Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation
Mesenchymal stem cell (MSC)-derived exosomes have diverse functions in regulating wound healing and inflammation; however, the molecular mechanism of human umbilical cord MSC (hUCMSC)-derived exosomes in regulating burn-induced inflammation is not well understood. We found that burn injury significa...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | EBioMedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396416301736 |
| _version_ | 1819027018323329024 |
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| author | Xiao Li Lingying Liu Jing Yang Yonghui Yu Jiake Chai Lingyan Wang Li Ma Huinan Yin |
| author_facet | Xiao Li Lingying Liu Jing Yang Yonghui Yu Jiake Chai Lingyan Wang Li Ma Huinan Yin |
| author_sort | Xiao Li |
| collection | DOAJ |
| description | Mesenchymal stem cell (MSC)-derived exosomes have diverse functions in regulating wound healing and inflammation; however, the molecular mechanism of human umbilical cord MSC (hUCMSC)-derived exosomes in regulating burn-induced inflammation is not well understood. We found that burn injury significantly increased the inflammatory reaction of rats or macrophages exposed to lipopolysaccharide (LPS), increased tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) levels and decreased IL-10 levels. hUCMSC-exosome administration successfully reversed this reaction. Further studies showed that miR-181c in the exosomes played a pivotal role in regulating inflammation. Compared to control hUCMSC-exosomes, hUCMSC-exosomes overexpressing miR-181c more effectively suppressed the TLR4 signaling pathway and alleviated inflammation in burned rats. Administration of miR-181c-expressing hUCMSC-exosomes or TLR4 knockdown significantly reduced LPS-induced TLR4 expression by macrophages and the inflammatory reaction. In summary, miR-181c expression in hUCMSC-exosomes reduces burn-induced inflammation by downregulating the TLR4 signaling pathway. |
| first_indexed | 2024-12-21T05:35:48Z |
| format | Article |
| id | doaj.art-6a441532467146edbf6f803a7cef8291 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-21T05:35:48Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-6a441532467146edbf6f803a7cef82912022-12-21T19:14:24ZengElsevierEBioMedicine2352-39642016-06-018C728210.1016/j.ebiom.2016.04.030Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive InflammationXiao Li0Lingying Liu1Jing Yang2Yonghui Yu3Jiake Chai4Lingyan Wang5Li Ma6Huinan Yin7Department of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Medical Administration, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaDepartment of Burn & Plastic Surgery, The First Affiliated Hospital to PLA General Hospital, Beijing 100048, ChinaMesenchymal stem cell (MSC)-derived exosomes have diverse functions in regulating wound healing and inflammation; however, the molecular mechanism of human umbilical cord MSC (hUCMSC)-derived exosomes in regulating burn-induced inflammation is not well understood. We found that burn injury significantly increased the inflammatory reaction of rats or macrophages exposed to lipopolysaccharide (LPS), increased tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) levels and decreased IL-10 levels. hUCMSC-exosome administration successfully reversed this reaction. Further studies showed that miR-181c in the exosomes played a pivotal role in regulating inflammation. Compared to control hUCMSC-exosomes, hUCMSC-exosomes overexpressing miR-181c more effectively suppressed the TLR4 signaling pathway and alleviated inflammation in burned rats. Administration of miR-181c-expressing hUCMSC-exosomes or TLR4 knockdown significantly reduced LPS-induced TLR4 expression by macrophages and the inflammatory reaction. In summary, miR-181c expression in hUCMSC-exosomes reduces burn-induced inflammation by downregulating the TLR4 signaling pathway.http://www.sciencedirect.com/science/article/pii/S2352396416301736ExosomemiR-181cBurnInflammation |
| spellingShingle | Xiao Li Lingying Liu Jing Yang Yonghui Yu Jiake Chai Lingyan Wang Li Ma Huinan Yin Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation EBioMedicine Exosome miR-181c Burn Inflammation |
| title | Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation |
| title_full | Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation |
| title_fullStr | Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation |
| title_full_unstemmed | Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation |
| title_short | Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation |
| title_sort | exosome derived from human umbilical cord mesenchymal stem cell mediates mir 181c attenuating burn induced excessive inflammation |
| topic | Exosome miR-181c Burn Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2352396416301736 |
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