Evolutionary and Structural Analysis of PP16 in Viridiplantae
Members of the phloem protein 16 (PP16) gene family are induced by elicitors in rice and the corresponding proteins from cucurbits, which display RNA binding and intercellular transport activities, are accumulated in phloem sap. These proteins facilitate the movement of protein complexes through the...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/2839 |
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| author | Domingo Jiménez-López Beatriz Xoconostle-Cázares Berenice Calderón-Pérez Brenda Yazmín Vargas-Hernández Leandro Alberto Núñez-Muñoz José Abrahán Ramírez-Pool Roberto Ruiz-Medrano |
| author_facet | Domingo Jiménez-López Beatriz Xoconostle-Cázares Berenice Calderón-Pérez Brenda Yazmín Vargas-Hernández Leandro Alberto Núñez-Muñoz José Abrahán Ramírez-Pool Roberto Ruiz-Medrano |
| author_sort | Domingo Jiménez-López |
| collection | DOAJ |
| description | Members of the phloem protein 16 (PP16) gene family are induced by elicitors in rice and the corresponding proteins from cucurbits, which display RNA binding and intercellular transport activities, are accumulated in phloem sap. These proteins facilitate the movement of protein complexes through the phloem translocation flow and may be involved in the response to water deficit, among other functions. However, there is scant information regarding their function in other plants, including the identification of paralog genes in non-vascular plants and chlorophytes. In the present work, an evolutionary and structural analysis of the PP16 family in green plants (Viridiplantae) was carried out. Data mining in different databases indicated that <i>PP16</i> likely originated from a larger gene present in an ancestral lineage that gave rise to chlorophytes and multicellular plants. This gene encodes a protein related to synaptotagmin, which is involved in vesicular transport in animal systems, although other members of this family play a role in lipid turnover in endomembranes and organelles. These proteins contain a membrane-binding C2 domain shared with PP16 proteins in vascular plants. In silico analysis of the predicted structure of the PP16 protein family identified several β-sheets, one α-helix, and intrinsically disordered regions. PP16 may have been originally involved in vesicular trafficking and/or membrane maintenance but specialized in long-distance signaling during the emergence of the plant vascular system. |
| first_indexed | 2024-04-25T00:27:43Z |
| format | Article |
| id | doaj.art-6a42a971e4fb4eca801857cfea6350c5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:27:43Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6a42a971e4fb4eca801857cfea6350c52024-03-12T16:46:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255283910.3390/ijms25052839Evolutionary and Structural Analysis of PP16 in ViridiplantaeDomingo Jiménez-López0Beatriz Xoconostle-Cázares1Berenice Calderón-Pérez2Brenda Yazmín Vargas-Hernández3Leandro Alberto Núñez-Muñoz4José Abrahán Ramírez-Pool5Roberto Ruiz-Medrano6Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoMembers of the phloem protein 16 (PP16) gene family are induced by elicitors in rice and the corresponding proteins from cucurbits, which display RNA binding and intercellular transport activities, are accumulated in phloem sap. These proteins facilitate the movement of protein complexes through the phloem translocation flow and may be involved in the response to water deficit, among other functions. However, there is scant information regarding their function in other plants, including the identification of paralog genes in non-vascular plants and chlorophytes. In the present work, an evolutionary and structural analysis of the PP16 family in green plants (Viridiplantae) was carried out. Data mining in different databases indicated that <i>PP16</i> likely originated from a larger gene present in an ancestral lineage that gave rise to chlorophytes and multicellular plants. This gene encodes a protein related to synaptotagmin, which is involved in vesicular transport in animal systems, although other members of this family play a role in lipid turnover in endomembranes and organelles. These proteins contain a membrane-binding C2 domain shared with PP16 proteins in vascular plants. In silico analysis of the predicted structure of the PP16 protein family identified several β-sheets, one α-helix, and intrinsically disordered regions. PP16 may have been originally involved in vesicular trafficking and/or membrane maintenance but specialized in long-distance signaling during the emergence of the plant vascular system.https://www.mdpi.com/1422-0067/25/5/2839phloem protein 16phylogenyphloem transportnon-cell autonomous protein |
| spellingShingle | Domingo Jiménez-López Beatriz Xoconostle-Cázares Berenice Calderón-Pérez Brenda Yazmín Vargas-Hernández Leandro Alberto Núñez-Muñoz José Abrahán Ramírez-Pool Roberto Ruiz-Medrano Evolutionary and Structural Analysis of PP16 in Viridiplantae International Journal of Molecular Sciences phloem protein 16 phylogeny phloem transport non-cell autonomous protein |
| title | Evolutionary and Structural Analysis of PP16 in Viridiplantae |
| title_full | Evolutionary and Structural Analysis of PP16 in Viridiplantae |
| title_fullStr | Evolutionary and Structural Analysis of PP16 in Viridiplantae |
| title_full_unstemmed | Evolutionary and Structural Analysis of PP16 in Viridiplantae |
| title_short | Evolutionary and Structural Analysis of PP16 in Viridiplantae |
| title_sort | evolutionary and structural analysis of pp16 in viridiplantae |
| topic | phloem protein 16 phylogeny phloem transport non-cell autonomous protein |
| url | https://www.mdpi.com/1422-0067/25/5/2839 |
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