number of aberrantly hypermethylated CGIs in SCC than in KA and there were aberrantly hypermethylated CGIs which are common in both. Among the common hypermethylated CGIs, RT-MSP and bisulfite sequencing targeting CGIs located on CCDC17, PVR, and MAP3K11 gene bodies also showed that methylation levels were significantly higher in KA than in normal epidermis. Statistical analyses suggested that the methylation level of CGI located on PVR in SCC might be correlated to lymph node metastasis (P = 0.013, Mann-Whitney U test) and that the methylation level of CGI in MAP3K11 in KA might be correlated to age (P = 0.031, linear regression analysis).Aberrant DNA methylation occurs in KA.http://europepmc.org/articles/PMC5082942?pdf=render |
| spellingShingle | Yoshimasa Nobeyama Hidemi Nakagawa Aberrant DNA Methylation in Keratoacanthoma. PLoS ONE |
| title | Aberrant DNA Methylation in Keratoacanthoma. |
| title_full | Aberrant DNA Methylation in Keratoacanthoma. |
| title_fullStr | Aberrant DNA Methylation in Keratoacanthoma. |
| title_full_unstemmed | Aberrant DNA Methylation in Keratoacanthoma. |
| title_short | Aberrant DNA Methylation in Keratoacanthoma. |
| title_sort | aberrant dna methylation in keratoacanthoma |
| url | http://europepmc.org/articles/PMC5082942?pdf=render |
| work_keys_str_mv | AT yoshimasanobeyama aberrantdnamethylationinkeratoacanthoma AT hideminakagawa aberrantdnamethylationinkeratoacanthoma |