The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions
Harmful cyanobacterial blooms pose a major threat. Among them, <i>Microcystis aeruginosa</i> has raised serious concerns for human health due to its frequent occurrence. In this study, an ecological floating-bed system consisting of activated carbon fibers, aquatic plants (Ipomoea aquati...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/15/20/3607 |
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| author | Guoming Zeng Dong Liang Cheng Tang Yuanyuan Huang Da Sun |
| author_facet | Guoming Zeng Dong Liang Cheng Tang Yuanyuan Huang Da Sun |
| author_sort | Guoming Zeng |
| collection | DOAJ |
| description | Harmful cyanobacterial blooms pose a major threat. Among them, <i>Microcystis aeruginosa</i> has raised serious concerns for human health due to its frequent occurrence. In this study, an ecological floating-bed system consisting of activated carbon fibers, aquatic plants (Ipomoea aquatica Forsskal), animals (Daphnia), and a solar-powered ultrasonic device was designed. The algae-killing efficiency, removal mechanism, and toxicological effects of the floating-bed system on <i>Microcystis aeruginosa</i> were determined under different conditions. The results showed that the average activity of algal cell dehydrogenase (DHA) was reduced by 64.09%, the average malondialdehyde (MDA) content was reduced to 0.125 μmol/L, and the average removal rate of soluble protein was 57.22% under optimal conditions (pH = 7, temperature = 25 °C, dissolved oxygen concentration = 5 mg/L, and hydraulic retention time = 36 h). Scanning electron microscopy (SEM) analysis showed that the structure within the cells of <i>Microcystis aeruginosa</i> was severely damaged after treatment with the solar-powered ultrasonic carbon fiber eco-floating-bed system. Fourier transform infrared (FTIR) spectroscopy analysis showed that the pyrrole ring of chlorophyll-a was degraded. In addition, a tadpole-based micronucleus test and a comet assay were conducted to assess cell viability and DNA damage in water samples treated with the floating-bed system, and the results confirmed that chromosome damage and genotoxicity were significantly reduced. These findings suggest that the floating-bed system is effective in destroying algal cells, leading to massive algal cell death and reducing the risk of secondary contamination. This study provides a new perspective for further research on ecological floating-bed technology, demonstrating its potential practical application in the prevention and control of cyanobacterial blooms. |
| first_indexed | 2024-03-10T20:48:51Z |
| format | Article |
| id | doaj.art-6a40568e0b254a728f2572f319781bc8 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T20:48:51Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-6a40568e0b254a728f2572f319781bc82023-11-19T18:30:11ZengMDPI AGWater2073-44412023-10-011520360710.3390/w15203607The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory ConditionsGuoming Zeng0Dong Liang1Cheng Tang2Yuanyuan Huang3Da Sun4Intelligent Construction Technology Application Service Center, School of Architecture and Engineering, Chongqing City Vocational College, Chongqing 402160, ChinaSchool of Architecture and Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Architecture and Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaCollege of River and Ocean Engineering, Chongqing Jiaotong University, Chongqing 400074, ChinaNational & Local Joint Engineering Research Center for Ecological Treatment Technology of Urban Water, Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources, Wenzhou University, Wenzhou 325035, ChinaHarmful cyanobacterial blooms pose a major threat. Among them, <i>Microcystis aeruginosa</i> has raised serious concerns for human health due to its frequent occurrence. In this study, an ecological floating-bed system consisting of activated carbon fibers, aquatic plants (Ipomoea aquatica Forsskal), animals (Daphnia), and a solar-powered ultrasonic device was designed. The algae-killing efficiency, removal mechanism, and toxicological effects of the floating-bed system on <i>Microcystis aeruginosa</i> were determined under different conditions. The results showed that the average activity of algal cell dehydrogenase (DHA) was reduced by 64.09%, the average malondialdehyde (MDA) content was reduced to 0.125 μmol/L, and the average removal rate of soluble protein was 57.22% under optimal conditions (pH = 7, temperature = 25 °C, dissolved oxygen concentration = 5 mg/L, and hydraulic retention time = 36 h). Scanning electron microscopy (SEM) analysis showed that the structure within the cells of <i>Microcystis aeruginosa</i> was severely damaged after treatment with the solar-powered ultrasonic carbon fiber eco-floating-bed system. Fourier transform infrared (FTIR) spectroscopy analysis showed that the pyrrole ring of chlorophyll-a was degraded. In addition, a tadpole-based micronucleus test and a comet assay were conducted to assess cell viability and DNA damage in water samples treated with the floating-bed system, and the results confirmed that chromosome damage and genotoxicity were significantly reduced. These findings suggest that the floating-bed system is effective in destroying algal cells, leading to massive algal cell death and reducing the risk of secondary contamination. This study provides a new perspective for further research on ecological floating-bed technology, demonstrating its potential practical application in the prevention and control of cyanobacterial blooms.https://www.mdpi.com/2073-4441/15/20/3607ecological floating bed<i>Microcystis aeruginosa</i>aquatic animals and plantspollutant removalmicronucleus testcomet assay |
| spellingShingle | Guoming Zeng Dong Liang Cheng Tang Yuanyuan Huang Da Sun The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions Water ecological floating bed <i>Microcystis aeruginosa</i> aquatic animals and plants pollutant removal micronucleus test comet assay |
| title | The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions |
| title_full | The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions |
| title_fullStr | The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions |
| title_full_unstemmed | The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions |
| title_short | The Algicidal Potential of a Floating-Bed System against <i>Microcystis aeruginosa</i> in Laboratory Conditions |
| title_sort | algicidal potential of a floating bed system against i microcystis aeruginosa i in laboratory conditions |
| topic | ecological floating bed <i>Microcystis aeruginosa</i> aquatic animals and plants pollutant removal micronucleus test comet assay |
| url | https://www.mdpi.com/2073-4441/15/20/3607 |
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